{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T08:30:17Z","timestamp":1778574617109,"version":"3.51.4"},"reference-count":31,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Institute of Oriental Medicine","award":["KSN2022240"],"award-info":[{"award-number":["KSN2022240"]}]},{"name":"Korea Institute of Oriental Medicine","award":["C18010"],"award-info":[{"award-number":["C18010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Dried ginger (Zingiberis Processum Rhizoma (ZR)) is frequently used to prevent or treat common cold and flu. This study aimed to investigate the influence of ZR extracts on influenza-specific antibody production in cyclophosphamide (Cy)-induced immunocompromised mice. Female BALB\/c mice were injected three times with saline or Cy. To investigate the effect of ZR, either distilled water or ZR was administered orally to mice daily for 10 days after Cy injection. After ZR administration, the mice were immunized with the 2017\/2018 influenza vaccine. Pretreatment with ZR extracts enhanced influenza-specific antibody production in Cy-induced immunocompromised mice after flu vaccination and restored the influenza antigen-specific T helper (Th) type 1\/Th2 balance to the normal state. Further, ZR suppressed the eosinophil enrichment caused by Cy injection in the spleen. We demonstrated that ZR can be used to increase antibody production in immunocompromised individuals before vaccination.<\/jats:p>","DOI":"10.3390\/nu14091984","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T00:30:28Z","timestamp":1652142628000},"page":"1984","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Dried Ginger Extract Restores the T Helper Type 1\/T Helper Type 2 Balance and Antibody Production in Cyclophosphamide-Induced Immunocompromised Mice after Flu Vaccination"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2466-1925","authenticated-orcid":false,"given":"Jihyun","family":"Kim","sequence":"first","affiliation":[{"name":"KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6948-892X","authenticated-orcid":false,"given":"Sooseong","family":"You","sequence":"additional","affiliation":[{"name":"KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2019). Global Influenza Strategy 2019\u20132030, World Health Organization (WHO)."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1080\/21645515.2018.1445446","article-title":"Seasonal influenza vaccine in immunocompromised persons","volume":"14","author":"Bosaeed","year":"2018","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S1473-3099(09)70175-6","article-title":"Influenza in immunosuppressed populations: A review of infection frequency, morbidity, mortality, and vaccine responses","volume":"9","author":"Kunisaki","year":"2009","journal-title":"Lancet Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.bbmt.2015.12.003","article-title":"Randomized double-blind study of the safety and immunogenicity of standard-dose trivalent inactivated influenza vaccine versus high-dose trivalent inactivated influenza vaccine in adult hematopoietic stem cell transplantation patients","volume":"22","author":"Halasa","year":"2016","journal-title":"Biol. Blood Marrow Transplant."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1097\/TP.0000000000000861","article-title":"Randomized controlled trial of adjuvanted versus nonadjuvanted influenza vaccine in kidney transplant recipients","volume":"100","author":"Kumar","year":"2016","journal-title":"Transplantation"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1080\/21645515.2016.1191717","article-title":"Immunogenicity of trivalent influenza vaccines in patients with chronic kidney disease undergoing hemodialysis: MF59-adjuvanted versus non-adjuvanted vaccines","volume":"12","author":"Noh","year":"2016","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1093\/cid\/cix1082","article-title":"A double-blind, randomized trial of high-dose vs standard-dose influenza vaccine in adult solid-organ transplant recipients","volume":"66","author":"Natori","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"96","DOI":"10.5808\/GI.2016.14.3.96","article-title":"Identification of suitable natural inhibitor against influenza A (H1N1) neuraminidase protein by molecular docking","volume":"14","author":"Sahoo","year":"2016","journal-title":"Genom. Inform."},{"key":"ref_9","first-page":"267","article-title":"Therapeutic potential of ginger against COVID-19: Is there enough evidence?","volume":"8","author":"Jafarzadeh","year":"2021","journal-title":"J. Tradit. Chin. Med. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e13612","DOI":"10.1111\/jfbc.13612","article-title":"Effect of ginger (Zingiber officinale) supplementation on oxidative stress parameters: A systematic review and meta-analysis","volume":"45","author":"Morvaridzadeh","year":"2021","journal-title":"J. Food Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"155224","DOI":"10.1016\/j.cyto.2020.155224","article-title":"Effect of ginger (Zingiber officinale) on inflammatory markers: A systematic review and meta-analysis of randomized controlled trials","volume":"135","author":"Morvaridzadeh","year":"2020","journal-title":"Cytokine"},{"key":"ref_12","first-page":"1341","article-title":"Anti-avian influenza virus H9N2 activity of aqueous extracts of Zingiber officinalis (Ginger) and Allium sativum (Garlic) in chick embryos","volume":"30","author":"Rasool","year":"2017","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.jep.2012.10.043","article-title":"Fresh ginger (Zingiber officinale) has anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines","volume":"145","author":"Chang","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1002\/ptr.6134","article-title":"Protective and therapeutic potential of ginger (Zingiber officinale) extract and [6]-gingerol in cancer: A comprehensive review","volume":"32","author":"Santos","year":"2018","journal-title":"Phytother. Res."