{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T20:44:21Z","timestamp":1783716261169,"version":"3.55.0"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T00:00:00Z","timestamp":1673481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ELSE Nutrition","award":["6971070"],"award-info":[{"award-number":["6971070"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>A controlled-neonatal piglet trial was conducted to evaluate the impact of a plant-based infant formula containing buckwheat and almonds as the main source of protein compared to a commercially available dairy-based formula on the gut health parameters. Two day old piglets were fed either a plant-based or a dairy-based formula until day 21. Gut microbiome, cytokines, growth and metabolism related outcomes, and intestinal morphology were evaluated to determine the safety of the plant-based infant formula. This study reported that the plant-based formula-fed piglets had a similar intestinal microbiota composition relative to the dairy-based formula-fed group. However, differential abundance of specific microbiota species was detected within each diet group in the small and large intestinal regions and fecal samples. Lactobacillus\u00a0delbrueckii, Lactobacillus\u00a0crispatus, and Fusobacterium sp. had higher abundance in the small intestine of plant-based formula-fed piglets compared to the dairy-based group. Bacteroides nordii, Enterococcus sp., Lactobacillus crispatus, Prevotella sp., Ruminococcus lactaris, Bacteroides nordii, Eisenbergiella sp., Lactobacillus crispatus, Prevotella sp., and Akkermansia muciniphila had greater abundance in the large intestine of the plant based diet fed piglets relative to the dairy-based diet group. In the feces, Clostridiales, Bacteroides uniformis, Butyricimonasvirosa, Cloacibacillus porcorum, Clostridium clostridioforme, and Fusobacterium sp. were abundant in dairy-based group relative to the plant-based group. Lachnospiraceae, Clostridium scindens, Lactobacillus coleohominis, and Prevetolla sp. had greater abundance in the feces of the plant-based group in comparison to the dairy-based group. Gut morphology was similar between the plant and the dairy-based formula-fed piglets. Circulatory cytokines, magnesium, triiodothyronine (T3), thyroxine (T4), thyroid stimulating hormone (TSH), vitamin D, vitamin K, and IgE levels were similar among all piglets independent of dietary group. Overall, the present study demonstrated that a plant-based formula with buckwheat and almonds as the primary source of protein can support similar gut microbiota growth and health outcomes compared to a dairy-based infant formula.<\/jats:p>","DOI":"10.3390\/nu15020383","type":"journal-article","created":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T03:11:02Z","timestamp":1673493062000},"page":"383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of a Plant-Based Infant Formula Containing Almonds and Buckwheat on Gut Microbiota Composition, Intestine Morphology, Metabolic and Immune Markers in a Neonatal Piglet Model"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5652-5688","authenticated-orcid":false,"given":"Manoj","family":"Gurung","sequence":"first","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8906-4056","authenticated-orcid":false,"given":"Fernanda","family":"Rosa","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"},{"name":"School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79409, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brooke","family":"Yelvington","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Terry","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quentin D.","family":"Read","sequence":"additional","affiliation":[{"name":"USDA-ARS, Southeast Area, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1789-4587","authenticated-orcid":false,"given":"Brian D.","family":"Piccolo","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Becky","family":"Moody","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Tripp","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Hoy E.","family":"Pittman","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bobby L.","family":"Fay","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talyor J.","family":"Ross","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D.","family":"Sikes","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica B.","family":"Flowers","sequence":"additional","affiliation":[{"name":"Technical Services Envigo, Teklad Diets, Madison, WI 53713, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renee","family":"Fox","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanya","family":"LeRoith","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences & Pathobiology, Virginia Tech, Blacksburg, VA 