{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:13:35Z","timestamp":1760231615644,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T00:00:00Z","timestamp":1663977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K10909","19H04022"],"award-info":[{"award-number":["18K10909","19H04022"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Ministry of Education, Science, and Culture of Japan","doi-asserted-by":"publisher","award":["18K10909","19H04022"],"award-info":[{"award-number":["18K10909","19H04022"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>This research examined the effects of single-dose molecular hydrogen (H2) supplements on acid-base status and local muscle deoxygenation during rest, high-intensity intermittent training (HIIT) performance, and recovery. Ten healthy, trained subjects in a randomized, double-blind, crossover design received H2-rich calcium powder (HCP) (1500 mg, containing 2.544 \u03bcg of H2) or H2-depleted placebo (1500 mg) supplements 1 h pre-exercise. They performed six bouts of 7 s all-out pedaling (HIIT) at 7.5% of body weight separated by 40 s pedaling intervals, followed by a recovery period. Blood gases\u2019 pH, PCO2, and HCO3\u2212 concentrations were measured at rest. Muscle deoxygenation (deoxy[Hb + Mb]) and tissue O2 saturation (StO2) were determined via time-resolved near-infrared spectroscopy in the vastus lateralis (VL) and rectus femoris (RF) muscles from rest to recovery. At rest, the HCP group had significantly higher PCO2 and HCO3\u2212 concentrations and a slight tendency toward acidosis. During exercise, the first HIIT bout\u2019s peak power was significantly higher in HCP (839\u2009 \u00b1 \u2009112 W) vs. Placebo (816\u2009 \u00b1 \u2009108 W, p = 0.001), and HCP had a notable effect on significantly increased deoxy[Hb + Mb] concentration during HIIT exercise, despite no differences in heart rate response. The HCP group showed significantly greater O2 extraction in VL and microvascular (Hb) volume in RF during HIIT exercise. The HIIT exercise provided significantly improved blood flow and muscle reoxygenation rates in both the RF and VL during passive recovery compared to rest in all groups. The HCP supplement might exert ergogenic effects on high-intensity exercise and prove advantageous for improving anaerobic HIIT exercise performance.<\/jats:p>","DOI":"10.3390\/nu14193974","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T03:34:17Z","timestamp":1664163257000},"page":"3974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Acute Effects of a Single Dose of Molecular Hydrogen Supplements on Responses to Ergogenic Adjustments during High-Intensity Intermittent Exercise in Humans"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3119-2079","authenticated-orcid":false,"given":"Ahad Abdulkarim D.","family":"Alharbi","sequence":"first","affiliation":[{"name":"Graduate School of Health and Sports Science, Doshisha University, Kyoto 610-0396, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Iwamoto","sequence":"additional","affiliation":[{"name":"Graduate School of Health and Sports Science, Doshisha University, Kyoto 610-0396, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5510-3950","authenticated-orcid":false,"given":"Naoyuki","family":"Ebine","sequence":"additional","affiliation":[{"name":"Graduate School of Health and Sports Science, Doshisha University, Kyoto 610-0396, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Nakae","sequence":"additional","affiliation":[{"name":"Human Augmentation Research Center, National Institute of Advanced Industrial Science and Technology, Kashiwa II Campus, The University of Tokyo, Chiba 277-0882, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Hojo","sequence":"additional","affiliation":[{"name":"Graduate School of Health and Sports Science, Doshisha University, Kyoto 610-0396, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Fukuoka","sequence":"additional","affiliation":[{"name":"Graduate School of Health and Sports Science, Doshisha University, Kyoto 610-0396, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"971","DOI":"10.3109\/10715762.2010.500328","article-title":"Recent advances in hydrogen research as a therapeutic medical gas","volume":"44","author":"Huang","year":"2010","journal-title":"Free Radic. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"789507","DOI":"10.3389\/fphys.2021.789507","article-title":"Hydrogen, a novel therapeutic molecule, regulates oxidative stress, inflammation, and apoptosis","volume":"12","author":"Tian","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nm1577","article-title":"Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals","volume":"13","author":"Ohsawa","year":"2007","journal-title":"Nat. