{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:46:06Z","timestamp":1780490766147,"version":"3.54.1"},"reference-count":50,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Infertility, affecting 15 to 25% of couples in the most developed countries, is recognized by the World Health Organization as a public health issue at a global level. Different causes are acknowledged to reduce fertility in both sexes. In particular, about 40\u201350% of cases recognize a male factor. Dietary habits and lifestyle are acknowledged to influence sperm quality and are therefore important modifiable factors in male reproductive health. Conditions such as overweight\/obesity, impaired glucose metabolism and determinants of metabolic syndrome, together with unhealthy lifestyle behavior, i.e., smoking cigarettes and physical inactivity, are suggested to have a negative impact on male fertility. While individual elements and characteristics of the Western diet and habits are considered risk factors for male infertility, the Mediterranean diet (MD) seems to promote reproductive potential for improving sperm quality. It is also interesting to note that previous observational studies reported a positive correlation between the consumption of the single food classes of the MD pattern (i.e., vegetables and fruits, poultry, fish and seafood, whole grains, low-fat dairy products) and the quality of several sperm parameters. To evaluate the relationship between sperm parameters and MD adherence, we performed a cross-sectional study on the seminal data of 300 males (mean age 34.6 \u00b1 9.1 years) who spontaneously referred to our center of reproductive medicine. The evaluation of adherence to MD was performed with a validated 14-point Mediterranean Diet Adherence Screener (MEDAS) questionnaire. Our findings showed that sperm parameters such as sperm count, motility, viability and normal morphology are significantly and positively correlated with MEDAS, independently of BMI and age. In addition, the application of an ROC curve on MEDAS value vs. seminal alterations identified 6.25 as the score threshold value below which altered sperm parameters were more likely to occur [AUC = 0.096 (CI: 0.059\u20130.133; p &lt; 0.00)]. Therefore, adhering to the MD with at least a MEDAS score of 6.26 increases the probability of normozoospermia. Moreover, subjects who had a MEDAS value lower than 6.25 had an Odds Ratio of 6.28 (CI = 3.967\u20139.945) for having at least one altered sperm parameter compared to those who were more adherent to the MD. In conclusion, our findings show that a higher adherence to the MD is associated with better semen parameters, in particular in relation to sperm count, sperm concentration, typical sperm morphology, and sperm progressive motility.<\/jats:p>","DOI":"10.3390\/nu15234989","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T08:36:59Z","timestamp":1701419819000},"page":"4989","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Observational Cross-Sectional Study on Mediterranean Diet and Sperm Parameters"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5333-9249","authenticated-orcid":false,"given":"Gabriel Cosmin","family":"Petre","sequence":"first","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Francini-Pesenti","sequence":"additional","affiliation":[{"name":"Clinical Nutrition Unit, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Di Nisio","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"De Toni","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3264-0937","authenticated-orcid":false,"given":"Giuseppe","family":"Grande","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asia","family":"Mingardi","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Cusmano","sequence":"additional","affiliation":[{"name":"Clinical Nutrition Unit, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Spinella","sequence":"additional","affiliation":[{"name":"Clinical Nutrition Unit, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5817-8141","authenticated-orcid":false,"given":"Alberto","family":"Ferlin","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4736-9051","authenticated-orcid":false,"given":"Andrea","family":"Garolla","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1093\/humupd\/dmv016","article-title":"Infertility around the Globe: New Thinking on Gender, Reproductive Technologies and Global Movements in the 21st