{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:12:13Z","timestamp":1791616333219,"version":"4.3.4"},"reference-count":49,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004391","name":"CMU Hospital","doi-asserted-by":"publisher","award":["DMR-111-108"],"award-info":[{"award-number":["DMR-111-108"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004391","name":"CMU Hospital","doi-asserted-by":"publisher","award":["DMR-111-229"],"award-info":[{"award-number":["DMR-111-229"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004391","name":"CMU Hospital","doi-asserted-by":"publisher","award":["CMUHCH-DMR-111-010"],"award-info":[{"award-number":["CMUHCH-DMR-111-010"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CMU Hsinchu Hospital","award":["DMR-111-108"],"award-info":[{"award-number":["DMR-111-108"]}]},{"name":"CMU Hsinchu Hospital","award":["DMR-111-229"],"award-info":[{"award-number":["DMR-111-229"]}]},{"name":"CMU Hsinchu Hospital","award":["CMUHCH-DMR-111-010"],"award-info":[{"award-number":["CMUHCH-DMR-111-010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Osteoarthritis (OA) is a painful, progressive chronic inflammatory disease marked by cartilage destruction. Certain synovial inflammatory cytokines, such as IL-1\u03b2 and TNF-\u03b1, promote OA inflammation and pain. Lactobacillus spp. is a well-known probiotic with anti-inflammatory, analgesic, antioxidant, and antiosteoporotic properties. This study evaluated the therapeutic effects of a live L. plantarum strain (GKD7) in the anterior cruciate ligament transection (ACLT)-induced OA rat model. The results show that oral administration of live L. plantarum GKD7 improved weight-bearing asymmetry after ACLT surgery. Moreover, micro-computed tomography images and histopathological analysis show that oral live L. plantarum GKD7 improved subchondral bone architecture, protected articular cartilage against ACLT-induced damage, and reduced synovial inflammation. L. plantarum GKD7 also reduced IL-1\u03b2 and TNF-\u03b1 production in OA cartilage and synovium. Thus, orally administered live L. plantarum GKD7 appears to effectively slow the progression of OA.<\/jats:p>","DOI":"10.3390\/nu14153170","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T21:01:24Z","timestamp":1659387684000},"page":"3170","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Therapeutic Effects of Live Lactobacillus plantarum GKD7 in a Rat Model of Knee Osteoarthritis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7636-1115","authenticated-orcid":false,"given":"Yen-You","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Medicine, China Medical University, Taichung 404333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunny Li-Yun","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Science, China Medical University, Taichung 404333, Taiwan"},{"name":"School of Medicine, China Medical University, Taichung 404333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan-Chi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Education and Research, China Medical University Beigang Hospital, Yunlin 651012, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2917-0578","authenticated-orcid":false,"given":"David","family":"Achudhan","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Science, China Medical University, Taichung 404333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Shan","family":"Tsai","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio Ltd., Taoyuan 325002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Wei","family":"Lin","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio Ltd., Taoyuan 325002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen-Lien","family":"Chen","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio Ltd., Taoyuan 325002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Chu","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, National Taiwan University, Taipei 106617, Taiwan"},{"name":"Department of Food Science, Nutrition and Nutraceutical Biotechnology, Shih Chien University, Taipei 104336, Taiwan"},{"name":"Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Way","family":"Chang","sequence":"additional","affiliation":[{"name":"The Ph.D. Program of Biotechnology and Biomedical Industry, China Medical University, Taichung 404333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Chin","family":"Fong","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, College of Health Care, China Medical University, Taichung 404333, Taiwan"},{"name":"Department of Orthopaedic Surgery, China Medical University Beigang Hospital, Yunlin 651012, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Lin","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Medicine, China