{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:12:45Z","timestamp":1787854365936,"version":"build-2784847793"},"reference-count":42,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T00:00:00Z","timestamp":1665187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Young Teachers Research Innovation Capacity Enhancement Program of Beijing Universityof Agricultural","award":["QJKC2022045"],"award-info":[{"award-number":["QJKC2022045"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Alcohol can cause injury and lead to an inflammatory response in the liver. The NF-\u03baB\/AMPK signaling pathway plays a vital role in regulating intracellular inflammatory cytokine levels. In this study, corn oligopeptides (CPs), as the research objects, were obtained from corn gluten meal, and their regulation of the activation of the Kupffer cell NF-\u03baB\/AMPK signal pathway induced by LPS was investigated. Results showed that ALT, AST, and inflammatory cytokines in mice serum after the administration of CPs at 0.2, 0.4, and 0.8 g\/kg of body weight displayed a distinct (p &lt; 0.05) reduction. On the other hand, the CPs also inhibited the expression of recognized receptor CD14 and TLR4, down-regulated P-JNK, P-ERK, and P-p-38, and thus inhibited inflammatory cytokine levels in Kupffer cells (KCs). Furthermore, four kinds of dipeptides with a leucine residue at the C-terminus that might exhibit down-regulated inflammatory cytokines in the NF-\u03baB\/AMPK signaling pathway functions were detected using HPLC-MS\/MS. These results indicated that CPs have a potential application value in acute alcoholic liver disease.<\/jats:p>","DOI":"10.3390\/nu14194194","type":"journal-article","created":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T01:21:07Z","timestamp":1665364867000},"page":"4194","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The Protective Effects of Corn Oligopeptides on Acute Alcoholic Liver Disease by Inhibiting the Activation of Kupffer Cells NF-\u03baB\/AMPK Signal Pathway"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0943-1793","authenticated-orcid":false,"given":"Ying","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6201-2232","authenticated-orcid":false,"given":"Mingliang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9510-0113","authenticated-orcid":false,"given":"Meiling","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0123-228X","authenticated-orcid":false,"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Academy of National Food and Strategic Reserves Administration, Beijing 100037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangning","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22062","DOI":"10.3390\/ijms160922062","article-title":"Inhibition of Hepatocyte Apoptosis: An Important Mechanism of Corn Peptides Attenuating Liver Injury Induced by Ethanol","volume":"16","author":"Ma","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1002\/hep.23258","article-title":"Alcoholic liver disease","volume":"51","author":"Dasarathy","year":"2010","journal-title":"Hepatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2997","DOI":"10.1111\/1750-3841.13953","article-title":"Hepatoprotective Effect of Albumin Peptides from Corn Germ Meal on Chronic Alcohol-Induced Liver Injury in Mice","volume":"82","author":"Yu","year":"2017","journal-title":"J. Food Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1016\/j.fct.2012.03.083","article-title":"Corn oligopeptides protect against early alcoholic liver injury in rats","volume":"50","author":"Zhang","year":"2012","journal-title":"Food Chem. Toxicol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S38","DOI":"10.1016\/j.jhep.2015.03.006","article-title":"Trends in the management and burden of alcoholic liver disease","volume":"62","author":"Mathurin","year":"2015","journal-title":"J. Hepatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.jhep.2008.10.010","article-title":"Epidemiology of non-alcoholic fatty liver disease in China","volume":"50","author":"Fan","year":"2009","journal-title":"J. Hepatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/jgh.12036","article-title":"Epidemiology of alcoholic and nonalcoholic fatty liver disease in China","volume":"28","author":"Fan","year":"2013","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.jhep.2008.06.018","article-title":"Increased overall mortality and liver-related mortality in non-alcoholic fatty liver disease","volume":"49","author":"Ong","year":"2008","journal-title":"J. Hepatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.atherosclerosis.2006.08.021","article-title":"Non-alcoholic fatty liver disease and increased risk of cardiovascular disease","volume":"191","author":"Targher","year":"2007","journal-title":"Atherosclerosis"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0168-8278(00)80420-1","article-title":"Alcoholic liver disease: New insights in pathogenesis lead to new treatments","volume":"32","author":"Lieber","year":"2000","journal-title":"J. Hepatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e3152","DOI":"10.1038\/cddis.2017.532","article-title":"Inhibition of Caspase-8 does not protect from alcohol-induced liver apoptosis but alleviates alcoholic hepatic steatosis in mice","volume":"8","author":"Hao","year":"2017","journal-title":"Cell Death Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.1001\/jama.2020.9184","article-title":"Prescription Fill Patterns for Commonly Used Drugs During the COVID-19 Pandemic in the United States","volume":"323","author":"Vaduganathan","year":"2020","journal-title":"JAMA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1111\/j.1530-0277.2010.01399.x","article-title":"Molecular mechanisms of alcoholic liver disease: Innate immunity and cytokines","volume":"35","author":"Miller","year":"2011","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/s41572-018-0014-7","article-title":"Alcoholic liver disease","volume":"4","author":"Seitz","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.jep.2008.02.023","article-title":"Hepatoprotective effects of Ganoderma lucidum peptides against D-galactosamine-induced liver injury in mice","volume":"117","author":"Shi","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"265","DOI":"10.18388\/abp.2012_2149","article-title":"Protective effects of cassia seed ethanol extract against carbon tetrachloride-induced liver injury in mice","volume":"59","author":"Xie","year":"2012","journal-title":"Acta Biochim. Pol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Liu, W.L., Chen, X.W., Li, H., Zhang, J., An, J.L., and Liu, X.Q. (2022). Anti-Inflammatory Function of Plant-Derived Bioactive Peptides: A Review. Foods, 11.","DOI":"10.3390\/foods11152361"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.foodchem.2015.04.092","article-title":"Antioxidant