{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T17:01:38Z","timestamp":1791910898477,"version":"4.3.4"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kolmar BNH."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>There is growing evidence linking gut microbiota to overall health, including obesity risk and associated diseases. Lactiplantibacillus plantarum SKO-001, a probiotic strain isolated from Angelica gigas, has been reported to reduce obesity by controlling the gut microbiome. In this double-blind, randomised clinical trial, we aimed to evaluate the efficacy and safety of SKO-001 in reducing body fat. We included 100 participants randomised into SKO-001 or placebo groups (1:1) for 12 weeks. Dual-energy X-ray absorptiometry was used to objectively evaluate body fat reduction. Body fat percentage (p = 0.016), body fat mass (p = 0.02), low-density lipoprotein-cholesterol levels (p = 0.025), and adiponectin levels (p = 0.023) were lower in the SKO-001 group than in the placebo group after 12 weeks of SKO-001 consumption. In the SKO-001 group, the subcutaneous fat area (p = 0.003), total cholesterol levels (p = 0.003), and leptin levels (p = 0.014) significantly decreased after 12 weeks of SKO-001 consumption compared with baseline values. Additionally, SKO-001 did not cause any severe adverse reactions. In conclusion, SKO-001 is safe and effective for reducing body fat and has the potential for further clinical testing in humans.<\/jats:p>","DOI":"10.3390\/nu16081137","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T03:34:37Z","timestamp":1712892877000},"page":"1137","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["A 12-Week, Single-Centre, Randomised, Double-Blind, Placebo-Controlled, Parallel-Design Clinical Trial for the Evaluation of the Efficacy and Safety of Lactiplantibacillus plantarum SKO-001 in Reducing Body Fat"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1660-2973","authenticated-orcid":false,"given":"Seon Mi","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, College of Korean Medicine, Semyung University, Semyeong-ro 65, Jecheon-si 27136, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8962-0334","authenticated-orcid":false,"given":"Jeong-Su","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, College of Korean Medicine, Semyung University, Semyeong-ro 65, Jecheon-si 27136, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4488-0527","authenticated-orcid":false,"given":"Sang Back","family":"Kim","sequence":"additional","affiliation":[{"name":"Food Science R&D Center, Kolmar BNH Co., Ltd., 61, Heolleung-ro 8-gil, Seocho-gu, Seoul 06800, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"Food Science R&D Center, Kolmar BNH Co., Ltd., 61, Heolleung-ro 8-gil, Seocho-gu, Seoul 06800, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee","family":"Seo","sequence":"additional","affiliation":[{"name":"Food Science R&D Center, Kolmar BNH Co., Ltd., 61, Heolleung-ro 8-gil, Seocho-gu, Seoul 06800, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9594-7173","authenticated-orcid":false,"given":"Hak Sung","family":"Lee","sequence":"additional","affiliation":[{"name":"Food Science R&D Center, Kolmar BNH Co., Ltd., 61, Heolleung-ro 8-gil, Seocho-gu, Seoul 06800, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dai, H., Alsalhe, T.A., Chalghaf, N., Ricc\u00f2, M., Bragazzi, N.L., and Wu, J. (2020). The global burden of disease attributable to high body mass index in 195 countries and territories, 1990-2017: An analysis of the Global Burden of Disease Study. PLoS Med., 17.","DOI":"10.1371\/journal.pmed.1003198"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13424","DOI":"10.1038\/s41598-019-49462-w","article-title":"Discrepant gut microbiota markers for the classification of obesity-related metabolic abnormalities","volume":"9","author":"Zeng","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Perna, S., Ilyas, Z., Giacosa, A., Gasparri, C., Peroni, G., Faliva, M.A., Rigon, C., Naso, M., Riva, A., and Petrangolini, G. (2021). Is probiotic supplementation useful for the management of body weight and other anthropometric measures in adults affected by overweight and obesity with metabolic related diseases? A systematic review and meta-analysis. Nutrients, 13.","DOI":"10.3390\/nu13020666"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ilhan, Z.E., DiBaise, J.K., Dautel, S.E., Isern, N.G., Kim, Y.M., Hoyt, D.W., Schepmoes, A.A., Brewer, H.M., Weitz, K.K., and Metz, T.O. (2020). Temporospatial shifts in the human gut microbiome and metabolome after gastric bypass surgery. NPJ Biofilms Microbiomes, 6.","DOI":"10.1038\/s41522-020-0122-5"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1007\/s11695-024-07080-0","article-title":"Prospective monitoring of small intestinal bacterial overgrowth after gastric bypass: Clinical, biological, and gas chromatographic aspects","volume":"34","author":"Florent","year":"2024","journal-title":"Obes. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zhou, Y.D., Liang, F.X., Tian, H.R., Luo, D., Wang, Y.Y., and Yang, S.R. (2023). Mechanisms of gut microbiota-immune-host interaction on glucose regulation in type 2 diabetes. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1121695"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wici\u0144ski, M., G\u0119balski, J., Go\u0142\u0119biewski, J., and Malinowski, B. (2020). Probiotics for the treatment of overweight and obesity in humans\u2014A review of clinical trials. Microorganisms, 8.","DOI":"10.3390\/microorganisms8081148"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Puebla-Barragan, S., and Reid, G. (2021). Probiotics in cosmetic and personal care products: Trends and challenges. Molecules, 26.","DOI":"10.3390\/molecules26051249"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1097\/MCO.0b013e328348c06d","article-title":"Gut microbiota interactions with obesity, insulin resistance and type 2 diabetes","volume":"14","author":"Esteve","year":"2011","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s13073-022-01092-0","article-title":"Metabolic control by the microbiome","volume":"14","author":"Cox","year":"2022","journal-title":"Genome Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3920\/BM2012.0069","article-title":"The development of probiotic treatment in obesity: A review","volume":"5","author":"Mekkes","year":"2014","journal-title":"Benef. Microbes"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"947367","DOI":"10.3389\/fnut.2022.947367","article-title":"Lactobacillus plantarum alleviates obesity by altering the composition of the gut microbiota in high-fat diet-fed mice","volume":"9","author":"Ma","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1007\/s00394-023-03096-x","article-title":"Anti-obesity effects of Lactiplantibacillus plantarum SKO-001 in high-fat diet-induced obese mice","volume":"62","author":"Choi","year":"2023","journal-title":"Eur. J. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"790046","DOI":"10.3389\/fendo.2021.790046","article-title":"Efficacy and Safety of Lactobacillus plantarum K50 on Lipids in Koreans With Obesity: A Randomized, Double-Blind Controlled Clinical Trial","volume":"12","author":"Sohn","year":"2022","journal-title":"Front. Endocrinol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mo, S.J., Lee, K., Hong, H.J., Hong, D.K., Jung, S.H., Park, S.D., Shim, J.J., and Lee, J.L. (2022). Effects of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 on Overweight and the Gut Microbiota in Humans: Randomized, Double-Blinded, Placebo-Controlled Clinical Trial. Nutrients, 14.","DOI":"10.3390\/nu14122484"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"92","DOI":"10.4093\/dmj.2021.0370","article-title":"Effect of Lactobacillus plantarum LMT1-48 on Body Fat in Overweight Subjects: A Randomized, Double-Blind, Placebo-Controlled Trial","volume":"47","author":"Sohn","year":"2023","journal-title":"Diabetes Metab. J."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Cho, Y.-G., Jung, J., Kang, J.-H., Kwon, J.S., Yu, S.P., and Baik, T.G. (2017). Effect of a herbal extract powder (YY-312) from Imperata cylindrica Beauvois, Citrus unshiu Markovich, and Evodia officinalis Dode on body fat mass in overweight adults: A 12-week, randomized, double-blind, placebo-controlled, parallel-group clinical trial. BMC Complement. Altern. Med., 17.","DOI":"10.1186\/s12906-017-1871-4"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7570\/jomes23016","article-title":"Evaluation and treatment of obesity and its comorbidities: 2022 update of clinical practice guidelines for obesity by the Korean Society for the Study of Obesity","volume":"32","author":"Kim","year":"2023","journal-title":"J. Obes. Metab. Syndr."},{"key":"ref_19","unstructured":"(2024, January 01). Ministry of Health and Welfare, South Korea: 2020 Community Integrated Health Promotion Project. Available online: https:\/\/www.mohw.go.kr\/board.es?mid=a10107010100&bid=0046&act=view&list_no=372345&tag=&cg_code=&list_depth=1."},{"key":"ref_20","first-page":"1","article-title":"Impact of Lactiplantibacillus plantarum inducia on metabolic and antioxidative response in cholesterol and BMI variable indices: Randomised, double-blind, placebo-controlled trials","volume":"14","author":"Gerulis","year":"2023","journal-title":"Benef. Microbes"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"110490","DOI":"10.1016\/j.foodres.2021.110490","article-title":"Protective and ameliorating effects of probiotics against diet-induced obesity: A review","volume":"147","author":"Tang","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1006\/bbrc.1999.0255","article-title":"Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity","volume":"257","author":"Arita","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/372425a0","article-title":"Positional cloning of the mouse obese gene and its human homologue","volume":"372","author":"Zhang","year":"1994","journal-title":"Nature"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1006\/bbrc.1996.0587","article-title":"CDNA Cloning and expression of a novel adipose specific collagen-like factor, APM1 (AdiposeMost Abundant Gene Transcript 