{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:31:25Z","timestamp":1791549085097,"version":"4.3.4"},"reference-count":52,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T00:00:00Z","timestamp":1683590400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005911","name":"Najran University, Najran, Saudi Arabia","doi-asserted-by":"publisher","award":["NU\/RG\/MRC\/12\/13"],"award-info":[{"award-number":["NU\/RG\/MRC\/12\/13"]}],"id":[{"id":"10.13039\/501100005911","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: The plant Tinospora cordifolia (TC), traditionally known as guduchi or giloy, is used for a number of health conditions as a nutritional supplement and rejuvenation medicine. Its nutritional supplementary products are traditionally recommended for a wide range of health issues, including diabetes, menstruation discomfort, fever, obesity, inflammation, and more. Unfortunately, there has not been extensive research into its effectiveness in treating or managing insulin resistance, lipid and carbohydrate metabolism, hormonal imbalance, and metabolic syndrome-associated polycystic ovary syndrome (PCOS). Methods: Consequently, the present study was designed to induce insulin resistance, dyslipidemia, hormonal abnormality, hyperglycemia, and menstrual disturbance of PCOS using dehydroepiandrosterone (DHEA) in mice and study the effect of oral TC extracts on these factors by using ancient and modern technologies. During the 21-day study, 6 mg\/100 g\/day of DHEA was given to female mice. Levels of glucose, insulin, lipids, and hormones were estimated. In addition to being seen with the naked eye, the morphological and microscopic changes were also observed on histology slides. Results: The study outcomes show that pretreatment with TC preparations significantly improved biochemical and histological abnormalities in female mice. Diestrus phase was only observed in DHEA-treated animals, while cornified epithelial cells were present in TC-treated mice. Pretreatment with TC satva showed significant (p &lt; 0.001) reductions in body weight compared to placebo. Fasting blood glucose, 1-h OGTT, and 2-h OGTT levels were all significantly lower in TC satva- and oil-treated animals in comparison to the disease control group (p &lt; 0.001). Treatment with TC extracts resulted in a normalization of estradiol, progesterone, and testosterone levels (p &lt; 0.05). Treatment with TC extract improved lipid profiles (p &lt; 0.001), LH\/FSH ratios (p &lt; 0.01), fasting insulin levels (p &lt; 0.001), HOMA-IR (p &lt; 0.001), HOMA-Beta (p &lt; 0.001), and QUICKI (p &lt; 0.001). Both macroscopic and microscopic alterations were seen to be restored after TC extract treatment. After being treated with TC satva, oil, and hydroalcoholic extract, the severity of PCOS decreased by 54.86%. Conclusions: These findings lead us to the conclusion that TC extracts and satva as nutritional supplements are useful for treating PCOS and associated symptoms. It is recommended that additional research be conducted to determine the molecular mechanism of action of TC nutritional supplements on PCOS-related changes in metabolic profiles. We also recommend further clinical studies to explore the clinical efficacy and effectiveness of TC nutritional supplements in treating and\/or managing PCOS.<\/jats:p>","DOI":"10.3390\/nu15102238","type":"journal-article","created":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T01:40:02Z","timestamp":1683682802000},"page":"2238","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Regulation of Insulin Resistance, Lipid Profile and Glucose Metabolism Associated with Polycystic Ovary Syndrome by Tinospora cordifolia"],"prefix":"10.3390","volume":"15","author":[{"given":"Ritu","family":"Rani","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, DIT University, Dehradun 248009, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4855-9634","authenticated-orcid":false,"given":"Havagiray R.","family":"Chitme","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, DIT University, Dehradun 248009, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Kukreti","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, DIT University, Dehradun 248009, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2117-294X","authenticated-orcid":false,"given":"Pankaj","family":"Pant","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, DIT University, Dehradun 248009, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5545-6422","authenticated-orcid":false,"given":"Basel A.","family":"Abdel-Wahab","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy, Najran University, Najran P.O. Box 1988, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0591-5210","authenticated-orcid":false,"given":"Masood Medleri","family":"Khateeb","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy, Najran University, Najran P.O. Box 1988, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Shafiuddin","family":"Habeeb","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy, Najran University, Najran P.O. Box 1988, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa B.","family":"Bakir","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Medicine, Najran University, Najran P.O. Box 1988, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100937","DOI":"10.1016\/j.molmet.2020.01.001","article-title":"Metabolic dysfunction in polycystic ovary syndrome: Pathogenic role of androgen excess and potential therapeutic strategies","volume":"35","year":"2020","journal-title":"Mol. Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.2147\/TACG.S200341","article-title":"Genetic Basis of Polycystic Ovary Syndrome (PCOS): Current Perspectives","volume":"12","author":"Khan","year":"2019","journal-title":"Appl. Clin. Genet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1007\/s00404-020-05951-2","article-title":"Association of metabolic and inflammatory markers with polycystic ovarian syndrome (PCOS): An update","volume":"303","year":"2021","journal-title":"Arch. 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