{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:01:11Z","timestamp":1787029271187,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Obesity and heavy metals, such as lead (Pb), are detrimental to the adult brain because they impair cognitive function and structural plasticity. However, the effects of co-administration of Pb and a high-fat diet (HFD) on the developing cerebellum is not clearly elucidated. We investigated the effects of Pb exposure (0.3% lead acetate) on developing cerebellum in the pups of an HFD-fed obese rat model. One week before mating, we fed a chow diet (CD) or HFD to the rats for one week and additionally administered Pb to HFD-fed female SD rats. Thereafter, treatment with Pb and a HFD was continued during the gestational and lactational periods. On postnatal day 21, the pups were euthanized to sample the brain tissue and blood for further analysis. Blood Pb levels were significantly higher in HFD-fed rats than in CD-fed rats. Histologically, the prominent degeneration of Purkinje cells was induced by the co-administration of Pb and HFD. The calbindin-28Kd-, GAD67-, NMDAR1-, and PSD95-immunopositive Purkinje cells and inhibitory synapse-forming pinceau structures were significantly decreased following Pb and HFD co-administration. MBP-immunoreactive myelinated axonal fibers were also impaired by HFD but were significantly damaged by the co-administration of HFD and Pb. Oxidative stress-related Nrf2\u2013HO1 signaling was activated by HFD feeding, and Pb exposure further aggravated oxidative stress, as demonstrated by the consumption of endogenous anti-oxidant in HFD\u2013Pb rats. The pro-inflammatory response was also increased by the co-administration of HFD and Pb in the cerebellum of the rat offspring. The present results suggest that HFD and Pb treatment during the gestational and lactational periods are harmful to cerebellar development.<\/jats:p>","DOI":"10.3390\/nu15204325","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T02:11:01Z","timestamp":1696990261000},"page":"4325","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Gestational and Lactational Lead Exposure and High Fat Diet Feeding on Cerebellar Development of Postnatal Rat Offspring"],"prefix":"10.3390","volume":"15","author":[{"given":"Jin Seok","family":"Seo","sequence":"first","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin Hyo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine and Jesaeng-Euise Clinical Anatomy Center, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6084-6698","authenticated-orcid":false,"given":"Hyung-Sun","family":"Won","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine and Jesaeng-Euise Clinical Anatomy Center, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4748-6007","authenticated-orcid":false,"given":"Miyoung","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine and Jesaeng-Euise Clinical Anatomy Center, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Seop","family":"Nahm","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Min","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine and Jesaeng-Euise Clinical Anatomy Center, Wonkwang University, Iksan 54538, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1177\/01926233211046933","article-title":"Comparative Milestones in Rodent and Human Postnatal Central Nervous System Development","volume":"49","author":"Zeiss","year":"2021","journal-title":"Toxicol. Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1161\/ATVBAHA.107.151092","article-title":"Metabolic syndrome pandemic","volume":"28","author":"Grundy","year":"2008","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Glastras, S.J., Chen, H., Pollock, C.A., and Saad, S. (2018). Maternal obesity increases the risk of metabolic disease and impacts renal health in offspring. Biosci. Rep., 38.","DOI":"10.1042\/BSR20180050"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S2213-8587(16)30107-3","article-title":"Influence of maternal obesity on the long-term health of offspring","volume":"5","author":"Godfrey","year":"2017","journal-title":"Lancet Diabetes Endocrinol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.2337\/dc18-2581","article-title":"Children exposed to maternal obesity or gestational diabetes mellitus during early fetal development have hypothalamic alterations that predict future weight gain","volume":"42","author":"Page","year":"2019","journal-title":"Diabetes Care"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1289\/ehp.11241","article-title":"Lead exposures in US children, 2008: Implications for prevention","volume":"116","author":"Levin","year":"2008","journal-title":"Environ. Health Perspect."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1021\/acs.chemrestox.8b00193","article-title":"Biochemical and molecular bases of lead-induced toxicity in mammalian systems and possible mitigations","volume":"31","author":"Singh","year":"2018","journal-title":"Chem. Res. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.envpol.2017.07.015","article-title":"Elevated lead levels from e-waste exposure are linked to decreased olfactory memory in children","volume":"231","author":"Zhang","year":"2017","journal-title":"Environ. Pollut."},{"key":"ref_9","unstructured":"ATSDR (2023, March 25). Substance Priority List. Agency for Toxic Substances and Disease Registry, Available online: https:\/\/www.atsdr.cdc.gov\/spl\/index.html."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1289\/ehp.1307420","article-title":"Environmental lead exposure and attention deficit\/hyperactivity disorder symptom domains in a community sample of South Korean school-age children","volume":"123","author":"Hong","year":"2015","journal-title":"Environ. Health Perspect."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"12631","DOI":"10.1007\/s11356-019-04799-7","article-title":"Lead (Pb) in the tissues of Anatidae, Ardeidae, Sternidae and Laridae of the Northern Hemisphere: A review of environmental studies","volume":"26","author":"Korbecki","year":"2019","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_12","unstructured":"Rees, N., and Fuller, R. (2020). The Toxic Truth: Children\u2019s Exposure to Lead Pollution Undermines a Generation of Future Potential, UNICEF and Pure Earth. Available online: https:\/\/www.unicef.org\/reports\/toxic-truth-childrens-exposure-to-lead-pollution-2020."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.coesh.2018.10.002","article-title":"Preventing another lead (Pb) in drinking water crisis: Lessons from the Washington DC and Flint MI contamination events","volume":"7","author":"Roy","year":"2019","journal-title":"Curr. Opin. Environ. Sci. Health"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chibowska, K., Korbecki, J., Gutowska, I., Metryka, E., Tarnowski, M., Goschorska, M., Barczak, K., Chlubek, D., and Baranowska-Bosiacka, I. (2020). Pre- and neonatal exposure to lead (Pb) induces neuroinflammation in the forebrain cortex, hippocampus and cerebellum of rat pups. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21031083"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Nam, S.M., Choi, S.H., Cho, H.J., Seo, J.S., Choi, M., Nahm, S.S., Chang, B.J., and Nah, S.Y. (2020). Ginseng gintonin attenuates lead-induced rat cerebellar impairments during gestation and lactation. Biomolecules, 10.","DOI":"10.3390\/biom10030385"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Cecil, K.M., Brubaker, C.J., Adler, C.M., Dietrich, K.N., Altaye, M., Egelhoff, J.C., Wessel, S., Elangovan, I., Hornung, R., and Jarvis, K. (2008). Decreased brain volume in adults with childhood lead exposure. PLoS Med., 5.","DOI":"10.1371\/journal.pmed.0050112"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1177\/0009922819839237","article-title":"Children with autism spectrum disorder and lead poisoning: Diagnostic challenges and management complexities","volume":"58","author":"Hauptman","year":"2019","journal-title":"Clin. Pediatr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/brain\/awg014","article-title":"Lead neurotoxicity in children: Basic mechanisms and clinical correlates","volume":"126","author":"Lidsky","year":"2003","journal-title":"Brain"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1007\/s12011-018-1572-y","article-title":"Ascorbic acid supplementation prevents the detrimental effects of prenatal and postnatal lead exposure on the Purkinje cell and related proteins in the cerebellum of developing rats","volume":"190","author":"Nam","year":"2019","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1007\/s00204-015-1515-8","article-title":"Revisiting mobilisation of skeletal lead during pregnancy based on monthly sampling and cord\/maternal blood lead relationships confirm placental transfer of lead","volume":"90","author":"Gulson","year":"2015","journal-title":"Arch. Toxicol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Nam, S.M., Seo, M., Seo, J.S., Rhim, H., Nahm, S.S., Cho, I.H., Chang, B.J., Kim, H.J., Choi, S.H., and Nah, S.Y. (2019). Ascorbic acid mitigates D-galactose-induced brain aging by increasing hippocampal neurogenesis and improving memory function. Nutrients, 11.","DOI":"10.3390\/nu11010176"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"10138","DOI":"10.1523\/JNEUROSCI.3159-04.2004","article-title":"Postsynaptic density protein-95 regulates NMDA channel gating and surface expression","volume":"24","author":"Lin","year":"2004","journal-title":"J. Neurosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1113\/jphysiol.2002.034918","article-title":"PSD-95 regulates NMDA receptors in developing cerebellar granule neurons of the rat","volume":"548","author":"Losi","year":"2003","journal-title":"J. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1038\/nn1903","article-title":"BDNF induces transport of PSD95 to dendrites through PI3K-AKT signaling after NMDA receptor activation","volume":"10","author":"Yoshii","year":"2007","journal-title":"Nat. