{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:29:44Z","timestamp":1791854984830,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172180"],"award-info":[{"award-number":["32172180"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["ZR2020KC009"],"award-info":[{"award-number":["ZR2020KC009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["202261040"],"award-info":[{"award-number":["202261040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Project of Natural Science Foundation of Shandong Province in China","award":["32172180"],"award-info":[{"award-number":["32172180"]}]},{"name":"Key Project of Natural Science Foundation of Shandong Province in China","award":["ZR2020KC009"],"award-info":[{"award-number":["ZR2020KC009"]}]},{"name":"Key Project of Natural Science Foundation of Shandong Province in China","award":["202261040"],"award-info":[{"award-number":["202261040"]}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["32172180"],"award-info":[{"award-number":["32172180"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["ZR2020KC009"],"award-info":[{"award-number":["ZR2020KC009"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["202261040"],"award-info":[{"award-number":["202261040"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Bacteriocins production is one of important beneficial characteristics of probiotics, which has antibacterial property against intestinal pathogens and is helpful for regulating intestinal flora. To investigate the impact of bacteriocin-producing probiotics on gut microecology, bacteriocin-producing Lactiplantibacillus plantarum YRL45 was orally administered to mice. The results revealed that it promoted the release of cytokines and improved the phagocytic activity of peritoneal macrophages to activate the immune regulation system. L. plantarum YRL45 was conducive to maintaining the morphology of colon tissue without inflammation and increasing the ratio of villus height to crypt depth in the ileum. The gene expression levels of Muc2, ZO-1 and JAM-1 were significantly up-regulated in the ileum and colon, and the gene expression of Cramp presented an upward trend with L. plantarum YRL45 intervention. Moreover, L. plantarum YRL45 remarkably enhanced the levels of immunoglobulins sIgA, IgA and IgG in the intestine of mice. The 16S rRNA gene analysis suggested that L. plantarum YRL45 administration up-regulated the relative abundance of the beneficial bacteria Muribaculaceae and Akkermansia, down-regulated the abundance of the pathogenic bacteria Lachnoclostridium, and promoted the production of acetic acid, propionic acid and total short-chain fatty acids (SCFAs) in mice feces. Our findings indicated that L. plantarum YRL45 had the potential to be developed as a novel probiotic to regulate the intestinal barrier by altering gut microbiota to enhance intestinal immunity and ameliorate intestinal flora balance.<\/jats:p>","DOI":"10.3390\/nu15153437","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T11:13:06Z","timestamp":1691061186000},"page":"3437","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Bacteriocin-Producing Lactiplantibacillus plantarum YRL45 Enhances Intestinal Immunity and Regulates Gut Microbiota in Mice"],"prefix":"10.3390","volume":"15","author":[{"given":"Yushan","family":"Bu","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China"},{"name":"Food Laboratory of Zhongyuan, Luohe 462300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yisuo","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China"},{"name":"Food Laboratory of Zhongyuan, Luohe 462300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China"},{"name":"Food Laboratory of Zhongyuan, Luohe 462300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinxue","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8968-4263","authenticated-orcid":false,"given":"Huaxi","family":"Yi","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China"},{"name":"Food Laboratory of Zhongyuan, Luohe 462300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1111\/1541-4337.12565","article-title":"Effectiveness of probiotics, prebiotics, and prebiotic-like components in common functional foods","volume":"19","author":"Peng","year":"2020","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dami\u00e1n, M.R., Cortes-Perez, N.G., Quintana, E.T., Ortiz-Moreno, A., Noguez, C.G., Cruce\u00f1o-Casarrubias, C.E., Pardo, M.E.S., and Berm\u00fadez-Humar\u00e1n, L.G. (2022). Functional foods, nutraceuticals and probiotics: A focus on human health. Microorganisms, 10.","DOI":"10.3390\/microorganisms10051065"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ijfoodmicro.2009.05.005","article-title":"In vivo study on the effectiveness of pediocin PA-1 and Pediococcus acidilactici UL5 at inhibiting Listeria monocytogenes","volume":"133","author":"Dabour","year":"2009","journal-title":"Int. J. Food Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.fshw.2021.11.008","article-title":"Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice","volume":"11","author":"Qiao","year":"2022","journal-title":"Food Sci. Hum. Well."