{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:33:54Z","timestamp":1790836434253,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T00:00:00Z","timestamp":1671840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Soci\u00e9t\u00e9 Francophone du Diab\u00e8te","award":["SFD 2020"],"award-info":[{"award-number":["SFD 2020"]}]},{"name":"Soci\u00e9t\u00e9 Francophone du Diab\u00e8te","award":["261863, Bio-NMR"],"award-info":[{"award-number":["261863, Bio-NMR"]}]},{"name":"Access to Research Infrastructures activity in the 7 th Framework Programme of the EC","award":["SFD 2020"],"award-info":[{"award-number":["SFD 2020"]}]},{"name":"Access to Research Infrastructures activity in the 7 th Framework Programme of the EC","award":["261863, Bio-NMR"],"award-info":[{"award-number":["261863, Bio-NMR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Interactions between mitochondria and the endoplasmic reticulum, known as MAMs, are altered in the liver in obesity, which contributes to disruption of the insulin signaling pathway. In addition, the plasma level of glycine is decreased in obesity, and the decrease is strongly correlated with the severity of insulin resistance. Certain nutrients have been shown to regulate MAMs; therefore, we tested whether glycine supplementation could reduce insulin resistance in the liver by promoting MAM integrity. Glycine (5 mM) supported MAM integrity and insulin response in primary rat hepatocytes cultured under control and lipotoxic (palmitate 500 \u00b5M) conditions for 18 h. In contrast, in C57 BL\/6 JOlaHsd mice (male, 6 weeks old) fed a high-fat, high-sucrose diet (HFHS) for 16 weeks, glycine supplementation (300 mg\/kg) in drinking water during the last 6 weeks (HFHS-Gly) did not reverse the deleterious impact of HFHS-feeding on liver MAM integrity. In addition, glycine supplementation worsened fasting glycemia and glycemic response to intraperitoneal pyruvate injection compared to HFHS. The adverse impact of glycine supplementation on hepatic gluconeogenesis was further supported by the higher oxaloacetate\/acetyl-CoA ratio in the liver in HFHS-Gly compared to HFHS. Although glycine improves MAM integrity and insulin signaling in the hepatocyte in vitro, no beneficial effect was found on the overall metabolic profile of HFHS-Gly-fed mice.<\/jats:p>","DOI":"10.3390\/nu15010096","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T07:31:56Z","timestamp":1672126316000},"page":"96","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Glycine Supplementation in Obesity Worsens Glucose Intolerance through Enhanced Liver Gluconeogenesis"],"prefix":"10.3390","volume":"15","author":[{"given":"Ana\u00efs","family":"Alves","sequence":"first","affiliation":[{"name":"CarMeN laboratory, UMR INSERM U1060\/INRAE U1397, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, 69310 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Lamarche","sequence":"additional","affiliation":[{"name":"Laboratory of Fundamental and Applied Bioenergetics, INSERM U1055, Universit\u00e9 Grenoble Alpes, 38400 Saint Martin d\u2019H\u00e8res, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9my","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"CarMeN laboratory, UMR INSERM U1060\/INRAE U1397, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, 69310 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Drevet Mulard","sequence":"additional","affiliation":[{"name":"ICBMS CNRS U5246, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, 69622 Villeurbanne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4276-4493","authenticated-orcid":false,"given":"Arthur","family":"Bassot","sequence":"additional","affiliation":[{"name":"Erika Cosset Team, Cancer Research Centre of Lyon, UMR INSERM U1052\/CNRS 5286, 69008 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6930-6671","authenticated-orcid":false,"given":"St\u00e9phanie","family":"Chanon","sequence":"additional","affiliation":[{"name":"CarMeN laboratory, UMR INSERM U1060\/INRAE U1397, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, 69310 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Loizon","sequence":"additional","affiliation":[{"name":"CarMeN laboratory, UMR INSERM U1060\/INRAE U1397, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, 69310 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudie","family":"Pinteur","sequence":"additional","affiliation":[{"name":"CarMeN laboratory, UMR INSERM U1060\/INRAE U1397, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, 69310 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Maria Nunes de Lira Gomes","family":"Bloise","sequence":"additional","affiliation":[{"name":"Department of Physical Education and Sport Sciences, Laboratory of Nutrition, Physical Activity and Phenotypic Plasticity, Universidade Federal de Pernambuco, UFPE, 55604-000 Vit\u00f3ria de Santo Ant\u00e3o, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murielle","family":"Godet","sequence":"additional","affiliation":[{"name":"CarMeN laboratory, UMR INSERM U1060\/INRAE U1397, Universit\u00e9 Claude Bernard Lyon 1, Universit\u00e9 de Lyon, 69310 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1064-0293","authenticated-orcid":false,"given":"Gilles J. 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