{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:25:05Z","timestamp":1790652305138,"version":"4.1.0"},"reference-count":86,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T00:00:00Z","timestamp":1645142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007487","name":"BASF (United States)","doi-asserted-by":"publisher","award":["AT-56258"],"award-info":[{"award-number":["AT-56258"]}],"id":[{"id":"10.13039\/100007487","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Prader\u2013Willi Syndrome (PWS) is a human genetic condition that affects up to 1 in 10,000 live births. Affected infants present with hypotonia and developmental delay. Hyperphagia and increasing body weight follow unless drastic calorie restriction is initiated. Recently, our laboratory showed that one of the genes in the deleted locus causative for PWS, Snord116, maintains increased expression of hypothalamic Nhlh2, a basic helix\u2013loop\u2013helix transcription factor. We have previously also shown that obese mice with a deletion of Nhlh2 respond to a conjugated linoleic acid (CLA) diet with weight and fat loss. In this study, we investigated whether mice with a paternal deletion of Snord116 (Snord116m+\/p\u2212) would respond similarly. We found that while Snord116m+\/p\u2212 mice and mice with a deletion of both Snord116 alleles were not significantly obese on a high-fat diet, they did lose body weight and fat on a high-fat\/CLA diet, suggesting that the genotype did not interfere with CLA actions. There were no changes in food intake or metabolic rate, and only moderate differences in exercise performance. RNA-seq and microbiome analyses identified hypothalamic mRNAs, and differentially populated gut bacteria, that support future mechanistic analyses. CLA may be useful as a food additive to reduce obesity in humans with PWS.<\/jats:p>","DOI":"10.3390\/nu14040860","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T08:22:19Z","timestamp":1645431739000},"page":"860","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Dietary Conjugated Linoleic Acid Reduces Body Weight and Fat in Snord116m+\/p\u2212 and Snord116m\u2212\/p\u2212 Mouse Models of Prader\u2013Willi Syndrome"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9111-7383","authenticated-orcid":false,"given":"Brittney","family":"Knott","sequence":"first","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA 24060, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Kocher","sequence":"additional","affiliation":[{"name":"Translational Biology, Medicine and Health Graduate Program, Virginia Tech, Roanoke, VA 24016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry A.","family":"Paz","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, College of Medicine, University of Arkansas Medical Center, Little Rock, AR 72202, USA"},{"name":"Arkansas Children Nutrition Center, Arkansas Children\u2019s Research Institute, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8676-3639","authenticated-orcid":false,"given":"Shelby E.","family":"Hamm","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA 24060, USA"},{"name":"Metabolism Core Facility, Virginia Tech, Blacksburg, VA 24060, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Fink","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA 24060, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA 24060, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Grange","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA 24060, USA"},{"name":"Metabolism Core Facility, Virginia Tech, Blacksburg, VA 24060, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umesh D.","family":"Wankhade","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, College of Medicine, University of Arkansas Medical Center, Little Rock, AR 72202, USA"},{"name":"Arkansas Children Nutrition Center, Arkansas Children\u2019s Research Institute, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0136-0975","authenticated-orcid":false,"given":"Deborah J.","family":"Good","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA 24060, USA"},{"name":"Translational Biology, Medicine and Health Graduate Program, Virginia Tech, Roanoke, VA 24016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e138576","DOI":"10.1172\/jci.insight.138576","article-title":"Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production","volume":"5","author":"Chen","year":"2020","journal-title":"JCI Insight"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Tan, Q., Potter, K.J., Burnett, L.C., Orsso, C.E., Inman, M., Ryman, D.C., and Haqq, A.M. 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