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5518825","DOI":"10.1155\/2021\/5518825","article-title":"Bacteriocins: Potential for human health","volume":"2021","author":"Huang","year":"2021","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"594","DOI":"10.3389\/fmicb.2018.00594","article-title":"Application of bacteriocins and protective cultures in dairy food preservation","volume":"9","author":"Silva","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"J\u00f3zefiak, D., Kiero\u0144czyk, B., Ju\u015bkiewicz, J., Zdu\u0144czyk, Z., Rawski, M., D\u0142ugosz, J., Sip, A., and H\u00f8jberg, O. (2013). Dietary nisin modulates the gastrointestinal microbial ecology and enhances growth performance of the broiler chickens. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0085347"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.tim.2019.03.007","article-title":"Mobilization of microbiota commensals and their bacteriocins for therapeutics","volume":"27","author":"Hols","year":"2019","journal-title":"Trends Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Meijerink, M., van Hemert, S., Taverne, N., Wels, M., de Vos, P., Bron, P.A., Savelkoul, H.F., van Bilsen, J., Kleerebezem, M., and Wells, J.M. (2010). Identification of genetic loci in Lactobacillus plantarum that modulate the immune response of dendritic cells using comparative genome hybridization. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010632"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Umu, \u00d6.C., B\u00e4uerl, C., Oostindjer, M., Pope, P.B., Hern\u00e1ndez, P.E., P\u00e9rez-Mart\u00ednez, G., and Diep, D.B. (2016). The potential of class II bacteriocins to modify gut microbiota to improve host health. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0164036"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.anaerobe.2016.05.001","article-title":"The bacteriocin bactofencin A subtly modulates gut microbial populations","volume":"40","author":"Guinane","year":"2016","journal-title":"Anaerobe"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wang, G., Yu, Y., Garcia-Gutierrez, E., Jin, X., He, Y., Wang, L., Tian, P., Liu, Z., Zhao, J., and Zhang, H. (2019). Lactobacillus acidophilus JCM 1132 strain and its mutant with different bacteriocin-producing behaviour have various in situ effects on the gut microbiota of healthy mice. Microorganisms, 8.","DOI":"10.3390\/microorganisms8010049"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1080\/19490976.2018.1534513","article-title":"Lactobacillus plantarum bacteriocin is associated with intestinal and systemic improvements in diet-induced obese mice and maintains epithelial barrier integrity in vitro","volume":"10","author":"Heeney","year":"2019","journal-title":"Gut Microbes"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bu, Y., Liu, Y., Liu, Y., Wang, S., Liu, Q., Hao, H., and Yi, H. (2022). Screening and probiotic potential evaluation of bacteriocin-producing Lactiplantibacillus plantarum in vitro. Foods, 11.","DOI":"10.3390\/foods11111575"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3872","DOI":"10.3382\/ps\/pex205","article-title":"Immune-enhancing activity of phosvitin by stimulating the production of pro-inflammatory mediator","volume":"96","author":"Lee","year":"2017","journal-title":"Poultry Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8570","DOI":"10.7150\/thno.62046","article-title":"Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota","volume":"11","author":"Tong","year":"2021","journal-title":"Theranostics"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jchromb.2018.09.013","article-title":"A fast and accurate way to determine short chain fatty acids in mouse feces based on GC-MS","volume":"1099","author":"Han","year":"2018","journal-title":"J. Chromatogr. B"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"369","DOI":"10.17219\/acem\/58802","article-title":"The thymus: A forgotten, but very important organ","volume":"25","author":"Zdrojewicz","year":"2016","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3979352","DOI":"10.1155\/2019\/3979352","article-title":"Enhancement of macrophage function by the antimicrobial peptide sublancin protects mice from methicillin-resistant Staphylococcus aureus","volume":"2019","author":"Wang","year":"2019","journal-title":"J. Immunol. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4353580","DOI":"10.1155\/2018\/4353580","article-title":"Prevention of cyclophosphamide-induced immunosuppression in mice with the antimicrobial peptide sublancin","volume":"2018","author":"Wang","year":"2018","journal-title":"J. Immunol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bergstrom, K.S., Kissoon-Singh, V., Gibson, D.L., Ma, C., Montero, M., Sham, H.P., Ryz, N., Huang, T., Velcich, A., and Finlay, B.B. (2010). Muc2 protects against lethal infectious colitis by disassociating pathogenic and commensal bacteria from the colonic mucosa. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1000902"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.3390\/ijms9112205","article-title":"Intestinal structure and function of broiler chickens on diets supplemented with a synbiotic containing Enterococcus faecium and oligosaccharides","volume":"9","author":"Awad","year":"2008","journal-title":"Int. J. Mol. