{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:06:51Z","timestamp":1790208411076,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able to form spheroids. We performed the observation and counting of the spheroids with a phase-contrast optical microscope, finding a decrease in the number of spheroids formed in the plates after 24 and 48 h treatment with 250 \u00b5g\/mL of cultivar Romina strawberry extract. We also characterized the spheroids morphology by DNA binding fluorescent-stain observation, hematoxylin and eosin stain, and Masson\u2019s trichrome stain. Finally, the real-time PCR showed a reduced expression of extracellular matrix genes after strawberry treatment. Overall, our data suggest that the fruit extract of this strawberry cultivar may be a useful therapeutic adjuvant for the management of uterine leiomyosarcoma.<\/jats:p>","DOI":"10.3390\/nu15112557","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T02:27:30Z","timestamp":1685500050000},"page":"2557","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The In Vitro Effects of Romina Strawberry Extract on 3D Uterine Leiomyosarcoma Cells"],"prefix":"10.3390","volume":"15","author":[{"given":"Stefania","family":"Greco","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Universit\u00e0 Politecnica delle Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Pellegrino","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Universit\u00e0 Politecnica delle Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8151-9132","authenticated-orcid":false,"given":"Francesca","family":"Giampieri","sequence":"additional","affiliation":[{"name":"Research Group on Food, Nutritional Biochemistry and Health, Universidad Europea del Atl\u00e1ntico, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2593-8535","authenticated-orcid":false,"given":"Franco","family":"Capocasa","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Environmental Sciences, Universit\u00e0 Politecnica delle Marche, 60100 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Delli Carpini","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Universit\u00e0 Politecnica delle Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7250-1782","authenticated-orcid":false,"given":"Maurizio","family":"Battino","sequence":"additional","affiliation":[{"name":"Research Group on Food, Nutritional Biochemistry and Health, Universidad Europea del Atl\u00e1ntico, 39011 Santander, Spain"},{"name":"Department of Clinical Sciences, Universit\u00e0 Politecnica delle Marche, 60126 Ancona, Italy"},{"name":"International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9307-812X","authenticated-orcid":false,"given":"Bruno","family":"Mezzetti","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Environmental Sciences, Universit\u00e0 Politecnica delle Marche, 60100 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9427-1391","authenticated-orcid":false,"given":"Stefano Raffaele","family":"Giannubilo","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Universit\u00e0 Politecnica delle Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ciavattini","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Universit\u00e0 Politecnica delle Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4400-3786","authenticated-orcid":false,"given":"Pasquapina","family":"Ciarmela","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, Universit\u00e0 Politecnica delle Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s00404-022-06531-2","article-title":"Diagnostic and prognostic value of Bcl-2 in uterine leiomyosarcoma","volume":"307","author":"Travaglino","year":"2023","journal-title":"Arch. 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