{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:24:04Z","timestamp":1791980644885,"version":"4.4.0"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,19]],"date-time":"2023-02-19T00:00:00Z","timestamp":1676764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Indo-U.S. research fellowship grant for women in STEMM (Science, Technology, Engineering, Mathematics, and Medicine), under the collaboration of the Department of Science and Technology, Govt. of India (GOI), Indo-U.S. Science and Technology Forum, Plants for Human Health Institute NCSU","award":["WIS_Internship_2019_01778"],"award-info":[{"award-number":["WIS_Internship_2019_01778"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Food-derived bioactive peptides (BAPs) obtained from edible insect-protein hold multiple activities promising the potential to target complex pathological mechanisms responsible for chronic health conditions such as hypertension development. In this study, enzymatic protein hydrolysates from non-mulberry edible silkworm Antheraea assama (Muga) and Philosomia ricini (Eri) pupae, specifically Alcalase (A. assama) and Papain (P. ricini) hydrolysates obtained after 60 and 240 min, exhibited the highest ACE-inhibitory and antioxidant properties. The hydrolysates\u2019 fractions (&lt;3, 3\u201310 and &gt;10 kDa), specifically Alc_M60min_F3 (\u22643 kDa) and Pap_E240min_F3 (\u22643 kDa), showed the highest antioxidant and ACE-inhibitory activities, respectively. Further RP-HPLC purified sub-fractions F4 and F6 showed the highest ACE inhibition as well as potent anti-oxinflammatory activities in lipopolysaccharide (LPS)-treated endothelial cells. Indeed, F4 and F6 ACE-inhibitory peptide fractions were effective in preventing p65 nuclear translocation after 3 h of LPS stimulation along with the inhibition of p38 MAPK phosphorylation in HUVEC cells. In addition, pretreatment with F4 and F6 ACE-inhibitory peptide fractions significantly prevented the LPS-induced upregulation of COX-2 expression and IL-1\u03b2 secretion, while the expression of NRF2 (nuclear factor erythroid 2-related factor 2)-regulated enzymes such as HO-1 and NQO1 was induced by both peptide fractions. The derived peptides from edible pupae protein hydrolysates have potentialities to be explored as nutritional approaches against hypertension and related cardiovascular diseases.<\/jats:p>","DOI":"10.3390\/nu15041035","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T01:36:37Z","timestamp":1676856997000},"page":"1035","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Evaluation of Anti-Oxinflammatory and ACE-Inhibitory Properties of Protein Hydrolysates Obtained from Edible Non-Mulberry Silkworm Pupae (Antheraea assama and Philosomia ricinii)"],"prefix":"10.3390","volume":"15","author":[{"given":"Preeti","family":"Sarkar","sequence":"first","affiliation":[{"name":"Department of Food Engineering and Technology, School of Engineering, Tezpur University, Napaam 784028, Assam, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5207-068X","authenticated-orcid":false,"given":"Alessandra","family":"Pecorelli","sequence":"additional","affiliation":[{"name":"Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy"},{"name":"Plants for Human Health Institute, NC Research Campus, NC State University, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brittany","family":"Woodby","sequence":"additional","affiliation":[{"name":"Plants for Human Health Institute, NC Research Campus, NC State University, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Pambianchi","sequence":"additional","affiliation":[{"name":"Plants for Human Health Institute, NC Research Campus, NC State University, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9096-0560","authenticated-orcid":false,"given":"Francesca","family":"Ferrara","sequence":"additional","affiliation":[{"name":"Plants for Human Health Institute, NC Research Campus, NC State University, Kannapolis, NC 28081, USA"},{"name":"Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, 44121 Ferrara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1186-6999","authenticated-orcid":false,"given":"Raj Kumar","family":"Duary","sequence":"additional","affiliation":[{"name":"Department of Food Engineering and Technology, School of Engineering, Tezpur University, Napaam 784028, Assam, India"},{"name":"Department of Dairy Science & Food Technology, Institute of Agricultural Sciences, Banaras Hindu University (BHU), Varanasi 221005, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8792-0947","authenticated-orcid":false,"given":"Giuseppe","family":"Valacchi","sequence":"additional","affiliation":[{"name":"Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy"},{"name":"Plants for Human Health Institute, NC Research Campus, NC State University, Kannapolis, NC 28081, USA"},{"name":"Department of Food and Nutrition, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.freeradbiomed.2011.11.002","article-title":"Role of Reactive Oxygen and Nitrogen Species in the Vascular Responses to Inflammation","volume":"52","author":"Granger","year":"2012","journal-title":"Free Radic. 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