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"9642","DOI":"10.1039\/D1FO01286J","article-title":"Toxic effects of copper on the jejunum and colon of pigs: Mechanisms related to gut barrier dysfunction and inflammation influenced by the gut microbiota","volume":"12","author":"Liao","year":"2021","journal-title":"Food Funct."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ijbiomac.2020.12.073","article-title":"Role of immunoglobulin and antibodies in disease management","volume":"169","author":"Megha","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"183","DOI":"10.2174\/1389557519666191125121407","article-title":"Antimicrobial peptides: Vestiges of past or modern therapeutics?","volume":"20","author":"Mitra","year":"2020","journal-title":"Mini-Rev. Med. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"13088","DOI":"10.1074\/jbc.272.20.13088","article-title":"Identification of CRAMP, a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse","volume":"272","author":"Gallo","year":"1997","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1053\/j.gastro.2011.06.079","article-title":"Cathelicidin signaling via the Toll-like receptor protects against colitis in mice","volume":"141","author":"Koon","year":"2011","journal-title":"Gastroenterology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1053\/j.gastro.2021.12.272","article-title":"Intestinal cathelicidin antimicrobial peptide shapes a protective neonatal gut microbiota against pancreatic autoimmunity","volume":"162","author":"Liang","year":"2022","journal-title":"Gastroenterology"},{"key":"ref_29","first-page":"295","article-title":"Managing the F: B ratio in DM; A review of the role of Firmicutes and Bacteroidetes in diabetes mellitus","volume":"4","author":"Ahmed","year":"2019","journal-title":"Adv. Complement. Altern. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"557","DOI":"10.4254\/wjh.v13.i5.557","article-title":"Gut dysbiosis is associated with poorer long-term prognosis in cirrhosis","volume":"13","author":"Maslennikov","year":"2021","journal-title":"World J. Hepatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1089\/omi.2020.0116","article-title":"Microbiome and longevity: High abundance of longevity-linked Muribaculaceae in the gut of the long-living rodent Spalax leucodon","volume":"24","author":"Sibai","year":"2020","journal-title":"OMICS"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"103687","DOI":"10.1016\/j.jff.2019.103687","article-title":"Consumption of mung bean (Vigna radiata L.) attenuates obesity, ameliorates lipid metabolic disorders and modifies the gut microbiota composition in mice fed a high-fat diet","volume":"64","author":"Hou","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1111\/1751-7915.13410","article-title":"Akkermansia muciniphila is a promising probiotic","volume":"12","author":"Zhang","year":"2019","journal-title":"Microb. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"777218","DOI":"10.3389\/fcell.2021.777218","article-title":"Analysis of intestinal microflora and metabolites from mice with DSS-induced IBD treated with Schistosoma soluble egg antigen","volume":"9","author":"Zhu","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10408398.2020.1854675","article-title":"The role of short-chain fatty acids in immunity, inflammation and metabolism","volume":"62","author":"Yao","year":"2020","journal-title":"Crit. Rev. Food Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"109201","DOI":"10.1016\/j.jnutbio.2022.109201","article-title":"Impact of anthocyanin component and metabolite of saskatoon berry on gut microbiome and relationship with fecal short chain fatty acids in diet-induced insulin resistant mice","volume":"111","author":"Zhao","year":"2023","journal-title":"J. Nutr. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1892441","DOI":"10.1080\/19490976.2021.1892441","article-title":"Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development","volume":"13","author":"Kim","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wang, Y., Zou, Y., Wang, J., Ma, H., Zhang, B., and Wang, S. (2020). The protective effects of 2\u2032-fucosyllactose against E. coli O157 infection are mediated by the regulation of gut microbiota and the inhibition of pathogen adhesion. Nutrients, 12.","DOI":"10.3390\/nu12051284"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3964","DOI":"10.1038\/s41467-022-31312-5","article-title":"Imbalanced gut microbiota fuels hepatocellular carcinoma development by shaping the hepatic inflammatory microenvironment","volume":"13","author":"Schneider","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1039\/C4FO00731J","article-title":"Beneficial effects of soy milk and fiber on high cholesterol diet-induced alteration of gut microbiota and inflammatory gene expression in rats","volume":"6","author":"Lee","year":"2015","journal-title":"Food Funct."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/15\/3437\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:25:02Z","timestamp":1760127902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/15\/3437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,3]]},"references-count":40,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["nu15153437"],"URL":"https:\/\/doi.org\/10.3390\/nu15153437","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,3]]}}}