{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:42:53Z","timestamp":1790192573010,"version":"4.1.0"},"reference-count":51,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T00:00:00Z","timestamp":1693699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004233","name":"First Project Grant","doi-asserted-by":"publisher","award":["PAID-06-22"],"award-info":[{"award-number":["PAID-06-22"]}],"id":[{"id":"10.13039\/501100004233","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004233","name":"First Project Grant","doi-asserted-by":"publisher","award":["PAIDPD-22"],"award-info":[{"award-number":["PAIDPD-22"]}],"id":[{"id":"10.13039\/501100004233","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Postdoctoral Research Fostering Grant","award":["PAID-06-22"],"award-info":[{"award-number":["PAID-06-22"]}]},{"name":"Postdoctoral Research Fostering Grant","award":["PAIDPD-22"],"award-info":[{"award-number":["PAIDPD-22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Cystic Fibrosis-related gut dysbiosis (CFRGD) has become a recognised complication in children with this condition, and current evidence remains insufficient to guide the selection of probiotic strains for supplementation treatments. The aim of this study was to characterise the effect of three probiotic strains on CFRGD by means of a dynamic in vitro simulation of the colonic fermentation (SHIME\u00ae). The configuration of the system included three bioreactors colonised with the faecal inoculum of a child with cystic fibrosis. For 20 days, each bioreactor was supplied daily with either Lacticaseibacillus rhamnosus GG (ATCC 53103 TM), Limosilactobacillus reuteri (DSM 17938) or Lactiplantibacillus plantarum (DSM 22266). The baseline microbiota was characterised by a high abundance of Prevotella, Faecalibacterium and Acidaminococcus genera. After 20 days of supplementation, L. rhamnosus and L. plantarum reduced Prevotella significantly, and the three strains led to increased Faecalibacterium and Bifidobacterium and decreased Acidaminococcus, with some of these changes being maintained 10 days after ceasing supplementation. The metabolic activity remained unaltered in terms of short-chain fatty acids, but branched-chain fatty acids showed a significant decrease, especially with L. plantarum. Additionally, ammonia decreased at 20 days of supplementation, and lactate continuously increased with the three strains. The effects on colonic microbiota of L. rhamnosus, L. reuteri or L. plantarum were established, including increased beneficial bacteria, such as Faecalibacterium, and beneficial metabolites such as lactate; and on the other hand, a reduction in pathogenic genera, including Prevotella or Acidaminococcus and branched-chain fatty acids, overall supported their use as probiotics in the context of CFRGD.<\/jats:p>","DOI":"10.3390\/nu15173846","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T02:43:20Z","timestamp":1693795400000},"page":"3846","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effect of Lactobacillaceae Probiotics on Colonic Microbiota and Metabolite Production in Cystic Fibrosis: A Comparative In Vitro Study"],"prefix":"10.3390","volume":"15","author":[{"given":"Andrea","family":"Asensio-Grau","sequence":"first","affiliation":[{"name":"Institute of Food Engineering (IIA-FoodUPV), Polytechnic University of Valencia, Camino de Vera s\/n, 46022 Val\u00e8ncia, Spain"},{"name":"Joint Research Unit NutriCura<sup>PDig<\/sup>, Avda. Fernando Abril Martorell 106, 46026 Val\u00e8ncia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquim","family":"Calvo-Lerma","sequence":"additional","affiliation":[{"name":"Institute of Food Engineering (IIA-FoodUPV), Polytechnic University of Valencia, Camino de Vera s\/n, 46022 Val\u00e8ncia, Spain"},{"name":"Joint Research Unit NutriCura<sup>PDig<\/sup>, Avda. Fernando Abril Martorell 106, 46026 Val\u00e8ncia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Ferriz-Jord\u00e1n","sequence":"additional","affiliation":[{"name":"Institute of Food Engineering (IIA-FoodUPV), Polytechnic University of Valencia, Camino de Vera s\/n, 46022 Val\u00e8ncia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1258-6128","authenticated-orcid":false,"given":"Jorge","family":"Garc\u00eda-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Advanced Food Microbiology Centre (CAMA), Polytechnic University of Valencia, Camino de Vera s\/n, 46022 Val\u00e8ncia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Heredia","sequence":"additional","affiliation":[{"name":"Institute of Food Engineering (IIA-FoodUPV), Polytechnic University of Valencia, Camino de Vera s\/n, 46022 Val\u00e8ncia, Spain"},{"name":"Joint Research Unit NutriCura<sup>PDig<\/sup>, Avda. Fernando Abril Martorell 106, 46026 Val\u00e8ncia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6132-3167","authenticated-orcid":false,"given":"Ana","family":"Andr\u00e9s","sequence":"additional","affiliation":[{"name":"Institute of Food Engineering (IIA-FoodUPV), Polytechnic University of Valencia, Camino de Vera s\/n, 46022 Val\u00e8ncia, Spain"},{"name":"Joint Research Unit NutriCura<sup>PDig<\/sup>, Avda. Fernando Abril Martorell 106, 46026 Val\u00e8ncia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.pcl.2016.04.005","article-title":"Nutrition and Growth in Cystic Fibrosis. Pediatr","volume":"63","author":"Lusman","year":"2016","journal-title":"Clin. 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