{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:34:18Z","timestamp":1790836458594,"version":"4.1.0"},"reference-count":18,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005754","name":"Gothenburg Medical Association, Sahlgrenska University Hospital","doi-asserted-by":"publisher","award":["ALFGBG-673941"],"award-info":[{"award-number":["ALFGBG-673941"]}],"id":[{"id":"10.13039\/501100005754","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005754","name":"Gothenburg Medical Association, Sahlgrenska University Hospital","doi-asserted-by":"publisher","award":["ALFGBG721551"],"award-info":[{"award-number":["ALFGBG721551"]}],"id":[{"id":"10.13039\/501100005754","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005754","name":"Gothenburg Medical Association, Sahlgrenska University Hospital","doi-asserted-by":"publisher","award":["MRC_MC_UU_12012\/5"],"award-info":[{"award-number":["MRC_MC_UU_12012\/5"]}],"id":[{"id":"10.13039\/501100005754","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008583","name":"Erik and Lily Philipsons Memorial","doi-asserted-by":"publisher","award":["ALFGBG-673941"],"award-info":[{"award-number":["ALFGBG-673941"]}],"id":[{"id":"10.13039\/501100008583","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008583","name":"Erik and Lily Philipsons Memorial","doi-asserted-by":"publisher","award":["ALFGBG721551"],"award-info":[{"award-number":["ALFGBG721551"]}],"id":[{"id":"10.13039\/501100008583","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008583","name":"Erik and Lily Philipsons Memorial","doi-asserted-by":"publisher","award":["MRC_MC_UU_12012\/5"],"award-info":[{"award-number":["MRC_MC_UU_12012\/5"]}],"id":[{"id":"10.13039\/501100008583","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background and aims: The conditions for jejunal glucose absorption in healthy subjects have not been thoroughly studied. In this study we investigated differences in the jejunal villi enlargement factor, as well as ultrastructural aspects of the surface enterocytes and mitochondria, comparing 2 weeks of high-carbohydrate (HCD) versus high-fat diets (HFD). We also measured the ketogenesis rate-limiting enzyme 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS2) in relation to jejunal mitochondria. Methods: A single-centre, randomized, unblinded crossover study in 15 healthy volunteers ingesting strictly controlled equicaloric diets (either HCD or HFD), with 60% energy from the respective source. An enteroscopy was carried out after 2 weeks of each diet and jejunal mucosal biopsies were acquired. Conventional histology, immunofluorescent staining, transmission electron microscopy and confocal microscopy were used. Results: The villi did not demonstrate any change in the epithelial enlargement factor. Despite an increased mitosis, there were no changes in apoptotic indices. However, the ultrastructural analysis demonstrated a significant increase in the enlargement factor at the bases of the villi. The mitochondria demonstrated increased amounts of cristae after the HFD. The confocal microscopy revealed increased HMGCS2 per mitochondrial marker at the top of the villi after the HFD compared to the HCD. Conclusion: There is a morphometric adaption in the jejunal mucosa following the 2-week diets, not only on a histological level, but rather on the ultrastructural level. This study supports the notion that mitochondrial HMGCS2 is regulated by the fat content of the diet and is involved in the expression of monosaccharide transporters.<\/jats:p>","DOI":"10.3390\/nu14194123","type":"journal-article","created":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T01:21:07Z","timestamp":1665364867000},"page":"4123","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Morphological Adaptation in the Jejunal Mucosa after Iso-Caloric High-Fat versus High-Carbohydrate Diets in Healthy Volunteers: Data from a Randomized Crossover Study"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7514-7435","authenticated-orcid":false,"given":"Anna","family":"Casselbrant","sequence":"first","affiliation":[{"name":"Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8668-3196","authenticated-orcid":false,"given":"Ville","family":"Wallenius","sequence":"additional","affiliation":[{"name":"Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden"},{"name":"Department of Surgery, Region V\u00e4stra G\u00f6taland, Sahlgrenska University Hospital, SE-41345 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Elebring","sequence":"additional","affiliation":[{"name":"Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7347-3085","authenticated-orcid":false,"given":"Hanns-Ulrich","family":"Marschall","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengt R.","family":"Johansson","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert F.","family":"Helander","sequence":"additional","affiliation":[{"name":"Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0736-3034","authenticated-orcid":false,"given":"Lars","family":"F\u00e4ndriks","sequence":"additional","affiliation":[{"name":"Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden"},{"name":"Department of Surgery, Region V\u00e4stra G\u00f6taland, Sahlgrenska University Hospital, SE-41345 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"681","DOI":"10.3109\/00365521.2014.898326","article-title":"Surface area of the digestive tract\u2014Revisited","volume":"49","author":"Helander","year":"2014","journal-title":"Scand. 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