{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T02:50:18Z","timestamp":1785898218997,"version":"3.56.0"},"reference-count":69,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2021YFC2700403"],"award-info":[{"award-number":["2021YFC2700403"]}]},{"name":"National Key R&amp;D Program of China","award":["2018YFC1003203"],"award-info":[{"award-number":["2018YFC1003203"]}]},{"name":"National Key R&amp;D Program of China","award":["2018YFA0800102"],"award-info":[{"award-number":["2018YFA0800102"]}]},{"name":"National Key R&amp;D Program of China","award":["31871452"],"award-info":[{"award-number":["31871452"]}]},{"name":"National Key R&amp;D Program of China","award":["31871249"],"award-info":[{"award-number":["31871249"]}]},{"name":"National Natural Science Foundation of China","award":["2021YFC2700403"],"award-info":[{"award-number":["2021YFC2700403"]}]},{"name":"National Natural Science Foundation of China","award":["2018YFC1003203"],"award-info":[{"award-number":["2018YFC1003203"]}]},{"name":"National Natural Science Foundation of China","award":["2018YFA0800102"],"award-info":[{"award-number":["2018YFA0800102"]}]},{"name":"National Natural Science Foundation of China","award":["31871452"],"award-info":[{"award-number":["31871452"]}]},{"name":"National Natural Science Foundation of China","award":["31871249"],"award-info":[{"award-number":["31871249"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>We perform quantitative studies to investigate the effect of high-calorie diet on Drosophila oogenesis. We use the central composite design (CCD) method to obtain quadratic regression models of body fat and fertility as a function of the concentrations of protein and sucrose, two major macronutrients in Drosophila diet, and treatment duration. Our results reveal complex interactions between sucrose and protein in impacting body fat and fertility when they are considered as an integrated physiological response. We verify the utility of our quantitative modeling approach by experimentally confirming the physiological responses\u2014including increased body fat, reduced fertility, and ovarian insulin insensitivity\u2014expected of a treatment condition identified by our modeling method. Under this treatment condition, we uncover a Drosophila oogenesis phenotype that exhibits an accumulation of immature oocytes and a halt in the production of mature oocytes, a phenotype that bears resemblance to key aspects of the human condition of polycystic ovary syndrome (PCOS). Our analysis of the dynamic progression of different aspects of diet-induced pathophysiology also suggests an order of the onset timing for obesity, ovarian dysfunction, and insulin resistance. Thus, our study documents the utility of quantitative modeling approaches toward understanding the biology of Drosophila female reproduction, in relation to diet-induced obesity and type II diabetes, serving as a potential disease model for human ovarian dysfunction.<\/jats:p>","DOI":"10.3390\/nu14245365","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T07:59:21Z","timestamp":1671436761000},"page":"5365","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Modeling Obesity-Associated Ovarian Dysfunction in Drosophila"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3370-4296","authenticated-orcid":false,"given":"Huanju","family":"Liu","sequence":"first","affiliation":[{"name":"Women\u2019s Hospital and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou 310058, China"},{"name":"Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorder, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4484-6177","authenticated-orcid":false,"given":"Jiajun","family":"Li","sequence":"additional","affiliation":[{"name":"ZJU-UOE Institute, Zhejiang University School of Medicine, Haining 314400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Chang","sequence":"additional","affiliation":[{"name":"Women\u2019s Hospital and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou 310058, China"},{"name":"Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorder, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7114-8078","authenticated-orcid":false,"given":"Feng","family":"He","sequence":"additional","affiliation":[{"name":"Women\u2019s Hospital and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou 310058, China"},{"name":"Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorder, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1609-3294","authenticated-orcid":false,"given":"Jun","family":"Ma","sequence":"additional","affiliation":[{"name":"Women\u2019s Hospital and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou 310058, China"},{"name":"Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorder, Hangzhou 310058, China"},{"name":"Women\u2019s Reproductive Health Research Laboratory of Zhejiang Province, Hangzhou 310006, China"},{"name":"Zhejiang University-University of Toronto Joint Institute of Genetics and Genome Medicine, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1002\/wdev.235","article-title":"Maternal AP determinants in the Drosophila oocyte and embryo","volume":"5","author":"Ma","year":"2016","journal-title":"Wiley Interdiscip. 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