{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:43:38Z","timestamp":1793321018823,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Postnatally, short-chain fatty acids (SCFA) are important energetic and signaling agents, being involved in host nutrition, gut imprinting and immune and barrier function. Whether SCFA exert similar effects during the late fetal phase has been insufficiently elucidated. This study aimed to evaluate whether the fetal jejunum senses SCFA and whether SCFA modify the muscle tension and epithelial permeability and related signaling in jejunal tissue from the porcine fetus in late gestation. Exposure of fetal jejunal tissue to a mix of SCFA (70 \u00b5mol\/mL) in an organ bath for 20 min lowered the muscle tension. Moreover, SCFA decreased the transepithelial conductance while increasing the short-circuit current in the Ussing chamber, indicating reduced permeability and increased SCFA absorption. Gene expression in the tissues harvested from the Ussing chamber after 30 min indicated downregulation of the expression of receptors (i.e., FFAR2 and TLR2), MCT1 and tight-junction and adherens proteins, which may be a negative feedback response to the applied high SCFA concentration compared with the micromolar concentration detected in fetal gastric fluid. Taken together, our data demonstrate that the fetal jejunum senses SCFA, which trigger electrophysiological, muscle contraction and related gene transcription responses. Hence, SCFA may play a role in prenatal gut nutrition and imprinting.<\/jats:p>","DOI":"10.3390\/nu14122524","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T11:45:44Z","timestamp":1655466344000},"page":"2524","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Short-Chain Fatty Acids Modulate Permeability, Motility and Gene Expression in the Porcine Fetal Jejunum Ex Vivo"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2413-4957","authenticated-orcid":false,"given":"Barbara U.","family":"Metzler-Zebeli","sequence":"first","affiliation":[{"name":"Unit of Nutritional Physiology, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"},{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Koger","sequence":"additional","affiliation":[{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"},{"name":"Institute of Animal Nutrition and Functional Plant Compounds, Department of Farm Animal and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suchitra","family":"Sharma","sequence":"additional","affiliation":[{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"},{"name":"Institute of Animal Nutrition and Functional Plant Compounds, Department of Farm Animal and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arife","family":"Sener-Aydemir","sequence":"additional","affiliation":[{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"},{"name":"Institute of Animal Nutrition and Functional Plant Compounds, Department of Farm Animal and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Ruczizka","sequence":"additional","affiliation":[{"name":"University Clinic for Swine, Department of Farm Animal and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"},{"name":"Association for Sustainable Animal Husbandry in Austria (NT\u00d6)\u2013Project Animal Health Austria, 1200 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinrich","family":"Kreutzmann","sequence":"additional","affiliation":[{"name":"University Clinic for Swine, Department of Farm Animal and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ladinig","sequence":"additional","affiliation":[{"name":"University Clinic for Swine, Department of Farm Animal and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1017\/S0007114512000414","article-title":"High-protein-low-carbohydrate diet during pregnancy alters maternal plasma amino acid concentration and placental amino acid extraction but not fetal plasma amino acids in pigs","volume":"108","author":"Lang","year":"2012","journal-title":"Br. 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