{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:13:15Z","timestamp":1790194395873,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T00:00:00Z","timestamp":1714003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Major Science and Technology Project of the Science and Technology Department of Yunnan Province","award":["202102AE090050"],"award-info":[{"award-number":["202102AE090050"]}]},{"name":"Major Science and Technology Project of the Science and Technology Department of Yunnan Province","award":["202202AG050009"],"award-info":[{"award-number":["202202AG050009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Distillers\u2019 grains are rich in protein and constitute a high-quality source of various bioactive peptides. The purpose of this study is to identify novel bioactive peptides with \u03b1-glucosidase inhibitory, antioxidant, and insulin resistance-ameliorating effects from distiller\u2019s grains protein hydrolysate. Three novel peptides (YPLPR, AFEPLR, and NDPF) showed good potential bioactivities, and the YPLPR peptide had the strongest bioactivities, whose IC50 values towards \u03b1-glucosidase inhibition, radical scavenging rates of 2,2\u2032-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were about 5.31 mmol\/L, 6.05 mmol\/L, and 7.94 mmol\/L, respectively. The glucose consumption of HepG2 cells treated with YPLPR increased significantly under insulin resistance condition. Moreover, the YPLPR peptide also had a good scavenging effect on intracellular reactive oxygen species (ROS) induced by H2O2 (the relative contents: 102.35% vs. 100%). Molecular docking results showed that these peptides could stably combine with \u03b1-glucosidase, ABTS, and DPPH free radicals, as well as related targets of the insulin signaling pathway through hydrogen bonding and van der Waals forces. This research presents a potentially valuable natural resource for reducing oxidative stress damage and regulating blood glucose in diabetes, thereby increasing the usage of distillers\u2019 grains peptides and boosting their economic worth.<\/jats:p>","DOI":"10.3390\/nu16091279","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T08:08:32Z","timestamp":1714032512000},"page":"1279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Identification of Novel Peptides in Distillers\u2019 Grains as Antioxidants, \u03b1-Glucosidase Inhibitors, and Insulin Sensitizers: In Silico and In Vitro Evaluation"],"prefix":"10.3390","volume":"16","author":[{"given":"Lixin","family":"Ding","sequence":"first","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"},{"name":"Yunnan Engineering Research Center for Fruit & Vegetable Products, Kunming 650500, China"},{"name":"International Green Food Processing Research and Development Center of Kunming City, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuqing","family":"Zheng","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"},{"name":"Yunnan Engineering Research Center for Fruit & Vegetable Products, Kunming 650500, China"},{"name":"International Green Food Processing Research and Development Center of Kunming City, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2492-5062","authenticated-orcid":false,"given":"Shengbao","family":"Cai","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"},{"name":"Yunnan Engineering Research Center for Fruit & Vegetable Products, Kunming 650500, China"},{"name":"International Green Food Processing Research and Development Center of Kunming City, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s11886-019-1107-y","article-title":"Epidemiology of Diabetes Mellitus and Cardiovascular Disease","volume":"21","author":"Glovaci","year":"2019","journal-title":"Curr. 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