Century","volume":"21","author":"Inhorn","year":"2015","journal-title":"Hum. Reprod. Update"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.fertnstert.2019.01.032","article-title":"Home Testing for Male Factor Infertility: A Review of Current Options","volume":"111","author":"Kobori","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1111\/andr.12659","article-title":"Risk Behaviours and Alcohol in Adolescence Are Negatively Associated with Testicular Volume: Results from the Amico-Andrologo Survey","volume":"7","author":"Gianfrilli","year":"2019","journal-title":"Andrology"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"N\u00e4tt, D., Kugelberg, U., Casas, E., Nedstrand, E., Zalavary, S., Henriksson, P., Nijm, C., J\u00e4derquist, J., Sandborg, J., and Flinke, E. (2019). Human Sperm Displays Rapid Responses to Diet. PLoS Biol., 17.","DOI":"10.1371\/journal.pbio.3000559"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.fertnstert.2018.05.025","article-title":"Diet and Men\u2019s Fertility: Does Diet Affect Sperm Quality?","volume":"110","author":"Nassan","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3328","DOI":"10.1093\/humrep\/des311","article-title":"Intake of Food Groups and Idiopathic Asthenozoospermia: A Case-Control Study","volume":"27","author":"Eslamian","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1093\/humrep\/det464","article-title":"Trans Fatty Acid Intake Is Inversely Related to Total Sperm Count in Young Healthy Men","volume":"29","author":"Chavarro","year":"2014","journal-title":"Hum. Reprod."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1016\/j.fertnstert.2013.08.032","article-title":"Semen Quality in Relation to Antioxidant Intake in a Healthy Male Population","volume":"100","author":"Zareba","year":"2013","journal-title":"Fertil. Steril."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.1093\/humrep\/des247","article-title":"Dietary Intake of Antioxidant Nutrients Is Associated with Semen Quality in Young University Students","volume":"27","author":"Mendiola","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.repbio.2019.07.005","article-title":"Diet and Sperm Quality: Nutrients, Foods and Dietary Patterns","volume":"19","author":"James","year":"2019","journal-title":"Reprod. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ferramosca, A., and Zara, V. (2022). Diet and Male Fertility: The Impact of Nutrients and Antioxidants on Sperm Energetic Metabolism. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23052542"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Leisegang, K., Bouic, P.J., Menkveld, R., and Henkel, R.R. (2014). Obesity Is Associated with Increased Seminal Insulin and Leptin alongside Reduced Fertility Parameters in a Controlled Male Cohort. Reprod. Biol. Endocrinol., 12.","DOI":"10.1186\/1477-7827-12-34"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Dominguez, L.J., Di Bella, G., Veronese, N., and Barbagallo, M. (2021). Impact of Mediterranean Diet on Chronic Non-Communicable Diseases and Longevity. Nutrients, 13.","DOI":"10.3390\/nu13062028"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sofi, F., Cesari, F., Abbate, R., Gensini, G.F., and Casini, A. (2008). Adherence to Mediterranean Diet and Health Status: Meta-Analysis. BMJ, 337.","DOI":"10.1136\/bmj.a1344"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1590\/s1677-5538.ibju.2021.0604","article-title":"The Combined Effect of Lifestyle Intervention and Antioxidant Therapy on Sperm DNA Fragmentation and Seminal Oxidative Stress in IVF Patients: A Pilot Study","volume":"48","author":"Humaidan","year":"2022","journal-title":"Int. Braz. J. Urol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.fct.2014.03.017","article-title":"The Effects of Bioactive Compounds from Plant Foods on Mitochondrial Function: A Focus on Apoptotic Mechanisms","volume":"68","author":"Giampieri","year":"2014","journal-title":"Food Chem. Toxicol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Alesi, S., Villani, A., Mantzioris, E., Takele, W.W., Cowan, S., Moran, L.J., and Mousa, A. (2022). Anti-Inflammatory Diets in Fertility: An Evidence Review. Nutrients, 14.","DOI":"10.3390\/nu14193914"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"280","DOI":"10.23736\/S0391-1977.20.03362-3","article-title":"Effects of Mediterranean Diet on Semen Parameters in Healthy Young Adults: A Randomized Controlled Trial","volume":"45","author":"Caruso","year":"2021","journal-title":"Minerva Endocrinol."