24061, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachelanne","family":"Talatala","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Bar-Yoseph","sequence":"additional","affiliation":[{"name":"Else Nutrition GH, Tel Aviv-Yafo 6971070, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4289-9109","authenticated-orcid":false,"given":"Laxmi","family":"Yeruva","sequence":"additional","affiliation":[{"name":"USDA-ARS, South East Area, Arkansas Children\u2019s Nutrition Center, Little Rock, AR 72202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1053\/j.gastro.2014.01.058","article-title":"Diet and the intestinal microbiome: Associations, functions, and implications for health and disease","volume":"146","author":"Albenberg","year":"2014","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/s41575-018-0061-2","article-title":"You are what you eat: Diet, health and the gut microbiota","volume":"16","author":"Zmora","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"708472","DOI":"10.3389\/fimmu.2021.708472","article-title":"The Role of Microbiota in Infant Health: From Early Life to Adulthood","volume":"12","author":"Yao","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1136\/gutjnl-2018-317503","article-title":"Role of the microbiome in human development","volume":"68","author":"Knight","year":"2019","journal-title":"Gut"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.aninu.2017.12.004","article-title":"Gastrointestinal tract (gut) health in the young pig","volume":"4","author":"Pluske","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_6","first-page":"207509","article-title":"Immunonutrition in early life: Diet and immune development","volume":"2012","author":"Yaqoob","year":"2012","journal-title":"Clin. Dev. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0091-6749(73)90019-5","article-title":"Development of childhood allergy in infants fed breast, soy, or cow milk","volume":"51","author":"Halpern","year":"1973","journal-title":"J. Allergy Clin. Immunol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1651-2227.1979.tb05024.x","article-title":"Cow\u2019s milk allergy, incidence and pathogenetic role of early exposure to cow\u2019s milk formula","volume":"68","author":"Stintzing","year":"1979","journal-title":"Acta Paediatr Scand."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1111\/all.12630","article-title":"Incidence and natural history of challenge-proven cow\u2019s milk allergy in European children--EuroPrevall birth cohort","volume":"70","author":"Schoemaker","year":"2015","journal-title":"Allergy"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Flom, J.D., and Sicherer, S.H. (2019). Epidemiology of Cow\u2019s Milk Allergy. Nutrients, 11.","DOI":"10.3390\/nu11051051"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1177\/0009922816664512","article-title":"Cow\u2019s Milk Protein Allergy","volume":"55","author":"Mousan","year":"2016","journal-title":"Clin. Pediatr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1097\/WOX.0b013e3181defeb9","article-title":"World Allergy Organization (WAO) Diagnosis and Rationale for Action against Cow\u2019s Milk Allergy (DRACMA) Guidelines","volume":"3","author":"Fiocchi","year":"2010","journal-title":"World Allergy Organ. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1097\/MPG.0000000000002799","article-title":"North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition Position Paper: Plant-based Milks","volume":"71","author":"Merritt","year":"2020","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1542\/peds.2008-0564","article-title":"Use of soy protein-based formulas in infant feeding","volume":"121","author":"Bhatia","year":"2008","journal-title":"Pediatrics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1046\/j.1440-1754.2003.00178.x","article-title":"Soy infant formula and phytoestrogens","volume":"39","author":"Tuohy","year":"2003","journal-title":"J. Paediatr. Child Health"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jpeds.2012.12.067","article-title":"Body fat and bone mineral content of infants fed breast milk, cow\u2019s milk formula, or soy formula during the first year of life","volume":"163","author":"Andres","year":"2013","journal-title":"J. Pediatr."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Rosa, F., Yelvington, B., Terry, N., Tripp, P., Pittman, H.E., Fay, B.L., Ross, T.J., Sikes, J.D., Flowers, J.B., and Bar-Yoseph, F. (2022). Evaluation of the Safety of a Plant-Based Infant Formula Containing Almonds and Buckwheat in a Neonatal Piglet Model. Nutrients, 14.","DOI":"10.3390\/nu14071499"},{"key":"ref_18","unstructured":"Council, N.R. (2012). Nutrient Requirements of Swine: Eleventh Revised Edition, The National Academies Press."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing","volume":"57","year":"1995","journal-title":"J. R. Stat. Soc. Ser. B (Methodol.)"