{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T03:14:13Z","timestamp":1785554053128,"version":"3.56.0"},"reference-count":66,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T00:00:00Z","timestamp":1662336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>\u03b3-Conglutin (\u03b3-C) is the glycoprotein from the edible seed L. albus, studied for long time for its postprandial glycaemic regulating action. It still lacks clear information on what could happen at the meeting point between the protein and the organism: the intestinal barrier. We compared an in vitro system involving Caco-2 and IPEC-J2 cells with an ex vivo system using pig ileum and jejunum segments to study \u03b3-C transport from the apical to the basolateral compartment, and its effects on the D-glucose uptake and glucose transporters protein expression. Finally, we studied its potential in modulating glucose metabolism by assessing the possible inhibition of \u03b1-amylase and \u03b1-glucosidase. RP-HPLC analyses showed that \u03b3-C may be transported to the basolateral side in the in vitro system but not in the pig intestines. \u03b3-C was also able to promote a decrease in glucose uptake in both cells and jejunum independently from the expression of the SGLT1 and GLUT2 transporters.<\/jats:p>","DOI":"10.3390\/nu14173666","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T01:51:57Z","timestamp":1662515517000},"page":"3666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Lupinus albus \u03b3-Conglutin: New Findings about Its Action at the Intestinal Barrier and a Critical Analysis of the State of the Art on Its Postprandial Glycaemic Regulating Activity"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8791-6219","authenticated-orcid":false,"given":"Giuditta C.","family":"Heinzl","sequence":"first","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences, Universit\u00e0 degli Studi di Milano, 20133 Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3317-4384","authenticated-orcid":false,"given":"Marco","family":"Tretola","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"},{"name":"Institute for Livestock Sciences, Agroscope, 1725 Posieux, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7787-9645","authenticated-orcid":false,"given":"Stefano","family":"De Benedetti","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences, Universit\u00e0 degli Studi di Milano, 20133 Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Silacci","sequence":"additional","affiliation":[{"name":"Institute for Livestock Sciences, Agroscope, 1725 Posieux, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1970-0122","authenticated-orcid":false,"given":"Alessio","family":"Scarafoni","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences, Universit\u00e0 degli Studi di Milano, 20133 Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1080\/10408398.2013.814044","article-title":"1-Nutritional, Health, and Technological Functionality of Lupin Flour Addition to Bread and Other Baked Products: Benefits and Challenges","volume":"56","author":"Villarino","year":"2016","journal-title":"Crit. 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