{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:20Z","timestamp":1790553620850,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T00:00:00Z","timestamp":1712966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072190"],"award-info":[{"award-number":["32072190"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is a commonly encountered foodborne pathogen that can cause hemorrhagic enteritis and lead to hemolytic uremic syndrome (HUS) in severe cases. Bifidobacterium is a beneficial bacterium that naturally exists in the human gut and plays a vital role in maintaining a healthy balance in the gut microbiota. This study investigated the protective effects of B. longum K5 in a mouse model of EHEC O157:H7 infection. The results indicated that pretreatment with B. longum K5 mitigated the clinical symptoms of EHEC O157:H7 infection and attenuated the increase in myeloperoxidase (MPO) activity in the colon of the mice. In comparison to the model group, elevated serum D-lactic acid concentrations and diamine oxidase (DAO) levels were prevented in the K5-EHEC group of mice. The reduced mRNA expression of tight junction proteins (ZO-1, Occludin, and Claudin-1) and mucin MUC2, as well as the elevated expression of virulence factors Stx1A and Stx2A, was alleviated in the colon of both the K5-PBS and K5-EHEC groups. Additionally, the increase in the inflammatory cytokine levels of TNF-\u03b1 and IL-1\u03b2 was inhibited and the production of IL-4 and IL-10 was promoted in the K5-EHEC group compared with the model group. B. longum K5 significantly prevented the reduction in the abundance and diversity of mouse gut microorganisms induced by EHEC O157:H7 infection, including blocking the decrease in the relative abundance of Roseburia, Lactobacillus, and Oscillibacter. Meanwhile, the intervention with B. longum K5 promoted the production of acetic acid and butyric acid in the gut. This study provides insights into the use of B. longum K5 for developing probiotic formulations to prevent intestinal diseases caused by pathogenic bacterial infections.<\/jats:p>","DOI":"10.3390\/nu16081164","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T03:56:13Z","timestamp":1713153373000},"page":"1164","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Bifidobacterium longum K5 Prevents Enterohaemorrhagic Escherichia coli O157:H7 Infection in Mice through the Modulation of the Gut Microbiota"],"prefix":"10.3390","volume":"16","author":[{"given":"Deyu","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Cao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuli","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxue","family":"Yue","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8827-4409","authenticated-orcid":false,"given":"Fei","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guicheng","family":"Huo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bailiang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China"},{"name":"Food College, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1128\/CMR.00022-13","article-title":"Recent advances in understanding enteric pathogenic Escherichia coli","volume":"26","author":"Croxen","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Srutkova, D., Schwarzer, M., Hudcovic, T., Zakostelska, Z., Drab, V., Spanova, A., Rittich, B., Kozakova, H., and Schabussova, I. (2015). Bifidobacterium longum CCM 7952 promotes epithelial barrier function and prevents acute DSS-induced colitis in strictly strain-specific manner. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0134050"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Nguyen, Y., and Sperandio, V. (2012). Enterohemorrhagic E. coli (EHEC) pathogenesis. Front. Cell. Infect. Microbiol., 2.","DOI":"10.3389\/fcimb.2012.00090"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4014\/jmb.0908.08007","article-title":"A brief overview of Escherichia coli O157: H7 and its plasmid O157","volume":"20","author":"Lim","year":"2010","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Sharma, M., Wasan, A., and Sharma, R.K. (2021). Recent developments in probiotics: An emphasis on Bifidobacterium. Food Biosci., 41.","DOI":"10.1016\/j.fbio.2021.100993"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Jung, H.L., and Soon, H.S. (2020). Antimicrobial and Immunomodulatory Effects of Bifidobacterium Strains: A Review. J. Microbiol. Biotechnol., 30.","DOI":"10.4014\/jmb.2007.07046"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.anaerobe.2016.02.010","article-title":"Antagonistic activities of some Bifidobacterium sp. strains isolated from resident infant gastrointestinal microbiota on Gram-negative enteric pathogens","volume":"39","author":"Delcaru","year":"2016","journal-title":"Anaerobe"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nature09646","article-title":"Bifidobacteria can protect from enteropathogenic infection through production of acetate","volume":"469","author":"Fukuda","year":"2011","journal-title":"Nature"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Westermann, C., Gleinser, M., Corr, S.C., and Riedel, C.U. (2016). A critical evaluation of bifidobacterial adhesion to the host tissue. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01220"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jff.2018.12.022","article-title":"Colonization and probiotic function of Bifidobacterium longum","volume":"53","author":"Zhang","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s10482-009-9391-y","article-title":"Prevention of Escherichia coli O157: H7 infection in gnotobiotic mice associated with Bifidobacterium strains","volume":"97","author":"Yoshimura","year":"2010","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.tim.2011.10.001","article-title":"Microbial adhesins to gastrointestinal mucus","volume":"20","author":"Juge","year":"2012","journal-title":"Trends Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6463","DOI":"10.1007\/s00253-019-09978-7","article-title":"Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health","volume":"103","author":"Rastall","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhao, L., Wang, S., Dong, J., and Shi, J. (2021). Identification, Characterization, and Antioxidant Potential of Bifidobacterium longum subsp longum Strains Isolated From Feces of Healthy Infants. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.756519"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"105030","DOI":"10.1016\/j.jff.2022.105030","article-title":"Bifidobacterium longum subsp longum K5 alleviates inflammatory response and prevents intestinal barrier injury induced by LPS in vitro based on comparative genomics","volume":"92","author":"Zhao","year":"2022","journal-title":"J. Funct. Foods"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Dong, J., Ping, L., Xie, Q., Liu, D., Zhao, L., Evivie, S.E., Wang, Z., Li, B., and Huo, G. (2022). Lactobacillus plantarum KLDS1.0386 with antioxidant capacity ameliorates the lipopolysaccharide-induced acute liver injury in mice by NF-\u03baB and Nrf2 pathway. Food Biosci., 47.","DOI":"10.1016\/j.fbio.2022.101589"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5283","DOI":"10.1038\/s41598-021-84761-1","article-title":"DSS-induced colitis is associated with adipose tissue dysfunction and disrupted hepatic lipid metabolism leading to hepatosteatosis and dyslipidemia in mice","volume":"11","author":"Kwon","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"104715","DOI":"10.1016\/j.jff.2021.104715","article-title":"Effect of Bifidobacterium longum subsp. longum on the proliferative and tight-junction activities of Human Fetal Colon Epithelial Cells","volume":"86","author":"Jiaqi","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"104071","DOI":"10.1016\/j.jprot.2020.104071","article-title":"Distinct gut microbiota and metabolite profiles induced by delivery mode in healthy Chinese infants","volume":"232","author":"Na","year":"2021","journal-title":"J. Proteom."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1093\/bioinformatics\/btu494","article-title":"STAMP: Statistical analysis of taxonomic and functional profiles","volume":"30","author":"Parks","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5804","DOI":"10.1039\/C9FO01062A","article-title":"Lactobacillus acidophilus alleviates type 2 diabetes by regulating hepatic glucose, lipid metabolism and gut microbiota in mice","volume":"10","author":"Fenfen","year":"2019","journal-title":"Food Funct."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1078\/1438-4221-00235","article-title":"The role of intimin in the adherence of enterohaemorrhagic Escherichia coli (EHEC) O157: H7 to HEp-2 tissue culture cells and to bovine gut explant tissues","volume":"292","author":"Cookson","year":"2003","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1079\/AHR200463","article-title":"Escherichia coli 0157:H7: An update on intestinal colonization and virulence mechanisms","volume":"5","author":"Moxley","year":"2004","journal-title":"Anim. Health Res. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2217\/17460913.3.3.315","article-title":"Protective and destructive innate immune responses to enteropathogenic Escherichia coli and related A\/E pathogens","volume":"3","author":"Lebeis","year":"2008","journal-title":"Future Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"635","DOI":"10.2741\/A219","article-title":"Shiga toxin mode of action in E. coli O157:H7 disease","volume":"2","author":"Obrig","year":"1997","journal-title":"Front. Biosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5843","DOI":"10.1039\/C9FO01390C","article-title":"Lactobacillus casei LC2W can inhibit the colonization of Escherichia coli O157: H7 in vivo and reduce the severity of colitis","volume":"10","author":"Wang","year":"2019","journal-title":"Food Funct."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Bonvicini, F., Pagnotta, E., Punzo, A., Calabria, D., Simoni, P., Mirasoli, M., Passerini, N., Bertoni, S., Ugolini, L., and Lazzeri, L. (2020). Effect of Lactobacillus acidophilus fermented broths enriched with Eruca sativa seed extracts on intestinal barrier and inflammation in a co-culture system of an enterohemorrhagic Escherichia coli and human intestinal cells. Nutrients, 12.","DOI":"10.3390\/nu12103064"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"368","DOI":"10.5851\/kosfa.2017.37.3.368","article-title":"Beneficial effect of Bifidobacterium longum ATCC 15707 on survival rate of Clostridium difficile infection in mice","volume":"37","author":"Yun","year":"2017","journal-title":"Korean J. Food Sci. Anim. Resour."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Jiang, J., Wu, C., Zhang, C., Zhang, Q., Yu, L., Zhao, J., Zhang, H., Narbad, A., Chen, W., and Zhai, Q. (2021). Strain-specific effects of Bifidobacterium longum on hypercholesterolemic rats and potential mechanisms. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031305"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.nut.2019.02.005","article-title":"Supplementation with Bifidobacterium longum Bar33 and Lactobacillus helveticus Bar13 mixture improves immunity in elderly humans (over 75 years) and aged mice","volume":"63","author":"Finamore","year":"2019","journal-title":"Nutrition"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.biotechadv.2013.01.010","article-title":"Bacteriocin production by Bifidobacterium spp. A review","volume":"31","author":"Martinez","year":"2013","journal-title":"Biotechnol. Adv."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.idairyj.2005.09.006","article-title":"The in vitro inhibition of Gram-negative pathogenic bacteria by Bifidobacteria is caused by the production of organic acids","volume":"16","author":"Makras","year":"2006","journal-title":"Int. Dairy J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.aambs.2016.12.001","article-title":"Antivirulence properties of probiotics in combating microbial pathogenesis","volume":"98","author":"Nair","year":"2017","journal-title":"Adv. Appl. