{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:01:33Z","timestamp":1760148093679,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"],"award-info":[{"award-number":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"],"award-info":[{"award-number":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"]}]},{"name":"PAIDI I+D+i Junta de Andaluc\u00eda (Consejer\u00eda de Econom\u00eda y Conocimiento)","award":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"],"award-info":[{"award-number":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"]}]},{"name":"PAIDI Actividades de Transferencia 2017 Junta de Andaluc\u00eda (Consejer\u00eda de Econom\u00eda, Conocimiento, Empresas y Universidad)","award":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"],"award-info":[{"award-number":["DI-17-09627","RTC2019-006806-1","P18-RT-3004","AT17_5489_USE"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>A gluten-free diet (GFD) is currently the only treatment available for patients with celiac disease (CD). However, adherence to a GFD can be challenging because gluten is present in many foods. A lifelong follow-up of patients with CD must be performed to promote adherence to a GFD and to identify the appearance of symptoms and the associated diseases. Therefore, the development of tools to analyze gluten exposure in these patients is important. This study proposes the development of the first automatable ELISA to monitor adherence to a GFD through the quantification of urine gluten immunogenic peptides (u-GIP). Seven healthy volunteers without suspicion of CD and 23 patients with CD were monitored as part of this study to optimize, validate, and apply this assay. Non-interference was found in the urine matrix, and the recovery percentage for spiked samples was 81\u2013101%. The u-GIP was stable for up to 16 days when the samples were stored at different temperatures. Overall, 100% of the patients had detectable u-GIP at diagnosis (range of 0.39\u20132.14 ng GIP\/mL), which reduced to 27% after 12 months on a GFD. Therefore, this highly sensitive immunoassay would allow the analysis of u-GIP from a large battery of samples in clinical laboratories of specialized healthcare centers.<\/jats:p>","DOI":"10.3390\/nu15071730","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T10:19:33Z","timestamp":1680257973000},"page":"1730","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the Usefulness of an Automatable Immunoassay for Monitoring Celiac Disease by Quantification of Immunogenic Gluten Peptides in Urine"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9600-8979","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Segura","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7100-846X","authenticated-orcid":false,"given":"\u00c1ngela","family":"Ruiz-Carnicer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irati","family":"Mend\u00eda","sequence":"additional","affiliation":[{"name":"Biomedal S.L., 41900 Camas, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Garz\u00f3n-Benavides","sequence":"additional","affiliation":[{"name":"Digestive Disease Clinical Unit, CIBERehd, Institute of Biomedicine of Seville (IBiS), SeLiver Group, Virgen del Roc\u00edo Hospital\/CSIC\/US, 41013 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1ngeles E.","family":"Pizarro","sequence":"additional","affiliation":[{"name":"Digestive Disease Clinical Unit, CIBERehd, Institute of Biomedicine of Seville (IBiS), SeLiver Group, Virgen del Roc\u00edo Hospital\/CSIC\/US, 41013 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4982-273X","authenticated-orcid":false,"given":"Isabel","family":"Comino","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9235-9496","authenticated-orcid":false,"given":"Carolina","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1177\/2050640619844125","article-title":"European Society for the Study of Coeliac Disease (ESsCD) guideline for coeliac disease and other gluten-related disorders","volume":"7","author":"Volta","year":"2019","journal-title":"United Eur. 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