{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T16:17:54Z","timestamp":1769271474887,"version":"3.49.0"},"reference-count":48,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Non-alcoholic fatty liver disease (NAFLD) is a multifactorial condition characterized from hypertriglyceridemia and hepatic fat accumulation, in the absence of alcohol intake. NAFLD starts as steatosis (NAFL), and the continued injury relative to the toxic fat induces inflammation, steatohepatitis (NASH), and HCC. One of the factors determining liver degeneration during the evolution of NAFLD is a modification of Wnt\/Frizzled (FZD) signaling. In particular, an inhibition of Wnt signaling and an overexpression of a specific FZD receptor protein, namely, the FZD7, have been observed in NAFLD. Actually, the prognosis and the follow-up of NAFLD is not easy, and the liver biopsy is the gold standard for an accurate detection of liver fibrosis. In this study, the modulation of the FZD7 expression levels in plasma-derived exosomes of NAFLD-affected patients, before and after specific lifestyle interventions, were experimentally evaluated by Western blotting analysis. The experimental data were analyzed by an accurate statistical study that indicated, in the exosomes derived from plasma of NAFLD patients with moderate or severe steatosis, an average expression level of FZD7 that was significantly higher than healthy subjects at baseline; conversely, the values were normalized after 90 days of specific lifestyle interventions. The overall results suggested that the FZD7 delivered by exosomes represents a good candidate as a new and effective biomarker for diagnosis and prognosis of NAFLD.<\/jats:p>","DOI":"10.3390\/nu14061133","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T01:53:54Z","timestamp":1646790834000},"page":"1133","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Exosomal FZD-7 Expression Is Modulated by Different Lifestyle Interventions in Patients with NAFLD"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0812-4441","authenticated-orcid":false,"given":"Maria","family":"Scavo","sequence":"first","affiliation":[{"name":"Laboratory of Personalized Medicine, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2107-2762","authenticated-orcid":false,"given":"Nicoletta","family":"Depalo","sequence":"additional","affiliation":[{"name":"Institute for Chemical-Physical Processes, Italian National Research Council (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8891-4218","authenticated-orcid":false,"given":"Federica","family":"Rizzi","sequence":"additional","affiliation":[{"name":"Institute for Chemical-Physical Processes, Italian National Research Council (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, Italy"},{"name":"Dipartimento di Chimica, Universit\u00e0 degli Studi di Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6769-9520","authenticated-orcid":false,"given":"Livianna","family":"Carrieri","sequence":"additional","affiliation":[{"name":"Laboratory of Personalized Medicine, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2971-0802","authenticated-orcid":false,"given":"Grazia","family":"Serino","sequence":"additional","affiliation":[{"name":"Molecular Medicine, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3697-0127","authenticated-orcid":false,"given":"Isabella","family":"Franco","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Biostatistics, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8650-348X","authenticated-orcid":false,"given":"Caterina","family":"Bonfiglio","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Biostatistics, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4675-2075","authenticated-orcid":false,"given":"Pasqua","family":"Pesole","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaele","family":"Cozzolongo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Castellana Grotte, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vito","family":"Gianuzzi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Castellana Grotte, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Curri","sequence":"additional","affiliation":[{"name":"Institute for Chemical-Physical Processes, Italian National Research Council (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, Italy"},{"name":"Dipartimento di Chimica, Universit\u00e0 degli Studi di Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4232-8760","authenticated-orcid":false,"given":"Alberto","family":"Osella","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Biostatistics, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluigi","family":"Giannelli","sequence":"additional","affiliation":[{"name":"Scientific Direction, National Institute of Gastroenterology IRCCS \u201cS. de Bellis\u201d, Research Hospital, Castellana Grotte, 70013 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1136\/gutjnl-2017-315691","article-title":"Non-alcoholic steatohepatitis pathogenesis: Sublethal hepatocyte injury as a driver of liver inflammation","volume":"67","author":"Ibrahim","year":"2018","journal-title":"Gut"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1136\/gutjnl-2017-313884","article-title":"Non-alcoholic fatty liver disease and its relationship with cardiovascular disease and other extrahepatic 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