{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:25:00Z","timestamp":1760149500315,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T00:00:00Z","timestamp":1692403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Funds for Education Department of Heilongjiang Province of China","award":["2022-KYYWF-0807","2019-KYYWF-1220","QMSI2021M-14"],"award-info":[{"award-number":["2022-KYYWF-0807","2019-KYYWF-1220","QMSI2021M-14"]}]},{"name":"Qiqihar Academy of Medical Sciences Item of China","award":["2022-KYYWF-0807","2019-KYYWF-1220","QMSI2021M-14"],"award-info":[{"award-number":["2022-KYYWF-0807","2019-KYYWF-1220","QMSI2021M-14"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Pu-erh tea is recognized for its weight loss effects, but its potential association with gut microbiota and metabolites remains unclear. This research explored the alterations in gut flora and metabolite composition upon treatment with a co-fermented Pu-erh tea with an aqueous corn silk extract (CPC) in obese mice by employing integrated 16S ribosomal RNA gene sequencing and untargeted metabolomics processes. For 8 weeks, mice were fed control, high-fat, and high-fat diets which included a 46 mg\/mL CPC extract. The CPC extract the alleviated high-fat diet (HFD), it stimulated systemic chronic inflammation, and it reduced the body weight, daily energy consumption, and adipose tissue weight of the mice. It also modified the gut microbiota composition and modulated the Lactobacillus, Bifidobacterium, Allobaculum, Turicibacter, and Rikenella genera. Fecal metabolomics analysis revealed that the CPC extract influenced the caffeine, cysteine, methionine, tryptophan, biotin metabolism pathways, primary bile acid, and steroid biosynthesis. This research revealed that the CPC extract could inhibit HFD-stimulated abnormal weight gain and adipose tissue accumulation in mice, and modulate mice gut microbiota composition and multiple metabolic pathways.<\/jats:p>","DOI":"10.3390\/nu15163642","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T01:46:56Z","timestamp":1692582416000},"page":"3642","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Modulatory Effects of Co-Fermented Pu-erh Tea with Aqueous Corn Silk Extract on Gut Microbes and Fecal Metabolites in Mice Fed High-Fat Diet"],"prefix":"10.3390","volume":"15","author":[{"given":"Lin","family":"Ding","sequence":"first","affiliation":[{"name":"Research Center of Microecological Engineering Technology, Office of Academic Research, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Guan","sequence":"additional","affiliation":[{"name":"Research Center of Microecological Engineering Technology, Office of Academic Research, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Research Center of Microecological Engineering Technology, Office of Academic Research, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Guo","sequence":"additional","affiliation":[{"name":"Research Center of Microecological Engineering Technology, Office of Academic Research, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuangang","family":"Zang","sequence":"additional","affiliation":[{"name":"Research Center of Microecological Engineering Technology, Office of Academic Research, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchao","family":"Liu","sequence":"additional","affiliation":[{"name":"Research Center of Microecological Engineering Technology, Office of Academic Research, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Ren","sequence":"additional","affiliation":[{"name":"Basic Medical Science College, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jicheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Key Laboratory of Natural Medicines for Anticancer, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1161\/CIRCRESAHA.120.316101","article-title":"Obesity phenotypes, diabetes, and cardiovascular diseases","volume":"126","author":"Piche","year":"2020","journal-title":"Circ. Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Malesza, I.J., Malesza, M., Walkowiak, J., Mussin, N., Walkowiak, D., Aringazina, R., Bartkowiak-Wieczorek, J., and Madry, E. (2021). High-fat, western-style diet, systemic inflammation, and gut microbiota: A narrative review. Cells, 10.","DOI":"10.3390\/cells10113164"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s11154-019-09512-0","article-title":"Dietary lipids, gut microbiota and lipid metabolism","volume":"20","author":"Schoeler","year":"2019","journal-title":"Rev. Endocr. Metab. Disord."