{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:23:41Z","timestamp":1787628221096,"version":"build-2736575974"},"reference-count":152,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T00:00:00Z","timestamp":1692748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The South Dakota Beef Industry Council"},{"name":"The South Dakota State University Research"},{"name":"Scholarship"},{"name":"Creative Activity Challenge Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>This feeding trial evaluated the impact of the Dietary Approaches to Stop Hypertension diet on changes in plasma choline, choline metabolites, and ceramides in obese older adults; 28 adults consumed 3oz (n = 15) or 6oz (n = 13) of beef within a standardized DASH diet for 12 weeks. Plasma choline, betaine, methionine, dimethylglycine (DMG), phosphatidylcholine (PC), lysophosphotidylcholine (LPC), sphingomyelin, trimethylamine-N-oxide (TMAO), L-carnitine, ceramide, and triglycerides were measured in fasted blood samples. Plasma LPC, sphingomyelin, and ceramide species were also quantified. In response to the study diet, with beef intake groups combined, plasma choline decreased by 9.6% (p = 0.012); DMG decreased by 10% (p = 0.042); PC decreased by 51% (p &lt; 0.001); total LPC increased by 281% (p &lt; 0.001); TMAO increased by 26.5% (p &lt; 0.001); total ceramide decreased by 22.1% (p &lt; 0.001); and triglycerides decreased by 18% (p = 0.021). All 20 LPC species measured increased (p &lt; 0.01) with LPC 16:0 having the greatest response. Sphingomyelin 16:0, 18:0, and 18:1 increased (all p &lt; 0.001) by 10.4%, 22.5%, and 24%, respectively. In contrast, we observed that sphingomyelin 24:0 significantly decreased by 10%. Ceramide 22:0 and 24:0 decreased by 27.6% and 10.9% (p &lt; 0.001), respectively, and ceramide 24:1 increased by 36.8% (p = 0.013). Changes in choline and choline metabolites were in association with anthropometric and cardiometabolic outcomes. These findings show the impact of the DASH diet on choline metabolism in older adults and demonstrate the influence of diet to modify circulating LPC, sphingomyelin, and ceramide species.<\/jats:p>","DOI":"10.3390\/nu15173687","type":"journal-article","created":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T08:01:21Z","timestamp":1692777681000},"page":"3687","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Changes in Choline Metabolites and Ceramides in Response to a DASH-Style Diet in Older Adults"],"prefix":"10.3390","volume":"15","author":[{"given":"Brianna N.","family":"Tate","sequence":"first","affiliation":[{"name":"Department of Animal Science, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4540-1903","authenticated-orcid":false,"given":"Gary P.","family":"Van Guilder","sequence":"additional","affiliation":[{"name":"High Altitude Exercise Physiology Department, Western Colorado University, Gunnison, CO 81231, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa","family":"Aly","sequence":"additional","affiliation":[{"name":"Department of Applied Health Science, Indiana University School of Public Health, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Spence","sequence":"additional","affiliation":[{"name":"Department of Applied Health Science, Indiana University School of Public Health, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Elena","family":"Diaz-Rubio","sequence":"additional","affiliation":[{"name":"Proteomic and Metabolomics Facility, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2942-2357","authenticated-orcid":false,"given":"Henry H.","family":"Le","sequence":"additional","affiliation":[{"name":"Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth L.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph W.","family":"McFadden","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8874-1978","authenticated-orcid":false,"given":"Cydne A.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Applied Health Science, Indiana University School of Public Health, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.nut.2009.07.004","article-title":"Body composition changes with aging: The cause or the result of alterations in metabolic rate and macronutrient oxidation?","volume":"26","author":"Gallagher","year":"2010","journal-title":"Nutrition"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.jdiacomp.2019.03.007","article-title":"Body composition changes in diabetes and aging","volume":"33","author":"Ganji","year":"2019","journal-title":"J. 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