{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:39:10Z","timestamp":1791873550955,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T00:00:00Z","timestamp":1713139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish National Agency for Academic Exchange","award":["0102\/2019"],"award-info":[{"award-number":["0102\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: The occurrence of metabolic dysfunction-associated steatotic liver disease (MASLD) is a growing global problem which commonly affects patients with co-existing diseases\/conditions, such as type 2 diabetes and dyslipidemia. The effective treatment of MASLD is still limited; however, diet plays a significant role in its management. There are multiple beneficial properties of dietary fiber, including its ability to modify the gut microbiome. Therefore, the aim of this study was to determine the effect of the consumption of fiber-enriched rolls on the gut microbiome and microbial metabolites in patients suffering from MASLD. Methods: The participants were recruited according to the inclusion criteria and were required to consume fiber-enriched rolls containing either 6 g or 12 g of fiber. There were three assessment timepoints, when the anthropometric and laboratory parameters were measured, and 16s on nanopore sequencing of the fecal microbiome was conducted. Results: Firmicutes and Bacteroidetes were the most abundant phyla in the patients living with MASLD. It was demonstrated that the amount of short-chain fatty acids (SCFAs) changed after the consumption of fiber-enriched rolls; however, this was strongly associated with both the timepoint and the type of SCFAs\u2014acetate and butyrate. Additionally, the high-fiber diet was related to the increase in phyla diversity (p = 0.006571). Conclusions: Overall, the introduction of an appropriate amount of fiber to the diet seems to be promising for patients suffering from MASLD due to its ability to create an improvement in gut microbiome-related aspects.<\/jats:p>","DOI":"10.3390\/nu16081173","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T08:08:12Z","timestamp":1713168492000},"page":"1173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Gut Microbiome\u2014How Does Two-Month Consumption of Fiber-Enriched Rolls Change Microbiome in Patients Suffering from MASLD?"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0283-1436","authenticated-orcid":false,"given":"Karolina","family":"Ka\u017amierczak-Siedlecka","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Diagnostics\u2014Fahrenheit Biobank BBMRI.pl, Medical University of Gdansk, 80-211 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika","family":"Maciejewska-Markiewicz","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Metabolomics, Pomeranian Medical University, 71-460 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maciej","family":"Sykulski","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Medical University of Warsaw, 02-106 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Gruszczy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Herman-I\u017cycka","sequence":"additional","affiliation":[{"name":"GenXone S.A., ul. Kobaltowa 6, 62-002 Z\u0142otniki, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariusz","family":"Wyle\u017co\u0142","sequence":"additional","affiliation":[{"name":"Department of General, Vascular and Oncological Surgery, Medical University of Warsaw, 02-091 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Katarzyna Petriczko","sequence":"additional","affiliation":[{"name":"Translational Medicine Group, Pomeranian Medical University, 70-204 Szczecin, Poland"},{"name":"Department of Gastroenterology and Internal Medicine, SPWSZ Hospital, 71-455 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0620-3776","authenticated-orcid":false,"given":"Joanna","family":"Palma","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences, Pomeranian Medical University in Szczecin, Broniewskiego 24, 71-460 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5853-9709","authenticated-orcid":false,"given":"Karolina","family":"Jakubczyk","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Metabolomics, Pomeranian Medical University, 71-460 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4548-3419","authenticated-orcid":false,"given":"Katarzyna","family":"Janda-Milczarek","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Metabolomics, Pomeranian Medical University, 71-460 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3430-9079","authenticated-orcid":false,"given":"Karolina","family":"Skonieczna-\u017bydecka","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences, Pomeranian Medical University in Szczecin, Broniewskiego 24, 71-460 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4009-1977","authenticated-orcid":false,"given":"Ewa","family":"Stachowska","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Metabolomics, Pomeranian Medical University, 71-460 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.jhep.2023.07.029","article-title":"Uniting to defeat steatotic liver disease: A global mission to promote healthy livers and healthy lives","volume":"79","author":"Krag","year":"2023","journal-title":"J. Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1097\/HEP.0000000000000004","article-title":"The global epidemiology of nonalcoholic fatty liver disease (MASLD) and nonalcoholic steatohepatitis (NASH): A systematic review","volume":"77","author":"Younossi","year":"2023","journal-title":"Hepatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"16","DOI":"10.21037\/tgh.2019.09.08","article-title":"Epidemiology of non-alcoholic and alcoholic fatty liver diseases","volume":"5","author":"Mitra","year":"2020","journal-title":"Transl. Gastroenterol. Hepatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/s41591-018-0104-9","article-title":"Mechanisms of NAFLD development and therapeutic strategies","volume":"24","author":"Friedman","year":"2018","journal-title":"Nat. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1136\/gutjnl-2020-323106","article-title":"Effect of green-Mediterranean diet on intrahepatic fat: The DIRECT PLUS randomised controlled trial","volume":"70","author":"Rinott","year":"2021","journal-title":"Gut"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1038\/s41430-020-0642-3","article-title":"Prevalence of malnutrition and analysis of related factors in elderly patients with COVID-19 in Wuhan, China","volume":"74","author":"Li","year":"2020","journal-title":"Eur. J Clin. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Meroni, M., Longo, M., Rustichelli, A., and Dongiovanni, P. (2020). Nutrition and Genetics in NAFLD: The Perfect Binomium. Int. J Mol. Sci., 21.","DOI":"10.3390\/ijms21082986"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Abenavoli, L., Boccuto, L., Federico, A., Dallio, M., Loguercio, C., Di Renzo, L., and De Lorenzo, A. (2019). Diet and Non-Alcoholic Fatty Liver Disease: The Mediterranean Way. Int. J. Environ. Res. Public Health, 16.","DOI":"10.3390\/ijerph16173011"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4427","DOI":"10.1210\/jc.2019-00481","article-title":"Effect of Prebiotic on Microbiota, Intestinal Permeability, and Glycemic Control in Children With Type 1 Diabetes","volume":"104","author":"Ho","year":"2019","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ojo, O., Ojo, O.O., Zand, N., and Wang, X. (2021). The Effect of Dietary Fibre on Gut Microbiota, Lipid Profile, and Inflammatory Markers in Patients with Type 2 Diabetes: A Systematic Review and Meta-Analysis of Randomised Controlled Trials. Nutrients, 13.","DOI":"10.3390\/nu13061805"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Stachowska, E., Portincasa, P., Jamio\u0142-Milc, D., Maciejewska-Markiewicz, D., and Skonieczna-\u017bydecka, K. (2020). The Relationship between Prebiotic Supplementation and Anthropometric and Biochemical Parameters in Patients with NAFLD-A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Nutrients, 12.","DOI":"10.3390\/nu12113460"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1079\/PHN2005898","article-title":"The International Physical Activity Questionnaire (IPAQ): A study of concurrent and construct validity","volume":"9","author":"Oja","year":"2006","journal-title":"Public Health Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1017\/S1368980011002515","article-title":"Mediterranean diet pyramid today. Science and cultural up-dates","volume":"14","author":"Berry","year":"2011","journal-title":"Public Health Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Karlas, T., Petroff, D., Garnov, N., B\u00f6hm, S., Tenckhoff, H., Wittekind, C., Wiese, M., Schiefke, I., Linder, N., and Schaudinn, A. (2014). Non-invasive assessment of hepatic steatosis in patients with NAFLD using con-trolled attenuation parameter and 1H-MR spectroscopy. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0091987"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1017\/S1368980019001022","article-title":"Relative validation of the adapted Mediterranean Diet Score for Adolescents by comparison with nutritional biomarkers and nutrient and food in-takes: The Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA) study","volume":"22","author":"Moreno","year":"2019","journal-title":"Public Health Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1007\/s00018-019-03011-w","article-title":"The links between the gut microbiome and non-alcoholic fatty liver disease (NAFLD)","volume":"76","author":"Safari","year":"2019","journal-title":"Cell Mol. Life Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Jamio\u0142-Milc, D., Gudan, A., Ka\u017amierczak-Siedlecka, K., Ho\u0142owko-Zi\u00f3\u0142ek, J., Maciejewska-Markiewicz, D., Janda-Milczarek, K., and Stachowska, E. (2023). Nutritional Support for Liver Diseases. Nutrients, 15.","DOI":"10.3390\/nu15163640"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1111\/liv.15024","article-title":"Diet and exercise in NAFLD\/NASH: Beyond the obvious","volume":"41","author":"Semmler","year":"2021","journal-title":"Liver Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1016\/j.clnu.2022.06.037","article-title":"The effectiveness and acceptability of Mediterranean diet and calorie restriction in non-alcoholic fatty liver dis-ease (NAFLD): A systematic review and meta-analysis","volume":"41","author":"Haigh","year":"2022","journal-title":"Clin. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Montes de Oca, A., Juli\u00e1n, M.T., Ramos, A., Puig-Domingo, M., and Alonso, N. (2020). Microbiota, Fiber, and NAFLD: Is There Any Connection?. Nutrients, 12.","DOI":"10.3390\/nu12103100"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1080\/19490976.2017.1290756","article-title":"Dietary fiber and prebiotics and the gastrointestinal microbiota","volume":"8","author":"Holscher","year":"2017","journal-title":"Gut Microbes"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Usuda, H., Okamoto, T., and Wada, K. (2021). Leaky Gut: Effect of Dietary Fiber and Fats on Microbiome and Intestinal Barrier. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22147613"},{"key":"ref_23","first-page":"1443","article-title":"Microbiota-derived metabolites in colorectal cancer patients in preoperative period","volume":"27","author":"Palma","year":"2023","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1530\/JOE-20-0018","article-title":"SCFAs alleviated steatosis and inflammation in mice with NASH induced by MCD","volume":"245","author":"Deng","year":"2020","journal-title":"J. Endocrinol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e2200597","DOI":"10.1002\/mnfr.202200597","article-title":"Butyrate Attenuates Hepatic Steatosis Induced by a High-Fat and Fiber-Deficient Diet via the Hepatic GPR41\/43-CaMKII\/HDAC1-CREB Pathway","volume":"67","author":"Zheng","year":"2023","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1177\/2050640618804444","article-title":"Fecal SCFAs and SCFA-producing bacteria in gut microbiome of human NAFLD as a putative link to systemic T-cell activation and advanced disease","volume":"6","author":"Rau","year":"2018","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.hbpd.2023.06.006","article-title":"Gut microbiome and nonalcoholic fatty liver disease","volume":"22","author":"Wu","year":"2023","journal-title":"Hepatobiliary Pancreat. Dis. Int."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chen, J., and Vitetta, L. (2020). Gut Microbiota Metabolites in NAFLD Pathogenesis and Therapeutic Implications. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155214"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ji, Y., Yin, Y., Li, Z., and Zhang, W. (2019). Gut Microbiota-Derived Components and Metabolites in the Progression of Non-Alcoholic Fatty Liver Disease (NAFLD). Nutrients, 11.","DOI":"10.3390\/nu11081712"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Hrncir, T., Hrncirova, L., Kverka, M., Hromadka, R., Machova, V., Trckova, E., Kostovcikova, K., Kral-ickova, P., Krejsek, J., and Tlaskalova-Hogenova, H. (2021). Gut Microbiota and NAFLD: Pathogenetic Mechanisms, Microbiota Signatures, and Therapeutic Interventions. Microorganisms, 9.","DOI":"10.3390\/microorganisms9050957"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1111\/cas.15142","article-title":"Gut-liver axis-mediated mechanism of liver cancer: A special focus on the role of gut microbiota","volume":"112","author":"Ohtani","year":"2021","journal-title":"Cancer Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"678360","DOI":"10.3389\/fimmu.2021.678360","article-title":"Prebiotic Inulin and Sodium Butyrate Attenuate Obesity-Induced Intestinal Barrier Dysfunction by Induction of Antimicrobial Peptides","volume":"12","author":"Beisner","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3611","DOI":"10.1111\/1750-3841.15367","article-title":"Effects of probiotic and prebiotic supplementation on metabolic parameters, liver aminotransferases, and systemic inflammation in nonalcoholic fatty liver disease: A randomized clinical trial","volume":"85","author":"Behrouz","year":"2020","journal-title":"J. Food Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"eabk0855","DOI":"10.1126\/scitranslmed.abk0855","article-title":"Risk assessment with gut microbiome and metabolite markers in NAFLD development","volume":"14","author":"Leung","year":"2022","journal-title":"Sci. Transl. Med."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Vallianou, N., Christodoulatos, G.S., Karampela, I., Tsilingiris, D., Magkos, F., Stratigou, T., Kounatidis, D., and Dalamaga, M. (2021). Understanding the Role of the Gut Microbiome and Microbial Metabolites in Non-Alcoholic Fatty Liver Disease: Current Evidence and Perspectives. Biomolecules, 12.","DOI":"10.3390\/biom12010056"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e13768","DOI":"10.1111\/eci.13768","article-title":"The role of microbiota in nonalcoholic fatty liver disease","volume":"52","author":"Bonfrate","year":"2022","journal-title":"Eur. J Clin. Investig."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1161\/CIRCULATIONAHA.116.024545","article-title":"High-Fiber Diet and Acetate Supplementation Change the Gut Microbiota and Prevent the Development of Hypertension and Heart Failure in Hypertensive Mice","volume":"135","author":"Marques","year":"2017","journal-title":"Circulation"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"50","DOI":"10.12938\/bmfh.2020-046","article-title":"Correlation of gut Firmicutes\/Bacteroidetes ratio with fibrosis and steatosis stratified by body mass index in patients with non-alcoholic fatty liver disease","volume":"40","author":"Jasirwan","year":"2021","journal-title":"Biosci. Microbiota Food Health"},{"key":"ref_39","first-page":"483","article-title":"Impact of a low-carbohydrate and high-fiber diet on nonalcoholic fatty liver disease","volume":"29","author":"Chen","year":"2020","journal-title":"Asia Pac. J. Clin. Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1038435","DOI":"10.3389\/fpubh.2022.1038435","article-title":"Dietary fiber intake and non-alcoholic fatty liver disease: The mediating role of obesity","volume":"10","author":"Zhu","year":"2022","journal-title":"Front. Public Health"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/8\/1173\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:28:11Z","timestamp":1760106491000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/8\/1173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,15]]},"references-count":40,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["nu16081173"],"URL":"https:\/\/doi.org\/10.3390\/nu16081173","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,15]]}}}