Medical University, Taichung 404333, Taiwan"},{"name":"Department of Family Medicine, China Medical University Hsinchu Hospital, Hsinchu 302056, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7113-8352","authenticated-orcid":false,"given":"Chih-Hsin","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, China Medical University, Taichung 404333, Taiwan"},{"name":"Graduate Institute of Biomedical Science, China Medical University, Taichung 404333, Taiwan"},{"name":"School of Medicine, China Medical University, Taichung 404333, Taiwan"},{"name":"Chinese Medicine Research Center, China Medical University, Taichung 404333, Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung 413305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/s41584-019-0221-y","article-title":"Therapeutic options for targeting inflammatory osteoarthritis pain","volume":"15","author":"Conaghan","year":"2019","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1093\/rheumatology\/keu529","article-title":"Ultrasound assessment of response to intra-articular therapy in osteoarthritis of the knee","volume":"54","author":"Keen","year":"2015","journal-title":"Rheumatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1007\/s11357-022-00525-3","article-title":"New imaging tools for mouse models of osteoarthritis","volume":"44","author":"Drevet","year":"2022","journal-title":"GeroScience"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"678575","DOI":"10.3389\/fimmu.2021.678757","article-title":"Synovial Macrophages in Osteoarthritis: The Key to Understanding Pathogenesis?","volume":"12","author":"Thomson","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"595273","DOI":"10.1155\/2015\/595273","article-title":"Study of Osteoarthritis Treatment with Anti-Inflammatory Drugs: Cyclooxygenase-2 Inhibitor and Steroids","volume":"2015","author":"Cho","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1016\/S0140-6736(19)30417-9","article-title":"Osteoarthritis","volume":"393","author":"Hunter","year":"2019","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"114147","DOI":"10.1016\/j.bcp.2020.114147","article-title":"Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective","volume":"180","author":"Bindu","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"561459","DOI":"10.1155\/2014\/561459","article-title":"The Role of Inflammatory and Anti-Inflammatory Cytokines in the Pathogenesis of Osteoarthritis","volume":"2014","author":"Wojdasiewicz","year":"2014","journal-title":"Mediat. Inflamm."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Molnar, V., Mati\u0161i\u0107, V., Kodvanj, I., Bjelica, R., Jele\u010d, Z., Hudetz, D., Rod, E., \u010cukelj, F., Vrdoljak, T., and Vidovi\u0107, D. (2021). Cytokines and Chemokines Involved in Osteoarthritis Pathogenesis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22179208"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1302\/2058-5241.6.200040","article-title":"Autologous protein solution: A promising solution for osteoarthritis?","volume":"6","author":"Razak","year":"2021","journal-title":"EFORT Open Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6093","DOI":"10.3390\/ijms16036093","article-title":"Osteoarthritis in the XXIst Century: Risk Factors and Behaviours that Influence Disease Onset and Progression","volume":"16","author":"Musumeci","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"343","DOI":"10.2165\/11599350-000000000-00000","article-title":"Role of interleukin-1 inhibitors in osteoarthritis: An evidence-based review","volume":"29","author":"Jotanovic","year":"2012","journal-title":"Drugs Aging"},{"key":"ref_13","first-page":"4737","article-title":"TNF-\u03b1 increases the expression of inflammatory factors in synovial fibroblasts by inhibiting the PI3K\/AKT pathway in a rat model of monosodium iodoacetate-induced osteoarthritis","volume":"16","author":"Li","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s12602-019-09577-y","article-title":"Anti-inflammatory and Anti-osteoporotic Potential of Lactobacillus plantarum A41 and L. fermentum SRK414 as Probiotics","volume":"12","author":"Lee","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1016\/j.sjbs.2015.09.022","article-title":"In vitro importance of probiotic Lactobacillus plantarum related to medical field","volume":"23","author":"Arasu","year":"2016","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8156","DOI":"10.1038\/s41598-018-26588-x","article-title":"Anti-inflammatory effects of heat-killed Lactobacillus plantarum L-137 on cardiac and adipose tissue in rats with metabolic syndrome","volume":"8","author":"Uchinaka","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1046\/j.1462-5822.2003.t01-1-00275.x","article-title":"Lactobacillus rhamnosus GG decreases TNF-alpha production in lipopolysaccharide-activated murine macrophages by a contact-independent mechanism","volume":"5","author":"Pena","year":"2003","journal-title":"Cell. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1089\/jir.2016.0057","article-title":"Lactobacillus salivarius Isolated from Patients with Rheumatoid Arthritis Suppresses Collagen-Induced Arthritis and Increases Treg Frequency in Mice","volume":"36","author":"Liu","year":"2016","journal-title":"J. Interferon Cytokine Res. Off. J. Int. Soc. Interferon Cytokine Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Jhun, J., Cho, K.H., Lee, D.H., Kwon, J.Y., Woo, J.S., Kim, J., Na, H.S., Park, S.H., Kim, S.J., and Cho, M.L. (2021). Oral Administration of Lactobacillus rhamnosus Ameliorates the Progression of Osteoarthritis by Inhibiting Joint Pain and Inflammation. Cells, 10.","DOI":"10.21203\/rs.3.rs-220741\/v1"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"812596","DOI":"10.3389\/fcimb.2022.812596","article-title":"Association Between Gut Microbiota and Osteoarthritis: A Review of Evidence for Potential Mechanisms and Therapeutics","volume":"12","author":"Wei","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_21","first-page":"14","article-title":"Crosstalk between gut microbiota and osteoarthritis: A critical view","volume":"68","author":"Korotkyia","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"222","DOI":"10.3892\/etm.2021.9653","article-title":"Effects of Streptococcus thermophilus on anterior cruciate ligament transection-induced early osteoarthritis in rats","volume":"21","author":"Lin","year":"2021","journal-title":"Exp. Ther. Med."},{"key":"ref_23","first-page":"9","article-title":"A Screening of Complex Probiotics for Reducing Aspirin-Induced Gastric Injury in Mice","volume":"11","year":"2022","journal-title":"Hans J. Food Nutr. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6988","DOI":"10.1002\/jcp.30361","article-title":"miR-144-3p ameliorates the progression of osteoarthritis by targeting IL-1beta: Potential therapeutic implications","volume":"236","author":"Lin","year":"2021","journal-title":"J. Cell Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e14108","DOI":"10.1111\/jfbc.14108","article-title":"Cordycerebroside A inhibits ICAM-1-dependent M1 monocyte adhesion to osteoarthritis synovial fibroblasts","volume":"46","author":"Lee","year":"2022","journal-title":"J. Food Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5367494","DOI":"10.1155\/2022\/5367494","article-title":"The Traditional Chinese Medicine \u201cHu-Qian-Wan\u201d Attenuates Osteoarthritis-Induced Signs and Symptoms in an Experimental Rat Model of Knee Osteoarthritis","volume":"2022","author":"Hou","year":"2022","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Wang, Y.-H., Kuo, S.-J., Liu, S.-C., Wang, S.-W., Tsai, C.-H., Fong, Y.-C., and Tang, C.-H. (2020). Apelin Affects the Progression of Osteoarthritis by Regulating VEGF-Dependent Angiogenesis and miR-150-5p Expression in Human Synovial Fibroblasts. Cells, 9.","DOI":"10.3390\/cells9030594"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chen, W.-C., Lin, C.-Y., Kuo, S.-J., Liu, S.-C., Lu, Y.-C., Chen, Y.-L., Wang, S.-W., and Tang, C.-H. (2020). Resistin Enhances VCAM-1 Expression and Monocyte Adhesion in Human Osteoarthritis Synovial Fibroblasts by Inhibiting MiR-381 Expression through the PKC, p38, and JNK Signaling Pathways. Cells, 9.","DOI":"10.3390\/cells9061369"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/s41419-022-04656-5","article-title":"Melatonin abolished proinflammatory factor expression and antagonized osteoarthritis progression in vivo","volume":"13","author":"Liu","year":"2022","journal-title":"Cell Death Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9224","DOI":"10.18632\/aging.103195","article-title":"Apelin enhances IL-1\u03b2 expression in human synovial fibroblasts by inhibiting miR-144-3p through the PI3K and ERK pathways","volume":"12","author":"Chang","year":"2020","journal-title":"Aging"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"104712","DOI":"10.1016\/j.jff.2021.104712","article-title":"Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK\/ERK\/AP-1 signaling","volume":"86","author":"Lee","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_32","first-page":"e14022","article-title":"Antcin K inhibits VEGF-dependent angiogenesis in human rheumatoid arthritis synovial fibroblasts","volume":"46","author":"Achudhan","year":"2021","journal-title":"J. Food Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.joca.2005.07.014","article-title":"Osteoarthritis cartilage histopathology: Grading and staging","volume":"14","author":"Pritzker","year":"2006","journal-title":"Osteoarthr. Cartil."