{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T10:55:39Z","timestamp":1791111339177,"version":"4.1.0"},"reference-count":72,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Council, Thailand","award":["N21A650789"],"award-info":[{"award-number":["N21A650789"]}]},{"name":"research Institute of High Human Performance and Health Promotion, Khon Kaen University, Thailand","award":["N21A650789"],"award-info":[{"award-number":["N21A650789"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Due to the rising demand for supplements targeting cognitive enhancement and dry eye together with the health benefits of anthocyanins, we have developed a functional soup containing an anthocyanin-rich functional ingredient, or \u201cAnthaplex,\u201d and assessed the effects on cognitive function and eye dryness together with the possible mechanisms. A total of 69 male and female health volunteers were randomized and divided into placebo, D2, and D4 groups. All subjects consumed 120 mL of placebo or functional soup containing \u201cAnthaplex\u201d either at 2 or 4 g per serving per day within 5 min in the morning for eight weeks. The cognitive function, working memory, dry eye, AChE, MAO, MAO-A, MAO-B, and GABA-T activities, BDNF, HAC, HDAC, and DNMT activities, pH, and amount of lactic acid-producing bacteria, particularly Lactobacillus and Bifidobacterium spp. in feces, were determined before intervention and after eight weeks of consumption. Subjects who consumed the \u201cAnthaplex\u201d soup had improved cognitive function, working memory, eye dryness, histone acetylation, ACh E suppression, and BDNF with increased Bifidobacterium spp. but decreased pH in feces. These data suggest that \u201cAnthaplex\u201d improves cognitive function and eye dryness via the modulations of the histone acetylation process, gut microbiome, and cholinergic function.<\/jats:p>","DOI":"10.3390\/nu15163499","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T12:38:59Z","timestamp":1691498339000},"page":"3499","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["A Randomized, Double-Blind, Placebo-Controlled Study of an Anthocyanin-Rich Functional Ingredient on Cognitive Function and Eye Dryness in Late Adulthood Volunteers: Roles of Epigenetic and Gut Microbiome Modulations"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7383-2348","authenticated-orcid":false,"given":"Jintanaporn","family":"Wattanathorn","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5286-0788","authenticated-orcid":false,"given":"Terdthai","family":"Tong-un","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wipawee","family":"Thukham-mee","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pongsatorn","family":"Paholpak","sequence":"additional","affiliation":[{"name":"Department Psychiatry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poonsri","family":"Rangseekhajee","sequence":"additional","affiliation":[{"name":"Department Psychiatry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"ref_1","first-page":"50","article-title":"Functional food how big is the market?","volume":"12","author":"Hilliam","year":"2000","journal-title":"World Food Ingred."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6390650","DOI":"10.1155\/2019\/6390650","article-title":"Healthy foods as proxy for functional foods: Consumers\u2019 awareness, perception, and demand for natural functional foods in Pakistan","volume":"2019","author":"Ali","year":"2019","journal-title":"Int. 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