peptides from corn gluten meal: Orthogonal design evaluation","volume":"187","author":"Zhou","year":"2015","journal-title":"Food Chem"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"124931","DOI":"10.1016\/j.foodchem.2019.05.205","article-title":"Changes in peptidomes and Fischer ratios of corn-derived oligopeptides depending on enzyme hydrolysis approaches","volume":"297","author":"Wang","year":"2019","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jprot.2014.03.044","article-title":"Preventive and therapeutic potential of peptides from cereals against cancer","volume":"111","author":"Winkler","year":"2014","journal-title":"J. Proteom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3264","DOI":"10.1002\/jsfa.6170","article-title":"Optimisation of antioxidant peptide preparation from corn gluten meal","volume":"93","author":"Zhuang","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8873","DOI":"10.1016\/j.biortech.2008.04.056","article-title":"Enzymatic preparation of immunomodulating hydrolysates from soy proteins","volume":"99","author":"Kong","year":"2008","journal-title":"Bioresour. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e13055","DOI":"10.1111\/jfbc.13055","article-title":"Hypoglycemic effects and biochemical mechanisms of Pea oligopeptide on high-fat diet and streptozotocin-induced diabetic mice","volume":"43","author":"Wei","year":"2019","journal-title":"J. Food Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/j.1440-1711.2005.01323.x","article-title":"Reactive oxygen species are essential mediators in antigen presentation by Kupffer cells","volume":"83","author":"Maemura","year":"2005","journal-title":"Immunol. Cell Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.carbpol.2019.01.044","article-title":"Structural characterization and in vitro hypoglyce-mic activity of a glucan from Euryale ferox Salisb. seeds","volume":"209","author":"Zhang","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4234","DOI":"10.1002\/jssc.201600554","article-title":"Purification and identification of corn peptides that facilitate alcohol metabolism by semi-preparative high-performance liquid chromatography and nano liquid chromatography with electrospray ionization tandem mass spectrometry","volume":"39","author":"Ma","year":"2016","journal-title":"J. Sep. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3389\/fchem.2017.00044","article-title":"Anti-cancer activity of maize bioactive peptides","volume":"5","year":"2017","journal-title":"Front. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1021\/acs.jafc.7b04562","article-title":"Identification of new anti-inflammatory peptides from zein hydrolysate after simulated gastrointestinal digestion and transport in caco-2 cells","volume":"66","author":"Liang","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kany, S., Vollrath, J.T., and Relja, B. (2019). Cytokines in Inflammatory Disease. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20236008"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.cld.2016.02.004","article-title":"Pathogenesis of Alcoholic Liver Disease","volume":"20","author":"Dunn","year":"2016","journal-title":"Clin. Liver Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1159\/000444545","article-title":"Pathogenesis and Management of Alcoholic Liver Disease","volume":"34","author":"Farooq","year":"2016","journal-title":"Dig. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"173","DOI":"10.5009\/gnl16477","article-title":"Pathophysiology and Management of Alcoholic Liver Disease: Update 2016","volume":"11","author":"Stickel","year":"2017","journal-title":"Gut Liver"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/09540105.2015.1079597","article-title":"In vitro antioxidant and protective effects of corn peptides on ethanol-induced damage in HepG2 cells","volume":"27","author":"She","year":"2016","journal-title":"Food Agric. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"332","DOI":"10.4254\/wjh.v12.i7.332","article-title":"Oxidative stress in alcohol-related liver disease","volume":"12","author":"Tan","year":"2020","journal-title":"World J. Hepatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.3945\/jn.113.174375","article-title":"Oral branched-chain amino acids have a beneficial effect on manifestations of hepatic encephalopathy in a systematic review with meta-analyses of randomized controlled trials","volume":"143","author":"Gluud","year":"2013","journal-title":"J. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"G566","DOI":"10.1152\/ajpgi.00231.2017","article-title":"A specific amino acid formula prevents alcoholic liver disease in rodents","volume":"314","author":"Tedesco","year":"2018","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"44612","DOI":"10.1038\/srep44612","article-title":"Effect of modulation of PPAR-gamma activity on Kupffer cells M1\/M2 polarization in the development of non-alcoholic fatty liver disease","volume":"7","author":"Luo","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.molimm.2017.02.018","article-title":"The role of Kupffer cells in hepatic diseases","volume":"85","author":"Li","year":"2017","journal-title":"Mol. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"56","DOI":"10.3389\/fphys.2012.00056","article-title":"Functional role of monocytes and macrophages for the inflammatory response in acute liver injury","volume":"3","author":"Zimmermann","year":"2012","journal-title":"Front. Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1016\/j.jhep.2017.02.026","article-title":"Targeting hepatic macrophages to treat liver diseases","volume":"66","author":"Tacke","year":"2017","journal-title":"J. Hepatol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"363","DOI":"10.3892\/etm.2014.1740","article-title":"Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway","volume":"8","author":"Wu","year":"2014","journal-title":"Exp. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1021\/acs.jafc.9b04961","article-title":"Rice Bran Phenolic Extract Protects against Alcoholic Liver Injury in Mice by Alleviating Intestinal Microbiota Dysbiosis, Barrier Dysfunction, and Liver Inflammation Mediated by the Endotoxin\u2013TLR4\u2013NF-\u03baB Pathway","volume":"68","author":"Xiao","year":"2020","journal-title":"J. Agric. Food Chem."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/19\/4194\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:48:21Z","timestamp":1760143701000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/19\/4194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,8]]},"references-count":42,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["nu14194194"],"URL":"https:\/\/doi.org\/10.3390\/nu14194194","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,8]]}}}