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s11011-014-9560-1","article-title":"GABA receptors in brain development, function, and injury","volume":"30","author":"Wu","year":"2015","journal-title":"Metab. Brain Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s12974-020-01782-9","article-title":"Microglial physiological properties and interactions with synapses are altered at presymptomatic stages in a mouse model of Huntington\u2019s disease pathology","volume":"17","author":"Savage","year":"2020","journal-title":"J. Neuroinflamm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7499","DOI":"10.1523\/JNEUROSCI.0392-12.2012","article-title":"Panglial gap junctional communication is essential for maintenance of myelin in the CNS","volume":"32","author":"Tress","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.5114\/aoms.2015.56342","article-title":"Brain-derived neurotrophic factor and its clinical implications","volume":"11","author":"Bathina","year":"2015","journal-title":"Arch. Med. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1007\/s12011-018-1354-6","article-title":"Ascorbic acid attenuates lead-induced alterations in the synapses in the developing rat cerebellum","volume":"187","author":"Nam","year":"2019","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"964236","DOI":"10.1155\/2014\/964236","article-title":"Overweight and obesity epidemic in developing countries: A problem with diet, physical activity, or socioeconomic status?","volume":"2014","author":"Bhurosy","year":"2014","journal-title":"Sci. World J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.brainresrev.2005.02.004","article-title":"Lead neurotoxicity: From exposure to molecular effects","volume":"49","author":"Toscano","year":"2005","journal-title":"Brain Res. Brain Res. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.toxlet.2017.08.015","article-title":"Both physiology and epidemiology support zero tolerable blood lead levels","volume":"280","author":"Shefa","year":"2017","journal-title":"Toxicol. Lett."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Leff, T., Stemmer, P., Tyrrell, J., and Jog, R. (2018). Diabetes and exposure to environmental lead (Pb). Toxics, 6.","DOI":"10.3390\/toxics6030054"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1475-2840-12-9","article-title":"Blood lead is significantly associated with metabolic syndrome in Korean adults: An analysis based on the Korea National Health and Nutrition Examination Survey (KNHANES), 2008","volume":"12","author":"Rhee","year":"2013","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/cne.24065","article-title":"Impaired constitutive and regenerative neurogenesis in adult hyperglycemic zebrafish","volume":"525","author":"Dorsemans","year":"2017","journal-title":"J. Comp. Neurol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1042\/CS20190180","article-title":"Obesity-induced type 2 diabetes impairs neurological recovery after stroke in correlation with decreased neurogenesis and persistent atrophy of parvalbumin-positive interneurons","volume":"133","author":"Pintana","year":"2019","journal-title":"Clin. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.brainres.2008.08.041","article-title":"Receptor for advanced glycation end products and neuronal deficit in the fatal brain edema of diabetic ketoacidosis","volume":"1238","author":"Hoffman","year":"2008","journal-title":"Brain Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10860","DOI":"10.1523\/JNEUROSCI.2744-07.2007","article-title":"Sequential postsynaptic maturation governs the temporal order of GABAergic and glutamatergic synaptogenesis in rat embryonic cultures","volume":"27","author":"Deng","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1002\/(SICI)1096-9861(19980309)392:2<164::AID-CNE2>3.0.CO;2-0","article-title":"Single Purkinje cell can innervate multiple classes of projection neurons in the cerebellar nuclei of the rat: A light microscopic and ultrastructural triple-tracer study in the rat","volume":"392","author":"Teune","year":"1998","journal-title":"J. Comp. Neurol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1177\/0748233714545624","article-title":"Histological study on hippocampus, amygdala and cerebellum following low lead exposure during prenatal and postnatal brain development in rats","volume":"32","author":"Barkur","year":"2016","journal-title":"Toxicol. Ind. Health"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Neuwirth, L.S., Phillips, G.R., and El Idrissi, A. (2018). Perinatal Pb2+ exposure alters the expression of genes related to the neurodevelopmental GABA-shift in postnatal rats. J. Biomed. Sci., 25.","DOI":"10.1186\/s12929-018-0450-4"},{"key":"ref_42","first-page":"315418","article-title":"Developing central nervous system and vulnerability to platinum compounds","volume":"2011","author":"Bernocchi","year":"2011","journal-title":"Chemother. Res. Pract."