{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:50:11Z","timestamp":1787068211217,"version":"build-2736575974"},"reference-count":68,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T00:00:00Z","timestamp":1667779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Research Program of State Key Laboratory of Food Science and Technology, Nanchang University","award":["SKLF-ZZA-201912"],"award-info":[{"award-number":["SKLF-ZZA-201912"]}]},{"name":"the Research Program of State Key Laboratory of Food Science and Technology, Nanchang University","award":["20221ZDD02001"],"award-info":[{"award-number":["20221ZDD02001"]}]},{"name":"Central Government Guide Local Special Fund Project for Scientific and Technological Development of Jiangxi Province","award":["SKLF-ZZA-201912"],"award-info":[{"award-number":["SKLF-ZZA-201912"]}]},{"name":"Central Government Guide Local Special Fund Project for Scientific and Technological Development of Jiangxi Province","award":["20221ZDD02001"],"award-info":[{"award-number":["20221ZDD02001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The reported association of Moringa oleifera seeds and allergic disease clinically resembling occupational asthma in cosmetic manufacturing workers has resultedin the need to identify such components in the manufacturing process. However, Moringa oleifera leaves from the same plant, an important food ingredient, have limited immunotoxicity data. This study aimed to determine if Moringa oleifera leafproteins (MLP) can elicit allergic responses in BALB\/c mice. The BALB\/c mice were sensitized twice and challenged 10 times to evaluate the potential allergenicityof MLP in vivo. The results showed increased levels of mast cells, total and specific IgE and IgG, severe signs of systemic anaphylaxis, and reduced body temperature compared with controls. The sensitized mice serum observed enhanced levels of histamine and Th-related cytokine release. Compared with the control group, increased levels of interleukins IL-4, IL-9, and IL-17A and enhanced expression and secretion of normal T cells were found in the culture supernatant of splenocytes treated with MLP.This study suggeststhat MLPcanelicit allergic responses; this providesmore comprehensive guidance for identifying new allergen candidates and developing hypoallergenic MLP products.<\/jats:p>","DOI":"10.3390\/nu14214700","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T10:49:51Z","timestamp":1667904591000},"page":"4700","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Potential Allergenicity Response to Moringa oleifera Leaf Proteins in BALB\/c Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"School of Food Science and Technology, Nanchang University, Nanchang 330031, China"},{"name":"Animal Science and Technology Center, Jiangxi University of Traditional Medicine, Nanchang 330004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Animal Science and Technology Center, Jiangxi University of Traditional Medicine, Nanchang 330004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongliang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"School of Food Science and Technology, Nanchang University, Nanchang 330031, China"},{"name":"Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Animal Science and Technology Center, Jiangxi University of Traditional Medicine, Nanchang 330004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2180-297X","authenticated-orcid":false,"given":"Jinyan","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Nanchang University, Nanchang 330031, China"},{"name":"Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9840-940X","authenticated-orcid":false,"given":"Yong","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Nanchang University, Nanchang 330031, China"},{"name":"Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0314-2077","authenticated-orcid":false,"given":"Xuanyi","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Nanchang University, Nanchang 330031, China"},{"name":"Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youbao","family":"Zhong","sequence":"additional","affiliation":[{"name":"Animal Science and Technology Center, Jiangxi University of Traditional Medicine, Nanchang 330004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4845-383X","authenticated-orcid":false,"given":"Hongbing","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.jaip.2015.05.016","article-title":"Occupational asthma to \"the miracle tree\" (Moringa oleifera): First description","volume":"3","author":"Poussel","year":"2015","journal-title":"J. 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