{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T05:33:28Z","timestamp":1784180008245,"version":"3.55.0"},"reference-count":27,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T00:00:00Z","timestamp":1701216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["2020R1I1A3068253"],"award-info":[{"award-number":["2020R1I1A3068253"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2020R1I1A1A01055554"],"award-info":[{"award-number":["2020R1I1A1A01055554"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2021R1I1A3059702"],"award-info":[{"award-number":["2021R1I1A3059702"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2021R1I1A3047973"],"award-info":[{"award-number":["2021R1I1A3047973"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2021R1I1A1A01040336"],"award-info":[{"award-number":["2021R1I1A1A01040336"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["HR22C1832"],"award-info":[{"award-number":["HR22C1832"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["HI22C1529"],"award-info":[{"award-number":["HI22C1529"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["2020R1I1A3068253"],"award-info":[{"award-number":["2020R1I1A3068253"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["2020R1I1A1A01055554"],"award-info":[{"award-number":["2020R1I1A1A01055554"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["2021R1I1A3059702"],"award-info":[{"award-number":["2021R1I1A3059702"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["2021R1I1A3047973"],"award-info":[{"award-number":["2021R1I1A3047973"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["2021R1I1A1A01040336"],"award-info":[{"award-number":["2021R1I1A1A01040336"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["HR22C1832"],"award-info":[{"award-number":["HR22C1832"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["HI22C1529"],"award-info":[{"award-number":["HI22C1529"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Abnormal lipid metabolism increases the relative risk of kidney disease in patients with a single kidney. Using transcriptome analysis, we investigated whether a high-fat diet leads to abnormalities in lipid metabolism and induces kidney cell-specific damage in unilateral nephrectomy mice. Mice with unilateral nephrectomy fed a high-fat diet for 12 weeks exhibited progressive renal dysfunction in proximal tubules, including lipid accumulation, vacuolization, and cell damage. Ring finger protein 20 (RNF20) is a ligase of nuclear receptor corepressor of peroxisome proliferator-activated receptors (PPARs). The transcriptome analysis revealed the involvement of RNF20-related transcriptome changes in PPAR signaling, lipid metabolism, and water transmembrane transporter under a high-fat diet and unilateral nephrectomy. In vitro treatment of proximal tubular cells with palmitic acid induced lipotoxicity by altering RNF20, PPAR\u03b1, and ATP-binding cassette subfamily A member 1 (ABCA1) expression. PPAR\u03b3 and aquaporin 2 (AQP2) expression decreased in collecting duct cells, regulating genetic changes in the water reabsorption process. In conclusion, a high-fat diet induces lipid accumulation under unilateral nephrectomy via altering RNF20-mediated regulation and causing functional damage to cells as a result of abnormal lipid metabolism, thereby leading to structural and functional kidney deterioration.<\/jats:p>","DOI":"10.3390\/nu15234959","type":"journal-article","created":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T12:01:00Z","timestamp":1701259260000},"page":"4959","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of Ring Finger Protein 20 and Its Downstream Regulation on Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a High-Fat Diet"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9813-3348","authenticated-orcid":false,"given":"You-Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Cell and Matrix Research Institute, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se-Hyun","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Cell and Matrix Research Institute, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5517-9886","authenticated-orcid":false,"given":"Jeong-Hoon","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Cell and Matrix Research Institute, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Division of Nephrology, Department of Intermanl Medicine, Kyungpook National University Chilgok Hospital, Daegu 41404, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7031-5214","authenticated-orcid":false,"given":"Jang-Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Cell and Matrix Research Institute, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee-Yeon","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4648-0324","authenticated-orcid":false,"given":"Chan-Duck","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0953-3343","authenticated-orcid":false,"given":"Sun-Hee","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1561-6508","authenticated-orcid":false,"given":"Tae-Hwan","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-3455","authenticated-orcid":false,"given":"Yong-Lim","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Cell and Matrix Research Institute, Kyungpook National University, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,29]]},"reference":[{"key":"ref_1","unstructured":"del Moral, A.M., and Aguilera Garc\u00eda, C.M. 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