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Sandoval-Salazar, C., Ram\u00edrez-Emiliano, J., Trejo-Bahena, A., Oviedo-Sol\u00eds, C.I., and Sol\u00eds-Ortiz, M.S. (2016). A high-fat diet decreases GABA concentration in the frontal cortex and hippocampus of rats. Biol. Res., 49.","DOI":"10.1186\/s40659-016-0075-6"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.brainres.2009.09.063","article-title":"Insulin and IGF-I prevent brain atrophy and DNA loss in diabetes","volume":"1303","author":"Serbedzija","year":"2009","journal-title":"Brain Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Nam, S.M., Seo, J.S., Nahm, S.S., and Chang, B.J. (2019). Effects of ascorbic acid on osteopontin expression and axonal myelination in the developing cerebellum of lead-exposed rat pups. Int. J. Environ. Res. Public. Health, 16.","DOI":"10.3390\/ijerph16060983"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"19096","DOI":"10.3390\/ijms160819096","article-title":"Lead poisoning disturbs oligodendrocytes differentiation involved in decreased expression of NCX3 inducing intracellular calcium overload","volume":"16","author":"Ma","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"478","DOI":"10.3892\/etm.2013.1435","article-title":"Effect of oxidative stress on heme oxygenase-1 expression in patients with gestational diabetes mellitus","volume":"7","author":"Xin","year":"2014","journal-title":"Exp. Ther. Med."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Liu, C.M., Tian, Z.K., Zhang, Y.J., Ming, Q.L., Ma, J.Q., and Ji, L.P. (2020). Effects of gastrodin against lead-induced brain injury in mice associated with the Wnt\/Nrf2 pathway. Nutrients, 12.","DOI":"10.3390\/nu12061805"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.nbd.2009.04.002","article-title":"Effects of high fat diet on Morris maze performance, oxidative stress, and inflammation in rats: Contributions of maternal diet","volume":"35","author":"White","year":"2009","journal-title":"Neurobiol. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3389\/fncel.2018.00114","article-title":"Effect of chronic oxidative stress on neuroinflammatory response mediated by CD4+T cells in neurodegenerative diseases","volume":"12","year":"2018","journal-title":"Front. Cell Neurosci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s10571-017-0510-4","article-title":"BDNF: A Key Factor with Multipotent Impact on Brain Signaling and Synaptic Plasticity","volume":"38","author":"Lietzau","year":"2018","journal-title":"Cell. Mol. Neurobiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.neuint.2008.11.005","article-title":"Maternal supply of BDNF to mouse fetal brain through the placenta","volume":"54","author":"Kodomari","year":"2009","journal-title":"Neurochem. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1002\/cbin.11500","article-title":"Potential role of NGF, BDNF, and their receptors in oligodendrocytes differentiation from neural stem cell: An in vitro study","volume":"45","author":"Langhnoja","year":"2021","journal-title":"Cell Biol. Int."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.neuint.2010.05.015","article-title":"Maternal obesity impairs hippocampal BDNF production and spatial learning performance in young mouse offspring","volume":"57","author":"Tozuka","year":"2010","journal-title":"Neurochem. Int."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1161\/STROKEAHA.114.006692","article-title":"Brain-derived neurotrophic factor administration mediated oligodendrocyte differentiation and myelin formation in subcortical ischemic stroke","volume":"46","author":"Fuentes","year":"2015","journal-title":"Stroke"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5695","DOI":"10.2174\/1381612811319320005","article-title":"Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases","volume":"19","author":"Prioletta","year":"2013","journal-title":"Curr. Pharm. Des."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Fuente-Mart\u00edn, E., Mellado-Gil, J.M., Cobo-Vuilleumier, N., Mart\u00edn-Montalvo, A., Romero-Zerbo, S.Y., Diaz Contreras, I., Hmadcha, A., Soria, B., Martin Bermudo, F., and Reyes, J.C. (2019). Dissecting the brain\/islet axis in metabesity. Genes, 10.","DOI":"10.3390\/genes10050350"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1743-7075-3-16","article-title":"Control of blood glucose in type 2 diabetes without weight loss by modification of diet composition","volume":"3","author":"Gannon","year":"2006","journal-title":"Nutr. Metab."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/jdi.13619","article-title":"Effects of intermittent very-low calorie diet on glycemic control and cardiovascular risk factors in obese patients with type 2 diabetes mellitus: A randomized controlled trial","volume":"13","author":"Umphonsathien","year":"2022","journal-title":"J. Diabetes Investig."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/20\/4325\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:04:31Z","timestamp":1760130271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/20\/4325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,10]]},"references-count":59,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["nu15204325"],"URL":"https:\/\/doi.org\/10.3390\/nu15204325","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,10]]}}}