{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:55:23Z","timestamp":1760144123391,"version":"build-2065373602"},"reference-count":59,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T00:00:00Z","timestamp":1710892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008783","name":"National Research Council of Science and Technology","doi-asserted-by":"publisher","award":["CRC22021-100","HI20C0363"],"award-info":[{"award-number":["CRC22021-100","HI20C0363"]}],"id":[{"id":"10.13039\/501100008783","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korea Health Technology R&amp;D Project through the Korea Health Industry Development Institute","award":["CRC22021-100","HI20C0363"],"award-info":[{"award-number":["CRC22021-100","HI20C0363"]}]},{"name":"Ministry of Health and Welfare, Republic of Korea","award":["CRC22021-100","HI20C0363"],"award-info":[{"award-number":["CRC22021-100","HI20C0363"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>We aimed to identify the mechanism underlying the preventive effects of non-alcoholic fatty liver disease (NAFLD) through Platycodi Radix consumption using liver proteomic and bioinformatic analysis. C57BL\/6J mice were categorized into three groups: those receiving a standard chow diet (NCD), those on a high-fat diet (HFD), and those on an HFD supplemented with 5% Platycodi Radix extract (PRE). After a 12-week period, PRE-fed mice exhibited a noteworthy prevention of hepatic steatosis. Protein identification and quantification in liver samples were conducted using LC-MS\/MS. The identified proteins were analyzed through Ingenuity Pathway Analysis software, revealing a decrease in proteins associated with FXR\/RXR activation and a concurrent increase in cholesterol biosynthesis proteins in the PRE-treated mouse liver. Subsequent network analysis predicted enhanced bile acid synthesis from these proteins. Indeed, the quantity of bile acids, which was reduced in HFD conditions, increased in the PRE group, accompanied by an elevation in the expression of synthesis-related proteins. Our findings suggest that the beneficial effects of PRE in preventing hepatic steatosis may be mediated, at least in part, through the modulation of FXR\/RXR activation, cholesterol biosynthesis, and bile acid synthesis pathways.<\/jats:p>","DOI":"10.3390\/nu16060893","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T05:56:07Z","timestamp":1710914167000},"page":"893","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Platycodi Radix Extract Prevents Hepatic Steatosis by Enhancing Bile Acid Synthesis in a High-Fat Diet-Induced Fatty Liver Mouse Model"],"prefix":"10.3390","volume":"16","author":[{"given":"Wooyoung","family":"Kim","sequence":"first","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"},{"name":"Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea"},{"name":"Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woon Hee","family":"Baek","sequence":"additional","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"},{"name":"Department of Bio-Analytic Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Ho","family":"Yun","sequence":"additional","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"},{"name":"Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi Jeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Yeop","family":"Lee","sequence":"additional","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"},{"name":"Department of Bio-Analytic Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0360-4435","authenticated-orcid":false,"given":"Gun-Hwa","family":"Kim","sequence":"additional","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"},{"name":"Department of Bio-Analytic Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Il","family":"Kim","sequence":"additional","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"},{"name":"Department of Bio-Analytic Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8020-8914","authenticated-orcid":false,"given":"Hye Gwang","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6699-4886","authenticated-orcid":false,"given":"Edmond Changkyun","family":"Park","sequence":"additional","affiliation":[{"name":"Digital Omics Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea"},{"name":"Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea"},{"name":"Department of Bio-Analytic Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.eururo.2007.04.069","article-title":"Obesity and prostate cancer: Epidemiology and clinical implications","volume":"52","author":"Buschemeyer","year":"2007","journal-title":"Eur. 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