{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T17:18:09Z","timestamp":1794071889295,"version":"4.8.0"},"reference-count":81,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Aalborg University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Vaginally administered postbiotics derived from Lactobacillus were recently demonstrated to be effective in alleviating bacterial vaginosis and increasing pregnancy rates. However, their potential effect on sperm quality has not been well investigated. This controlled in vitro study aimed to assess the dose- and time-dependent effects of postbiotics derived from Lactobacillus\u00a0rhamnosus PB01 (DSM 14870) on sperm quality parameters. The experiment was conducted in vitro to eliminate potential confounding factors from the female reproductive tract and vaginal microbiota. Sperm samples from 18 healthy donors were subjected to analysis using Computer-Aided Sperm Analysis (CASA) in various concentrations of postbiotics and control mediums at baseline, 60 min, and 90 min of incubation. Results indicated that lower postbiotic concentration (PB5) did not adversely affect sperm motility, kinematic parameters, sperm DNA fragmentation, and normal morphology at any time. However, concentrations exceeding 15% demonstrated a reduction in progressively motile sperm and a negative correlation with non-progressively motile sperm at all time points. These findings underscore the importance of balancing postbiotic dosage to preserve sperm motility while realizing the postbiotics\u2019 vaginal health benefits. Further research is warranted to understand the underlying mechanisms and refine practical applications in reproductive health.<\/jats:p>","DOI":"10.3390\/nu16111781","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T03:44:35Z","timestamp":1717645475000},"page":"1781","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of Postbiotics Derived from Lactobacillus\u00a0rhamnosus PB01 (DSM 14870) on Sperm Quality: A Prospective In Vitro Study"],"prefix":"10.3390","volume":"16","author":[{"given":"Sihan","family":"Liu","sequence":"first","affiliation":[{"name":"Regenerative Medicine Group, Department of Health Science and Technology, Aalborg University, 9260 Gistrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8500-4208","authenticated-orcid":false,"given":"Hiva","family":"Alipour","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Department of Health Science and Technology, Aalborg University, 9260 Gistrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5279-2690","authenticated-orcid":false,"given":"Vladimir","family":"Zachar","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Department of Health Science and Technology, Aalborg University, 9260 Gistrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrik Schi\u00f8ler","family":"Kesmodel","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Aalborg University, 9260 Gistrup, Denmark"},{"name":"Department of Obstetrics and Gynecology, Aalborg University Hospital, 9000 Aalborg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Dardmeh","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Department of Health Science and Technology, Aalborg University, 9260 Gistrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"129628","DOI":"10.3389\/fphys.2015.00081","article-title":"Lactobacillus Species as Biomarkers and Agents That Can Promote Various Aspects of Vaginal Health","volume":"6","author":"Petrova","year":"2015","journal-title":"Front. 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