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s00284-016-1010-3","article-title":"Effect of Lactobacillus Strains on Intestinal Microflora and Mucosa Immunity in Escherichia coli O157:H7-Induced Diarrhea in Mice","volume":"73","author":"Bian","year":"2016","journal-title":"Curr. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ijfoodmicro.2006.04.041","article-title":"Effect of Bifidobacterium thermacidophilum probiotic feeding on enterohemorrhagic Escherichia coli O157:H7 infection in BALB\/c mice","volume":"111","author":"Ehab","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nri3608","article-title":"Intestinal epithelial cells: Regulators of barrier function and immune homeostasis","volume":"14","author":"Peterson","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e196","DOI":"10.1038\/ctg.2016.54","article-title":"Human intestinal barrier function in health and disease","volume":"7","author":"Wells","year":"2016","journal-title":"Clin. Transl. Gastroenterol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jaci.2009.05.038","article-title":"Intestinal barrier function: Molecular regulation and disease pathogenesis","volume":"124","author":"Groschwitz","year":"2009","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1111\/jam.14952","article-title":"Bacillus amyloliquefaciens TL106 protects mice against enterohaemorrhagic Escherichia coli O157: H7-induced intestinal disease through improving immune response, intestinal barrier function and gut microbiota","volume":"131","author":"Bao","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3390\/pathogens2010055","article-title":"Goblet cells and mucins: Role in innate defense in enteric infections","volume":"2","author":"Kim","year":"2013","journal-title":"Pathogens"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Gundamaraju, R., and Chong, W.C. (2021). Consequence of distinctive expression of MUC2 in colorectal cancers: How much is actually bad?. Biochim. Biophys. Acta BBA Rev. Cancer, 1876.","DOI":"10.1016\/j.bbcan.2021.188579"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/s41579-018-0036-x","article-title":"Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier","volume":"16","author":"Martens","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/j.ijbiomac.2020.07.191","article-title":"The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression","volume":"164","author":"Liu","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.fshw.2020.04.008","article-title":"The intervention effects of Lactobacillus casei LC2W on Escherichia coli O157: H7-induced mouse colitis","volume":"9","author":"Wang","year":"2020","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.micinf.2007.10.018","article-title":"Modulation of chemokine gene expression by Shiga-toxin producing Escherichia coli belonging to various origins and serotypes","volume":"10","author":"Gobert","year":"2008","journal-title":"Microbes Infect."},{"key":"ref_47","first-page":"112","article-title":"The role of inflammatory cytokines in bacterial infection","volume":"36","author":"Shi","year":"2009","journal-title":"Int. J. Intern. Med."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Zhao, X., Wang, L., Zhu, C., Xia, X., Zhang, S., Wang, Y., Zhang, H., Xu, Y., Chen, S., and Jiang, J. (2021). The antimicrobial peptide mastoparan X protects against enterohemorrhagic Escherichia coli O157: H7 infection, inhibits inflammation, and enhances the intestinal epithelial barrier. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.644887"},{"key":"ref_49","first-page":"175","article-title":"Inhibition of enterohemorrhagic Escherichia coli O157: H7 infection in a gnotobiotic mouse model with pre-colonization by Bacteroides strains","volume":"10","author":"Saito","year":"2019","journal-title":"Biomed. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2567.2012.03616.x","article-title":"Intestinal colonization resistance","volume":"138","author":"Lawley","year":"2013","journal-title":"Immunology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.it.2005.04.008","article-title":"Commensal gut bacteria: Mechanisms of immune modulation","volume":"26","author":"Kelly","year":"2005","journal-title":"Trends Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"104856","DOI":"10.1016\/j.micpath.2021.104856","article-title":"L. johnsonii, L. plantarum, and L. rhamnosus alleviated Enterohaemorrhagic Escherichia coli-induced diarrhoea in mice by regulating gut microbiota","volume":"154","author":"Hu","year":"2021","journal-title":"Microb. Pathog."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1194\/jlr.R036012","article-title":"The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism","volume":"54","author":"Groen","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/j.1365-2036.2007.03562.x","article-title":"The role of butyrate on colonic function","volume":"27","author":"Hamer","year":"2008","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3987","DOI":"10.1080\/10408398.2016.1203286","article-title":"Short chain and polyunsaturated fatty acids in host gut health and foodborne bacterial pathogen inhibition","volume":"57","author":"Peng","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1039\/C6FO01641C","article-title":"Bifidobacteria exert species-specific effects on constipation in BALB\/c mice","volume":"8","author":"Wang","year":"2017","journal-title":"Food Funct."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/8\/1164\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:27:39Z","timestamp":1760106459000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/8\/1164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,13]]},"references-count":56,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["nu16081164"],"URL":"https:\/\/doi.org\/10.3390\/nu16081164","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,13]]}}}