},{"key":"ref_19","first-page":"215","article-title":"Association between Adherence to the Mediterranean Diet and Semen Quality Parameters in Male Partners of Couples Attempting Fertility","volume":"32","author":"Karayiannis","year":"2016","journal-title":"Hum. Reprod."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/andr.12587","article-title":"Mediterranean Diet and the Risk of Poor Semen Quality: Cross-sectional Analysis of Men Referring to an Italian Fertility Clinic","volume":"7","author":"Ricci","year":"2019","journal-title":"Andrology"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Salas-Huetos, A., Babio, N., Carrell, D.T., Bull\u00f3, M., and Salas-Salvad\u00f3, J. (2019). Adherence to the Mediterranean Diet Is Positively Associated with Sperm Motility: A Cross-Sectional Analysis. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-39826-7"},{"key":"ref_22","first-page":"2945","article-title":"Mediterranean and Western Dietary Patterns Are Related to Markers of Testicular Function among Healthy Men","volume":"30","author":"Mendiola","year":"2015","journal-title":"Hum. Reprod."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Conesa, M.-T., Philippou, E., Pafilas, C., Massaro, M., Quarta, S., Andrade, V., Jorge, R., Chervenkov, M., Ivanova, T., and Dimitrova, D. (2020). Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region. Nutrients, 12.","DOI":"10.3390\/nu12102960"},{"key":"ref_24","unstructured":"World Health Organization (2021). WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization. [6th ed.]."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Belladelli, F., Basran, S., and Eisenberg, M.L. (2023). Male Fertility and Physical Exercise. World J. Mens Health, 41.","DOI":"10.5534\/wjmh.220199"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1111\/andr.13504","article-title":"Improving Standard Practices in Studies Using Results from Basic Human Semen Examination","volume":"11","author":"Esteves","year":"2023","journal-title":"Andrology"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"\u0141akoma, K., Kukharuk, O., and \u015ali\u017c, D. (2023). The Influence of Metabolic Factors and Diet on Fertility. Nutrients, 15.","DOI":"10.3390\/nu15051180"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1007\/s40618-022-01741-6","article-title":"Management of Male Factor Infertility: Position Statement from the Italian Society of Andrology and Sexual Medicine (SIAMS): Endorsing Organization: Italian Society of Embryology, Reproduction, and Research (SIERR)","volume":"45","author":"Ferlin","year":"2022","journal-title":"J. Endocrinol. Investig."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Leisegang, K., Sengupta, P., Agarwal, A., and Henkel, R. (2021). Obesity and Male Infertility: Mechanisms and Management. Andrologia, 53.","DOI":"10.1111\/and.13617"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1159\/000502119","article-title":"Prognostic Value of Ultrasound Stratigraphy in Long-Term Weight Loss: Results from a Nutritional Counseling Program","volume":"12","author":"Petre","year":"2019","journal-title":"Obes. Facts"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1111\/andr.12861","article-title":"The Impact of Metabolically Healthy Obesity in Primary Infertile Men: Results from a Cross-sectional Study","volume":"8","author":"Cazzaniga","year":"2020","journal-title":"Andrology"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Agius, R., Pace, N.P., and Fava, S. (2023). Phenotyping Obesity: A Focus on Metabolically Healthy Obesity and Metabolically Unhealthy Normal Weight. Diabetes Metab. Res. Rev.","DOI":"10.1002\/dmrr.3725"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.orcp.2023.04.001","article-title":"Adherence to the DASH, MeDi, and MIND Diet Scores and the Incidence of Metabolically Unhealthy Phenotypes","volume":"17","author":"Golzarand","year":"2023","journal-title":"Obes. Res. Clin. Pract."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1093\/ajcn\/nqy181","article-title":"Effect of Nut Consumption on Semen Quality and Functionality in Healthy Men Consuming a Western-Style Diet: A Randomized Controlled Trial","volume":"108","author":"Moraleda","year":"2018","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/aja.2012.46","article-title":"The Roles of Omega-3 and Omega-6 Fatty Acids in Idiopathic Male Infertility","volume":"14","author":"Safarinejad","year":"2012","journal-title":"Asian J. Androl."