{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T07:50:03Z","timestamp":1784620203811,"version":"3.55.0"},"reference-count":54,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T00:00:00Z","timestamp":1698192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"2023\u20132024 Annual Scientific Research Project of Traditional Chinese Medicine Scientific Research Project of Hubei Province","award":["ZY2023Q035"],"award-info":[{"award-number":["ZY2023Q035"]}]},{"name":"2023\u20132024 Annual Scientific Research Project of Traditional Chinese Medicine Scientific Research Project of Hubei Province","award":["2022BGE254"],"award-info":[{"award-number":["2022BGE254"]}]},{"name":"Central Government Guide Local Science and Technology Development Project of Hubei Province","award":["ZY2023Q035"],"award-info":[{"award-number":["ZY2023Q035"]}]},{"name":"Central Government Guide Local Science and Technology Development Project of Hubei Province","award":["2022BGE254"],"award-info":[{"award-number":["2022BGE254"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Carboxymethyl pachymaran (CMP) was treated via high-temperature and cellulase hydrolysis to obtain HTCMP, HTEC-24, and HTEC-48. The chemical structure and in vivo antitumor activities of the four types of CMPs were investigated. Compared with CMP (787.9 kDa), the molecular weights of HTCMP, HTEC-24, and HTEC-48 were decreased to 429.8, 129.9, and 68.6 kDa, respectively. The viscosities and particle sizes of the CMPs could also decrease with the decline in the molecular weights. All the CMPs showed antitumor abilities, but HTEC-24 exhibited the best activity. In the animal study, when curing the spleen and thymus, CMPs displayed immunomodulatory effects by increasing the secretion of IFN-\u03b3 and IL2 in mice. The CMPs also exerted an antitumor ability by regulating the gut microbiota in tumor-bearing mice. Our results established a foundation to develop an antitumor drug with CMP.<\/jats:p>","DOI":"10.3390\/nu15214527","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T09:54:08Z","timestamp":1698227648000},"page":"4527","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Antitumor Activity of Carboxymethyl Pachymaran with Different Molecular Weights Based on Immunomodulatory and Gut Microbiota"],"prefix":"10.3390","volume":"15","author":[{"given":"Yalin","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijia","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7673-9624","authenticated-orcid":false,"given":"Wen","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9380-3424","authenticated-orcid":false,"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ijbiomac.2020.02.334","article-title":"A low-molecular-weight ascophyllan prepared from Ascophyllum nodosum: Optimization, analysis and biological activities","volume":"153","author":"Yu","year":"2020","journal-title":"Int. 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