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Section on Breastfeeding, Eidelman, A.I., Schanler, R.J., Johnston, M., Landers, S., Noble, L., Szucs, K., and Viehmann, L. (2012). Breastfeeding and the use of human milk. Pediatrics, 129, e827\u2013e841.","DOI":"10.1542\/peds.2011-3552"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1097\/MOG.0000000000000778","article-title":"Breastfeeding and the developmental origins of mucosal immunity: How human milk shapes the innate and adaptive mucosal immune systems","volume":"37","author":"Dawod","year":"2021","journal-title":"Curr. Opin. Gastroenterol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s40168-015-0104-7","article-title":"The role of breast-feeding in infant immune system: A systems perspective on the intestinal microbiome","volume":"3","author":"Praveen","year":"2015","journal-title":"Microbiome"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1080\/14767058.2018.1506760","article-title":"Changes of intestinal microbiota in early life","volume":"33","author":"Ficara","year":"2020","journal-title":"J. Matern.-Fetal Neonatal Med."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Fabiano, V., Indrio, F., Verduci, E., Calcaterra, V., Pop, T.L., Mari, A., Zuccotti, G.V., Cullu Cokugras, F., Pettoello-Mantovani, M., and Goulet, O. (2021). Term Infant Formulas Influencing Gut Microbiota: An Overview. Nutrients, 13.","DOI":"10.3390\/nu13124200"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"27427","DOI":"10.1038\/srep27427","article-title":"Uncovering the composition of microbial community structure and metagenomics among three gut locations in pigs with distinct fatness","volume":"6","author":"Yang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1111\/jam.13364","article-title":"Probiotic roles of Lactobacillus sp. in swine: Insights from gut microbiota","volume":"122","author":"Valeriano","year":"2017","journal-title":"J. Appl. Microbiol."},{"key":"ref_28","first-page":"6028606","article-title":"Lactobacillus delbrueckii Ameliorates Intestinal Integrity and Antioxidant Ability in Weaned Piglets after a Lipopolysaccharide Challenge","volume":"2020","author":"Chen","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1099\/ijs.0.02873-0","article-title":"Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium","volume":"54","author":"Derrien","year":"2004","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"13329","DOI":"10.1038\/ncomms13329","article-title":"Akkermansia muciniphila mediates negative effects of IFNgamma on glucose metabolism","volume":"7","author":"Greer","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9066","DOI":"10.1073\/pnas.1219451110","article-title":"Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity","volume":"110","author":"Everard","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Amat, S., Lantz, H., Munyaka, P.M., and Willing, B.P. (2020). Prevotella in Pigs: The Positive and Negative Associations with Production and Health. Microorganisms, 8.","DOI":"10.3390\/microorganisms8101584"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s40104-018-0269-6","article-title":"The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition","volume":"9","author":"Guevarra","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40168-015-0091-8","article-title":"Diet shapes the gut microbiome of pigs during nursing and weaning","volume":"3","author":"Frese","year":"2015","journal-title":"Microbiome"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1038\/s41598-022-05707-9","article-title":"Weaning differentially affects the maturation of piglet peripheral blood and jejunal Peyer\u2019s patches","volume":"12","author":"Correa","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/asj.12399","article-title":"Influence of weaning age on the villous height and disaccharidase activities in the porcine small intestine","volume":"87","author":"Tsukahara","year":"2016","journal-title":"Anim. Sci. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1038\/ismej.2017.119","article-title":"Exact sequence variants should replace operational taxonomic units in marker-gene data analysis","volume":"11","author":"Callahan","year":"2017","journal-title":"ISME J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e0019121","DOI":"10.1128\/mSphere.00191-21","article-title":"Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters","volume":"6","author":"Schloss","year":"2021","journal-title":"mSphere"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/2\/383\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:03:57Z","timestamp":1760119437000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/2\/383"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,12]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["nu15020383"],"URL":"https:\/\/doi.org\/10.3390\/nu15020383","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,12]]}}}