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Funes, A.K., Sim\u00f3n, L., Colombo, R., Avena, M.V., Moncl\u00fas, M., Crescitelli, J., Cabrillana, M.E., Conte, M.I., Cayado, N., and Boarelli, P. (2021). Impact of High Fat Diet on the Sterol Regulatory Element-Binding Protein 2 Cholesterol Pathway in the Testicle. Mol. Hum. Reprod., 27.","DOI":"10.1093\/molehr\/gaab023"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Abdollahzadeh, S., Riasi, A., Tavalaee, M., Jafarpour, F., and Nasr-Esfahani, M.H. (2023). Omega 6\/Omega 3 Ratio Is High in Individuals with Increased Sperm DNA Fragmentation. Reprod. Sci.","DOI":"10.1007\/s43032-023-01313-w"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/j.1439-0272.2006.00735.x","article-title":"Decreased Polyunsaturated and Increased Saturated Fatty Acid Concentration in Spermatozoa from Asthenozoospermic Males as Compared with Normozoospermic Males","volume":"38","author":"Tavilani","year":"2006","journal-title":"Andrologia"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/andr.12348","article-title":"Dietary Fatty Acids Influence Sperm Quality and Function","volume":"5","author":"Ferramosca","year":"2017","journal-title":"Andrology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2899","DOI":"10.1093\/humrep\/des298","article-title":"Dietary Patterns and Semen Quality in Young Men","volume":"27","author":"Gaskins","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Danielewicz, A., Morze, J., Przyby\u0142owicz, M., and Przyby\u0142owicz, K.E. (2019). Association of the Dietary Approaches to Stop Hypertension, Physical Activity, and Their Combination with Semen Quality: A Cross-Sectional Study. Nutrients, 12.","DOI":"10.3390\/nu12010039"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/andr.12430","article-title":"Dietary Patterns and Semen Quality: A Systematic Review and Meta-analysis of Observational Studies","volume":"6","author":"Arab","year":"2018","journal-title":"Andrology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1093\/humupd\/dmx006","article-title":"Dietary Patterns, Foods and Nutrients in Male Fertility Parameters and Fecundability: A Systematic Review of Observational Studies","volume":"23","year":"2017","journal-title":"Hum. Reprod. Update"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Garolla, A., Petre, G.C., Francini-Pesenti, F., De Toni, L., Vitagliano, A., Di Nisio, A., Grande, G., and Foresta, C. (2022). Systematic Review and Critical Analysis on Dietary Supplements for Male Infertility: From a Blend of Ingredients to a Rationale Strategy. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.824078"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1210\/en.2019-00487","article-title":"Obesity, Neuroinflammation, and Reproductive Function","volume":"160","author":"Lainez","year":"2019","journal-title":"Endocrinology"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Chianese, R., and Pierantoni, R. (2021). Mitochondrial Reactive Oxygen Species (ROS) Production Alters Sperm Quality. Antioxidants, 10.","DOI":"10.3390\/antiox10010092"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/13685538.2022.2044468","article-title":"Diet and Prostate Health: An Underrated Tool?","volume":"25","author":"Basile","year":"2022","journal-title":"Aging Male"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jri.2013.05.001","article-title":"Markers of Semen Inflammation: Supplementary Semen Analysis?","volume":"100","author":"Condorelli","year":"2013","journal-title":"J. Reprod. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/S0090-4295(99)00096-5","article-title":"Diet and Benign Prostatic Hyperplasia: A Study in Greece","volume":"54","author":"Lagiou","year":"1999","journal-title":"Urology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1093\/aje\/kwm389","article-title":"Dietary Patterns, Supplement Use, and the Risk of Symptomatic Benign Prostatic Hyperplasia: Results from the Prostate Cancer Prevention Trial","volume":"167","author":"Kristal","year":"2008","journal-title":"Am. J. Epidemiol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/23\/4989\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:36:09Z","timestamp":1760132169000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/15\/23\/4989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,1]]},"references-count":50,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["nu15234989"],"URL":"https:\/\/doi.org\/10.3390\/nu15234989","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,1]]}}}