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2328768","DOI":"10.1155\/2020\/2328768","article-title":"Application of molecular hydrogen as a novel antioxidant in sports science","volume":"2020","author":"Kawamura","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pharmthera.2014.04.006","article-title":"Molecular hydrogen as a preventive and therapeutic medical gas: Initiation, development and potential of hydrogen medicine","volume":"144","author":"Ohta","year":"2014","journal-title":"Pharmacol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1055\/s-0034-1395509","article-title":"Molecular hydrogen in sports medicine: New therapeutic perspectives","volume":"36","author":"Ostojic","year":"2014","journal-title":"Int. J. Sports Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Alharbi, A.A.D., Ebine, N., Nakae, S., Hojo, T., and Fukuoka, Y. (2021). Application of molecular hydrogen as an antioxidant in responses to ventilatory and ergogenic adjustments during incremental exercise in humans. Nutrients, 13.","DOI":"10.3390\/nu13020459"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1152\/japplphysiol.01216.2010","article-title":"The relationship between muscle deoxygenation and activation in different muscles of the quadriceps during cycle ramp exercise","volume":"111","author":"Chin","year":"2011","journal-title":"J. Appl. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1152\/japplphysiol.00574.2015","article-title":"Muscle deoxygenation in the quadriceps during ramp incremental cycling: Deep vs. superficial heterogeneity","volume":"119","author":"Okushima","year":"2015","journal-title":"J. Appl. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"686","DOI":"10.3389\/fphys.2017.00686","article-title":"Quadriceps muscles O2 extraction and EMG breakpoints during a ramp incremental test","volume":"8","author":"Iannetta","year":"2017","journal-title":"Front. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jsams.2020.09.011","article-title":"Sodium bicarbonate improves sprint performance in endurance cycling","volume":"24","author":"Dalle","year":"2021","journal-title":"J. Sci. Med. Sport"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12970-021-00410-y","article-title":"Effect of sodium bicarbonate contribution on energy metabolism during exercise: A systematic review and meta-analysis","volume":"18","author":"Calvo","year":"2021","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s12970-019-0285-8","article-title":"Effect of sodium bicarbonate ingestion during 6\u2009weeks of HIIT on anaerobic performance of college students","volume":"16","author":"Wang","year":"2019","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"365","DOI":"10.3389\/fphys.2012.00365","article-title":"PCO2 and pH regulation of cerebral blood flow","volume":"3","author":"Yoon","year":"2012","journal-title":"Front. Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1113\/EP086249","article-title":"Regulation of cerebral blood flow and metabolism during exercise","volume":"102","author":"Smith","year":"2017","journal-title":"Exp. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e12430","DOI":"10.14814\/phy2.12430","article-title":"Impaired cerebral blood flow and oxygenation during exercise in type 2 diabetic patients","volume":"3","author":"Kim","year":"2015","journal-title":"Physiol. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"789053","DOI":"10.3389\/fnhum.2021.789053","article-title":"The exercising brain: An overlooked factor limiting the tolerance to physical exertion in major cardiorespiratory diseases?","volume":"15","author":"Marillier","year":"2022","journal-title":"Front. Hum. Neurosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1007\/s00421-010-1792-0","article-title":"Effect of caffeine ingestion after creatine supplementation on intermittent high-intensity sprint performance","volume":"111","author":"Lee","year":"2011","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s00421-015-3296-4","article-title":"Influence of beetroot juice supplementation on intermittent exercise performance","volume":"116","author":"Wylie","year":"2016","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1113\/expphysiol.2013.073270","article-title":"Slowed oxygen uptake kinetics in hypoxia correlate with the transient peak and reduced spatial distribution of absolute skeletal muscle deoxygenation","volume":"98","author":"Bowen","year":"2013","journal-title":"Exp. Physiol."