{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T10:28:11Z","timestamp":1783247291733,"version":"3.54.6"},"reference-count":53,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang National Key Research and Development program of China","award":["2021C04010"],"award-info":[{"award-number":["2021C04010"]}]},{"name":"Zhejiang National Key Research and Development program of China","award":["2022SNJF008"],"award-info":[{"award-number":["2022SNJF008"]}]},{"name":"Nine-part Cooperation Project of Agriculture and Rural Development of Zhejiang Province","award":["2021C04010"],"award-info":[{"award-number":["2021C04010"]}]},{"name":"Nine-part Cooperation Project of Agriculture and Rural Development of Zhejiang Province","award":["2022SNJF008"],"award-info":[{"award-number":["2022SNJF008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>To investigate the role of gastrointestinal (GI) polysaccharide fermentation in alleviating constipation, two polysaccharide fractions were isolated from a soluble fiber extract with determined anti-constipation activity: a 2.04 kDa neutral fraction (SSP-1) contained 99.29% glucose, and a 41.66 kDa acidic fraction (SSP-2) contained 63.85% uronic acid. After mice were given loperamide for 14 d to induce constipation, the GI transit rate increased significantly in the SSP-1 group (p &lt; 0.05) but not in the SSP-2 group. The stool weight in the SSP-2 group was significantly higher than that in SSP-1 (383.60 mg vs. 226.23 mg) (p &lt; 0.05). Both SSP-1 and SSP-2 groups had significantly increased serum gastrin and motilin levels (p &lt; 0.05) and changes in their fecal short-chain fatty acid (SCFA) profiles, while SSP-1 showed better fermentation properties than SSP-2 in terms of statistically higher fecal contents of acetic acid and total SCFAs (p &lt; 0.05). Bioinformatic analysis indicated that SSP-1 upregulated bacteria such as Oscillibacter to improve SCFA metabolism and stimulate GI hormone secretion, while SSP-2 had less influence on the gut microbiota. These results suggest that the neutral polysaccharide with superior GI fermentation properties exerted beneficial effects on constipation, while the less fermentable pectic fraction might act as a stool-bulking agent.<\/jats:p>","DOI":"10.3390\/nu15204364","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T14:59:59Z","timestamp":1697295599000},"page":"4364","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Gastrointestinal Fermentable Polysaccharide Is Beneficial in Alleviating Loperamide-Induced Constipation in Mice"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3704-0359","authenticated-orcid":false,"given":"Buyu","family":"Liu","sequence":"first","affiliation":[{"name":"Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3637-8107","authenticated-orcid":false,"given":"Zhiguo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingquan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Hu","sequence":"additional","affiliation":[{"name":"Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5696-3354","authenticated-orcid":false,"given":"Weicheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1053\/j.gastro.2016.02.031","article-title":"Bowel Disorders","volume":"150","author":"Lacy","year":"2016","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cgh.2008.07.005","article-title":"A Gap in Our Understanding: Chronic Constipation and Its Comorbid Conditions","volume":"7","author":"Talley","year":"2009","journal-title":"Clin. 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