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"S24","DOI":"10.1016\/j.joca.2010.05.030","article-title":"The OARSI histopathology initiative\u2013recommendations for histological assessments of osteoarthritis in the rat","volume":"18","author":"Gerwin","year":"2010","journal-title":"Osteoarthr. Cartil."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wirth, M., Jira, D., Ott, A., Piontek, G., and Pickhard, A. (2018). High NOTCH1 mRNA Expression Is Associated with Better Survival in HNSCC. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19030830"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1080\/09540105.2020.1766426","article-title":"Soya-cerebroside reduces interleukin production in human rheumatoid arthritis synovial fibroblasts by inhibiting the ERK, NF-\u03baB and AP-1 signalling pathways","volume":"31","author":"Lee","year":"2020","journal-title":"Food Agric. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1038\/s41584-022-00749-9","article-title":"Synovial inflammation in osteoarthritis progression","volume":"18","author":"Coras","year":"2022","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13075-021-02427-9","article-title":"The gut microbiota in osteoarthritis: Where do we stand and what can we do?","volume":"23","author":"Hao","year":"2021","journal-title":"Arthritis Res. Ther."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Warman, D.J., Jia, H., and Kato, H. (2022). The Potential Roles of Probiotics, Resistant Starch, and Resistant Proteins in Ameliorating Inflammation during Aging (Inflammaging). Nutrients, 14.","DOI":"10.3390\/nu14040747"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"104729","DOI":"10.1016\/j.jff.2021.104729","article-title":"Betulin suppresses TNF-\u03b1 and IL-1\u03b2 production in osteoarthritis synovial fibroblasts by inhibiting the MEK\/ERK\/NF-\u03baB pathway","volume":"86","author":"Su","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.imlet.2018.07.003","article-title":"Lactobacillus acidophilus ameliorates pain and cartilage degradation in experimental osteoarthritis","volume":"203","author":"Lee","year":"2018","journal-title":"Immunol. Lett."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"104039","DOI":"10.1016\/j.jff.2020.104039","article-title":"Lactobacillus M5 prevents osteoarthritis induced by a high-fat diet in mice","volume":"72","author":"Song","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1136\/annrheumdis-2020-218089","article-title":"Microenvironment in subchondral bone: Predominant regulator for the treatment of osteoarthritis","volume":"80","author":"Hu","year":"2020","journal-title":"Ann. Rheum. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41413-021-00147-z","article-title":"Subchondral bone microenvironment in osteoarthritis and pain","volume":"9","author":"Hu","year":"2021","journal-title":"Bone Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s10067-009-1352-3","article-title":"Osteoarthritis: Can anti-cytokine therapy play a role in treatment?","volume":"29","author":"Calich","year":"2010","journal-title":"Clin. Rheumatol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.cyto.2014.06.019","article-title":"Osteoarthritis joint pain: The cytokine connection","volume":"70","author":"Miller","year":"2014","journal-title":"Cytokine"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"951","DOI":"10.2147\/TCRM.S139814","article-title":"Mechanism and therapeutic effectiveness of nerve growth factor in osteoarthritis pain","volume":"13","author":"Shang","year":"2017","journal-title":"Ther. Clin. Risk Manag."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"570639","DOI":"10.1155\/2016\/5706359","article-title":"Nerve Growth Factor Regulation by TNF-\u03b1and IL-1\u03b2in Synovial Macrophages and Fibroblasts in Osteoarthritic Mice","volume":"2016","author":"Takano","year":"2016","journal-title":"J. Immunol. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1016\/j.joca.2015.04.012","article-title":"Knee osteoarthritis patients with severe nocturnal pain have altered proximal tibial subchondral bone mineral density","volume":"23","author":"Burnett","year":"2015","journal-title":"Osteoarthr. Cartil."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/15\/3170\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:39Z","timestamp":1760140839000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/15\/3170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,1]]},"references-count":49,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["nu14153170"],"URL":"https:\/\/doi.org\/10.3390\/nu14153170","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,1]]}}}