},{"key":"ref_21","unstructured":"Cohen, J. (1988). Statistical Power Analysis for the Behavioral Sciences, Lawrence Erlbaum. [2nd ed.]."},{"key":"ref_22","unstructured":"Rowntree, D. (1981). Statistics without Tears: A Primer for Non-Mathematicians, Charles Scribner\u2019s Sons."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1177\/011542650802300176","article-title":"Biostatistics Primer: Part 2","volume":"23","author":"Overholser","year":"2008","journal-title":"Nutr. Clin. Pract."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5451","DOI":"10.1113\/jphysiol.2009.178053","article-title":"Progressive argumentation and ventilatory long-term facilitation are enhanced in sleep apnea patients and are mitigated by antioxidant administration","volume":"587","author":"Lee","year":"2009","journal-title":"J. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1113\/expphysiol.2008.045153","article-title":"Intermittent hypoxia and respiratory plasticity in humans and other animals: Does exposure to intermittent hypoxia promote or mitigate sleep apnea?","volume":"94","author":"Mateika","year":"2009","journal-title":"Exp. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Fan, J.L., and Kayser, B. (2013). The effect of adding CO2 to hypoxic inspired gas on cerebral blood flow velocity and breathing during incremental exercise. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0081130"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.resp.2012.07.014","article-title":"Regional cerebral blood flow distribution during exercise: Influence of oxygen","volume":"184","author":"Smith","year":"2012","journal-title":"Respir. Physiol. Neurobiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1152\/ajpheart.01104.2007","article-title":"Cerebrovascular responses to incremental exercise during hypobaric hypoxia: Effect of oxygenation on maximal performance","volume":"94","author":"Subudhi","year":"2008","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e12432","DOI":"10.14814\/phy2.12432","article-title":"Reduction of VO2 slow component by priming exercise: Novel mechanistic insights from time-resolved near-infrared spectroscopy","volume":"3","author":"Fukuoka","year":"2015","journal-title":"Physiol. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1152\/japplphysiol.00745.2009","article-title":"Comparison of exogenous adenosine and voluntary exercise on human skeletal muscle perfusion and perfusion heterogeneity","volume":"108","author":"Heinonen","year":"2010","journal-title":"J. Appl. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s004210000267","article-title":"Muscle blood flow and flow heterogeneity during exercise studied with positron emission tomography in humans","volume":"83","author":"Kalliokoski","year":"2000","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1152\/japplphysiol.00731.2002","article-title":"Muscle oxygen extraction and perfusion heterogeneity during continuous and intermittent static exercise","volume":"94","author":"Kalliokoski","year":"2003","journal-title":"J. Appl. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/j.1748-1716.1983.tb07185.x","article-title":"Distribution of different fibre types in human skeletal muscles. 2. A study of cross-sections of whole m. vastus lateralis","volume":"117","author":"Lexell","year":"1983","journal-title":"Acta Physiol. Scand."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Korthuis, R.J. (2011). Skeletal Muscle Circulation, Morgan & Claypool Life Sciences.","DOI":"10.4199\/C00035ED1V01Y201106ISP023"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"St\u00f6cker, F., Von Oldershausen, C., Paternoster, F.K., Schulz, T., and Oberhoffer, R. (2016). Relationship of post-exercise muscle oxygenation and duration of cycling exercise. BMC Sports Sci. Med. Rehabil., 14.","DOI":"10.1186\/s13102-016-0036-y"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/19\/3974\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:38:55Z","timestamp":1760143135000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/19\/3974"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,24]]},"references-count":35,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["nu14193974"],"URL":"https:\/\/doi.org\/10.3390\/nu14193974","relation":{},"ISSN":["2072-6643"],"issn-type":[{"type":"electronic","value":"2072-6643"}],"subject":[],"published":{"date-parts":[[2022,9,24]]}}}