{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:38:28Z","timestamp":1791243508951,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T00:00:00Z","timestamp":1684368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cPioneer\u201d and \u201cleading Goose\u201d R&amp;D Program of Zhejiang","award":["2023C02032"],"award-info":[{"award-number":["2023C02032"]}]},{"name":"\u201cPioneer\u201d and \u201cleading Goose\u201d R&amp;D Program of Zhejiang","award":["2020C02040"],"award-info":[{"award-number":["2020C02040"]}]},{"name":"Key Research and Development Program of Zhejiang Province","award":["2023C02032"],"award-info":[{"award-number":["2023C02032"]}]},{"name":"Key Research and Development Program of Zhejiang Province","award":["2020C02040"],"award-info":[{"award-number":["2020C02040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Torreya grandis meal has a high protein content and an appropriate amino acid ratio, making it an excellent protein source for producing ACE inhibitory peptides. To promote its application in food, medicine, and other fields, an alkaline protease hydrolysate of Torreya grandis was used in this study to isolate and identify a novel angiotensin-converting enzyme inhibitory peptide, VNDYLNW (VW-7), using ultrafiltration, gel chromatography purification, LC-MS\/MS, and in silico prediction. The results show that the IC50 value of VW-7 was 205.98 \u00b5M. The Lineweaver\u2013Burk plot showed that VW-7 had a mixed-type inhibitory effect on ACE. Meanwhile, according to the results of molecular docking, VW-7 demonstrated a strong affinity for ACE (binding energy \u221210 kcal\/mol). VW-7 was bound to ACE through multiple binding sites. In addition, VW-7 could remain active during gastrointestinal digestion in vitro. Nitric oxide (NO) generation in human endothelial cells could rise after receiving a pretreatment with VW-7. These results indicated that Torreya grandis meal protein can be developed into products with antihypertensive function, and VW-7 has broad application prospects in the field of antihypertensive.<\/jats:p>","DOI":"10.3390\/nu15102374","type":"journal-article","created":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:55:29Z","timestamp":1684457729000},"page":"2374","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Purification, Identification, and Inhibitory Mechanisms of a Novel ACE Inhibitory Peptide from Torreya grandis"],"prefix":"10.3390","volume":"15","author":[{"given":"Fenghua","family":"Wu","sequence":"first","affiliation":[{"name":"College of Advanced Agricultural Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8638-4700","authenticated-orcid":false,"given":"Xiaohui","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinzi","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6677-1487","authenticated-orcid":false,"given":"Zhiping","family":"He","sequence":"additional","affiliation":[{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingquan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.foreco.2018.10.028","article-title":"Change in Microbial Communities, Soil Enzyme and Metabolic Activity in a Torreya grandis Plantation in Response to Root Rot Disease","volume":"432","author":"Feng","year":"2019","journal-title":"For. 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