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s00253-016-8006-6","article-title":"Interaction of gut microbiota with bile acid metabolism and its influence on disease states","volume":"101","author":"Staley","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/j.molmed.2015.09.001","article-title":"Bile acid-activated receptors, intestinal microbiota, and the treatment of metabolic disorders","volume":"21","author":"Fiorucci","year":"2015","journal-title":"Trends Mol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4971","DOI":"10.1038\/s41467-019-12896-x","article-title":"Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism","volume":"10","author":"Huang","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"129783","DOI":"10.1016\/j.foodchem.2021.129783","article-title":"Evolution analysis of flavor-active compounds during artificial fermentation of Pu-erh tea","volume":"357","author":"Deng","year":"2021","journal-title":"Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"737539","DOI":"10.3389\/fnut.2021.737539","article-title":"The impact of citrus-tea cofermentation process on chemical composition and contents of Pu-erh tea: An integrated metabolomics study","volume":"8","author":"Xu","year":"2021","journal-title":"Front. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"935612","DOI":"10.3389\/fnut.2022.935612","article-title":"Modulation of gut microbiota and fecal metabolites by corn silk among high-fat diet-induced hypercholesterolemia mice","volume":"9","author":"Ding","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6978","DOI":"10.1021\/acs.jafc.8b04909","article-title":"Ripened Pu-erh tea extract protects mice from obesity by modulating gut microbiota composition","volume":"67","author":"Lu","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4250","DOI":"10.1002\/jsfa.11777","article-title":"Theabrownin isolated from Pu-erh tea regulates Bacteroidetes to improve metabolic syndrome of rats induced by high-fat, high-sugar and high-salt diet","volume":"102","author":"Yue","year":"2022","journal-title":"J. Sci. Food Agric."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/S1499-3872(14)60024-2","article-title":"Inflammatory response of macrophages in infection","volume":"13","author":"Zhang","year":"2014","journal-title":"Hepatobiliary Pancreat. Dis. Int."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lecube, A., and Lopez-Cano, C. (2019). Obesity, a diet-induced inflammatory disease. Nutrients, 11.","DOI":"10.3390\/nu11102284"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.tem.2011.01.002","article-title":"Effects of the gut microbiota on obesity and glucose homeostasis","volume":"22","author":"Greiner","year":"2011","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"G496","DOI":"10.1152\/ajpgi.00291.2013","article-title":"Dissociation of hepatic insulin resistance from susceptibility of nonalcoholic fatty liver disease induced by a high-fat and high-carbohydrate diet in mice","volume":"306","author":"Asai","year":"2014","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/nature18846","article-title":"Diet-microbiota interactions as moderators of human metabolism","volume":"535","author":"Sonnenburg","year":"2016","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/s40168-018-0510-8","article-title":"The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism","volume":"6","author":"Just","year":"2018","journal-title":"Microbiome"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/s41575-020-0269-9","article-title":"Gut microbiota and human NAFLD: Disentangling microbial signatures from metabolic disorders","volume":"17","author":"Vigliotti","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Grigor\u2019eva, I.N. (2020). Gallstone disease, obesity and the Firmicutes\/Bacteroidetes ratio as a possible biomarker of gut dysbiosis. J. Pers. Med., 11.","DOI":"10.3390\/jpm11010013"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Magne, F., Gotteland, M., Gauthier, L., Zazueta, A., Pesoa, S., Navarrete, P., and Balamurugan, R. (2020). The Firmicutes\/Bacteroidetes ratio: A relevant marker of gut dysbiosis in obese patients?. Nutrients, 12.","DOI":"10.3390\/nu12051474"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.copbio.2017.08.004","article-title":"Intestinal Lactobacillus in health and disease, a driver or just along for the ride?","volume":"49","author":"Heeney","year":"2018","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5439","DOI":"10.1111\/1750-3841.15971","article-title":"Lactobacillus alleviated obesity induced by high-fat diet in mice","volume":"86","author":"Song","year":"2021","journal-title":"J. Food Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.anaerobe.2004.06.004","article-title":"Allobaculum stercoricanis gen. nov., sp. nov., isolated from canine feces","volume":"10","author":"Greetham","year":"2004","journal-title":"Anaerobe"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"690138","DOI":"10.3389\/fnut.2021.690138","article-title":"Allobaculum involves in the modulation of intestinal ANGPTLT4 expression in mice treated by high-fat diet","volume":"8","author":"Zheng","year":"2021","journal-title":"Front. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6229","DOI":"10.1007\/s11033-020-05611-3","article-title":"Molecular link between dietary fibre, gut microbiota and health","volume":"47","author":"Kumar","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"108594","DOI":"10.1016\/j.jnutbio.2021.108594","article-title":"Metagenomic insights into the effects of Urtica dioica vegetable on the gut microbiota of C57BL\/6J obese mice, particularly the composition of Clostridia","volume":"91","author":"Fan","year":"2021","journal-title":"J. Nutr. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kemis, J.H., Linke, V., Barrett, K.L., Boehm, F.J., Traeger, L.L., Keller, M.P., Rabaglia, M.E., Schueler, K.L., Stapleton, D.S., and Gatti, D.M. (2019). Genetic determinants of gut microbiota composition and bile acid profiles in mice. PLoS Genet., 15.","DOI":"10.1101\/571075"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Johnson, A.M., Costanzo, A., Gareau, M.G., Armando, A.M., Quehenberger, O., Jameson, J.M., and Olefsky, J.M. (2015). High fat diet causes depletion of intestinal eosinophils associated with intestinal permeability. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0122195"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5532","DOI":"10.1038\/s41598-021-84928-w","article-title":"Gut microbiota markers associated with obesity and overweight in Italian adults","volume":"11","author":"Palmas","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4275","DOI":"10.1039\/D0FO00133C","article-title":"Tomato seed oil attenuates hyperlipidemia and modulates gut microbiota in C57BL\/6J mice","volume":"11","author":"He","year":"2020","journal-title":"Food Funct."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1016\/j.bbagen.2017.05.019","article-title":"Ferroptosis: Role of lipid peroxidation, iron and ferritinophagy","volume":"1861","year":"2017","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1271\/bbb.130083","article-title":"Dietary L-cysteine improves the antioxidative potential and lipid metabolism in rats fed a normal diet","volume":"77","author":"Lee","year":"2013","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5821","DOI":"10.1039\/D1FO00269D","article-title":"L-Threonine upregulates the expression of beta-defensins by activating the NF-kappaB signaling pathway and suppressing SIRT1 expression in porcine intestinal epithelial cells","volume":"12","author":"Wang","year":"2021","journal-title":"Food Funct."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.jcmgh.2019.11.011","article-title":"Biotin supplementation ameliorates murine colitis by preventing NF-kappaB activation","volume":"9","author":"Skupsky","year":"2020","journal-title":"Cell Mol. Gastroenterol. Hepatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1146\/annurev-nutr-082018-124344","article-title":"Bile acids as metabolic regulators and nutrient sensors","volume":"39","author":"Chiang","year":"2019","journal-title":"Annu. Rev. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3727\/105221618X15156018385515","article-title":"Bile acid metabolism in liver pathobiology","volume":"18","author":"Chiang","year":"2018","journal-title":"Gene Expr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0278-6915(95)00093-3","article-title":"Caffeine consumption","volume":"34","author":"Barone","year":"1996","journal-title":"Food Chem. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1099\/mic.0.043612-0","article-title":"Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source","volume":"157","author":"Summers","year":"2011","journal-title":"Microbiol. (Read.)"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/biof.5520340405","article-title":"Inhibitory effects of caffeine and its metabolites on intracellular lipid accumulation in murine 3T3-L1 adipocytes","volume":"34","author":"Nakabayashi","year":"2008","journal-title":"Biofactors"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Ahmed, W.H., Boulet, N., Briot, A., Ryan, B.J., Kinsella, G.K., O\u2019Sullivan, J., Les, F., Mercader-Barcelo, J., Henehan, G.T.M., and Carpene, C. (2021). Novel facet of an old dietary molecule? direct influence of caffeine on glucose and biogenic amine handling by human adipocytes. Molecules, 26.","DOI":"10.3390\/molecules26133831"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/16\/3642\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:37:25Z","timestamp":1760128645000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/16\/3642"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,19]]},"references-count":40,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["nu15163642"],"URL":"https:\/\/doi.org\/10.3390\/nu15163642","relation":{},"ISSN":["2072-6643"],"issn-type":[{"type":"electronic","value":"2072-6643"}],"subject":[],"published":{"date-parts":[[2023,8,19]]}}}