},{"key":"ref_15","first-page":"S36","article-title":"Anti-Oxidative and Anti-Inflammatory Effects of Ginger in Health and Physical Activity: Review of Current Evidence","volume":"4","author":"Mashhadi","year":"2013","journal-title":"Int. J. Prev. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1016\/j.intimp.2008.07.009","article-title":"Ginger prevents Th2-mediated immune responses in a mouse model of airway inflammation","volume":"8","author":"Ahui","year":"2008","journal-title":"Int. Immunopharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"18007","DOI":"10.1038\/s41598-018-36703-7","article-title":"Induction of human t-cell and cytokine responses following vaccination with a novel influenza vaccine","volume":"8","author":"Skibinski","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1038\/ni.3563","article-title":"Protective neutralizing influenza antibody response in the absence of T follicular helper cells","volume":"17","author":"Miyauchi","year":"2016","journal-title":"Nat. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1080\/10408391003624848","article-title":"Ginger and its health claims: Molecular aspects","volume":"51","author":"Butt","year":"2011","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1290\/1543-706X(2004)040<0095:AIVSAF>2.0.CO;2","article-title":"An in vitro screening assay for inhibitors of proinflammatory mediators in herbal extracts using human synoviocyte cultures","volume":"40","author":"Frondoza","year":"2004","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jchromb.2016.01.009","article-title":"Chemical characterization and antioxidant activities comparison in fresh, dried, stir-frying and carbonized ginger","volume":"1011","author":"Li","year":"2016","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1002\/cyto.a.22012","article-title":"A novel Ly6C\/Ly6G-based strategy to analyze the mouse splenic myeloid compartment","volume":"81","author":"Rose","year":"2012","journal-title":"Cytom. A"},{"key":"ref_23","first-page":"261","article-title":"Eosinophilia after allogeneic bone marrow transplantation using busulfan and cyclophosphamide conditioning regimen","volume":"18","author":"Arslan","year":"1996","journal-title":"Bone Marrow Transpl."},{"key":"ref_24","first-page":"383","article-title":"Cyclophosphamide-induced eosinophilia in the rat: Concomitant changes in T-cell subsets, B cells and large granular lymphocytes within lymphoid tissues","volume":"60","author":"Thomson","year":"1987","journal-title":"Immunology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/j.1365-3083.1987.tb02274.x","article-title":"Generation of large granular lymphocytes and lymphocyte subset changes linked with cyclophosphamide-induced eosinophilia in rats--and the effects of ciclosporin","volume":"26","author":"Mathie","year":"1987","journal-title":"Scand. J. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/icb.2009.115","article-title":"Eosinophils and Th2 immunity: Contemporary insights","volume":"88","author":"Spencer","year":"2010","journal-title":"Immunol. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1189\/jlb.0108058","article-title":"Human eosinophils constitutively express multiple Th1, Th2, and immunoregulatory cytokines that are secreted rapidly and differentially","volume":"85","author":"Spencer","year":"2009","journal-title":"J. Leukoc. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Mao, Q.Q., Xu, X.Y., Cao, S.Y., Gan, R.Y., Corke, H., Beta, T., and Li, H.B. (2019). Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods, 8.","DOI":"10.3390\/foods8060185"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Bischoff-Kont, I., and F\u00fcrst, R. (2021). Benefits of ginger and its constituent 6-Shogaol in inhibiting inflammatory processes. Pharmaceuticals, 14.","DOI":"10.3390\/ph14060571"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"12869","DOI":"10.33263\/BRIAC115.1286912876","article-title":"Shogaol, Bisdemethoxycurcumin, and Curcuminoid: Potential zingiber compounds against COVID-19","volume":"11","author":"Afrida","year":"2021","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s43094-020-00171-6","article-title":"Compounds of citrus medica and zingiber officinale for COVID-19 inhibition: In silico evidence for cues from ayurveda","volume":"7","author":"Haridas","year":"2021","journal-title":"Future J. Pharm. Sci."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/9\/1984\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:08:20Z","timestamp":1760137700000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/9\/1984"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,9]]},"references-count":31,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["nu14091984"],"URL":"https:\/\/doi.org\/10.3390\/nu14091984","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,9]]}}}