1)","volume":"221","author":"Maeda","year":"1996","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.diabres.2003.09.010","article-title":"Relationship between serum adiponectin and leptin concentrations and body fat distribution","volume":"63","author":"Park","year":"2004","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Riezu-Boj, J.I., Barajas, M., P\u00e9rez-S\u00e1nchez, T., Pajares, M.J., Ara\u00f1a, M., Milagro, F.I., and Urtasun, R. (2022). Lactiplantibacillus plantarum DSM20174 attenuates the progression of non-alcoholic fatty liver disease by modulating gut microbiota, improving metabolic risk factors, and attenuating adipose inflammation. Nutrients, 14.","DOI":"10.3390\/nu14245212"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s13679-018-0300-4","article-title":"Future pharmacotherapy for obesity: New anti-obesity drugs on the horizon","volume":"7","author":"Srivastava","year":"2018","journal-title":"Curr. Obes. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.cmet.2014.06.011","article-title":"A switch from white to brown fat increases energy expenditure in cancer-associated cachexia","volume":"20","author":"Petruzzelli","year":"2014","journal-title":"Cell Metab."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kang, J.H., Yun, S.I., Park, M.H., Park, J., Jeong, S.Y., and Park, H.O. (2013). Anti-obesity effect of Lactobacillus gasseri BNR17 in high-sucrose diet-induced obese mice. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0054617"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Abdulan, I.M., Popescu, G., Ma\u0219taleru, A., Oancea, A., Costache, A.D., Cojocaru, D.-C., Cump\u0103t, C.-M., Ciuntu, B.M., Rusu, B., and Leon, M.M. (2023). Winter holidays and their impact on eating behavior\u2014A systematic review. Nutrients, 15.","DOI":"10.3390\/nu15194201"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1192223","DOI":"10.3389\/fnut.2023.1192223","article-title":"Factors explaining seasonal variation in energy intake: A review","volume":"10","author":"Fujihira","year":"2023","journal-title":"Front. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Tanaka, N., Okuda, T., Shinohara, H., Yamasaki, R.S., Hirano, N., Kang, J., Ogawa, M., and Nishi, N.N. (2022). Relationship between seasonal changes in food intake and energy metabolism, physical activity, and body composition in young Japanese women. Nutrients, 14.","DOI":"10.3390\/nu14030506"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/raq.12033","article-title":"Probiotics in aquaculture: A current assessment","volume":"6","year":"2014","journal-title":"Rev. Aquacult."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1038\/ejcn.2010.19","article-title":"Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial","volume":"64","author":"Kadooka","year":"2010","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1002\/oby.21230","article-title":"Probiotic supplementation attenuates increases in body mass and fat mass during high-fat diet in healthy young adults","volume":"23","author":"Osterberg","year":"2015","journal-title":"Obesity"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Sung, H.K., Youn, S.J., Choi, Y., Eun, S.W., and Shin, S.M. (2023). Body fat reduction effect of Bifidobacterium breve B-3: A randomized, double-blind, placebo comparative clinical trial. Nutrients, 15.","DOI":"10.3390\/nu15010028"},{"key":"ref_37","first-page":"72","article-title":"SREBP-1c transcription factor and lipid homeostasis: Clinical perspective","volume":"68","author":"Ferre","year":"2007","journal-title":"Horm. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S1097-2765(00)80210-5","article-title":"PPAR gamma mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance","volume":"4","author":"Kubota","year":"1999","journal-title":"Mol. Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6207","DOI":"10.1073\/pnas.0306743102","article-title":"Deletion of PPARgamma in adipose tissues of mice protects against high fat diet-induced obesity and insulin resistance","volume":"102","author":"Jones","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1038\/sj.emboj.7601563","article-title":"Distinct C\/EBPa motifs regulate lipogenic and gluconeogenic gene expression in vivo","volume":"26","author":"Pedersen","year":"2007","journal-title":"EMBO J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5507","DOI":"10.1073\/pnas.93.11.5507","article-title":"The adipocyte specific transcription factor C\/EBPa modulates human ob gene expression","volume":"93","author":"Miller","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1016\/S0140-6736(12)60401-2","article-title":"Bariatric surgery for type 2 diabetes","volume":"379","author":"Dixon","year":"2012","journal-title":"Lancet"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/8\/1137\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:26:41Z","timestamp":1760106401000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/8\/1137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,11]]},"references-count":42,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["nu16081137"],"URL":"https:\/\/doi.org\/10.3390\/nu16081137","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,11]]}}}