{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T06:46:33Z","timestamp":1777185993976,"version":"3.51.4"},"reference-count":58,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tempus Public Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>An approach that shows promise for quickening the evolution of innovative anticancer drugs is the assessment of natural biomass sources. Our study sought to assess the effect of W. somnifera L. (WS) methanolic root and stem extracts on the expression of five targeted genes (cyclooxygenase-2, caspase-9, 5-Lipoxygenase, B-cell lymphoma-extra-large, and B-cell lymphoma 2) in colon cancer cell lines (Caco-2 cell lines). Plant extracts were prepared for bioassay by dissolving them in dimethyl sulfoxide. Caco-2 cell lines were exposed to various concentrations of plant extracts, followed by RNA extraction for analysis. By explicitly relating phytoconstituents of WS to the dose-dependent overexpression of caspase-9 genes and the inhibition of cyclooxygenase-2, 5-Lipoxygenase, B-cell lymphoma-extra-large, and B-cell lymphoma 2 genes, our novel findings characterize WS as a promising natural inhibitor of colorectal cancer (CRC) growth. Nonetheless, we recommend additional in vitro research to verify the current findings. With significant clinical benefits hypothesized, we offer WS methanolic root and stem extracts as potential organic antagonists for colorectal carcinogenesis and suggest further in vivo and clinical investigations, following successful in vitro trials. We recommend more investigation into the specific phytoconstituents in WS that contribute to the regulatory mechanisms that inhibit the growth of colon cancer cells.<\/jats:p>","DOI":"10.3390\/nu16081140","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T09:28:06Z","timestamp":1712914086000},"page":"1140","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Inhibition of Colorectal Cancer Gene Targets by Withania somnifera L. Methanolic Extracts: A Focus on Specific Genome Regulation"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7535-9478","authenticated-orcid":false,"given":"John M.","family":"Macharia","sequence":"first","affiliation":[{"name":"Doctoral School of Health Sciences, Faculty of Health Sciences, University of P\u00e9cs, V\u00f6r\u00f6smarty Mih\u00e1ly Str. 4, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel O.","family":"Pande","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Biomedical Science & Technology, School of Science and Applied Technology, Laikipia University, Nyahururu P.O. Box 1100-20300, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5656-089X","authenticated-orcid":false,"given":"Afshin","family":"Zand","sequence":"additional","affiliation":[{"name":"Department of Public Health Medicine, Medical School, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1562-9496","authenticated-orcid":false,"given":"Ferenc","family":"Bud\u00e1n","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Medical School, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsolt","family":"K\u00e1poszt\u00e1s","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2992-6503","authenticated-orcid":false,"given":"Orsolya","family":"K\u00f6vesdi","sequence":"additional","affiliation":[{"name":"Doctoral School of Health Sciences, Faculty of Health Sciences, University of P\u00e9cs, V\u00f6r\u00f6smarty Mih\u00e1ly Str. 4, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4950-0669","authenticated-orcid":false,"given":"T\u00edmea","family":"Varjas","sequence":"additional","affiliation":[{"name":"Department of Public Health Medicine, Medical School, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9551-8440","authenticated-orcid":false,"given":"Bence L.","family":"Raposa","sequence":"additional","affiliation":[{"name":"Institute of Basics of Health Sciences, Midwifery and Health Visiting, Faculty of Health Sciences, University of P\u00e9cs, V\u00f6r\u00f6smarty Mih\u00e1ly Str. 4, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"ref_1","first-page":"89","article-title":"Epidemiology of colorectal cancer: Incidence, mortality, survival, and risk factors","volume":"14","author":"Rawla","year":"2019","journal-title":"Prz. 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