{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:16:27Z","timestamp":1781630187096,"version":"3.54.5"},"reference-count":77,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["956279"],"award-info":[{"award-number":["956279"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["100010434"],"award-info":[{"award-number":["100010434"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["LCF\/BQ\/PR21\/11840001"],"award-info":[{"award-number":["LCF\/BQ\/PR21\/11840001"]}]},{"name":"la Caixa Foundation","award":["956279"],"award-info":[{"award-number":["956279"]}]},{"name":"la Caixa Foundation","award":["100010434"],"award-info":[{"award-number":["100010434"]}]},{"name":"la Caixa Foundation","award":["LCF\/BQ\/PR21\/11840001"],"award-info":[{"award-number":["LCF\/BQ\/PR21\/11840001"]}]},{"name":"Junior Leader Fellowship","award":["956279"],"award-info":[{"award-number":["956279"]}]},{"name":"Junior Leader Fellowship","award":["100010434"],"award-info":[{"award-number":["100010434"]}]},{"name":"Junior Leader Fellowship","award":["LCF\/BQ\/PR21\/11840001"],"award-info":[{"award-number":["LCF\/BQ\/PR21\/11840001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Enterohemorrhagic Escherichia coli (EHEC) is a major food-borne pathogen that causes human disease ranging from diarrhea to life-threatening complications. Accumulating evidence demonstrates that the Western diet enhances the susceptibility to enteric infection in mice, but the effect of diet on EHEC colonization and the role of human gut microbiota remains unknown. Our research aimed to investigate the effects of a Standard versus a Western diet on EHEC colonization in the human in vitro Mucosal ARtificial COLon (M-ARCOL) and the associated changes in the gut microbiota composition and activities. After donor selection using simplified fecal batch experiments, two M-ARCOL bioreactors were inoculated with a human fecal sample (n = 4) and were run in parallel, one receiving a Standard diet, the other a Western diet and infected with EHEC O157:H7 strain EDL933. EHEC colonization was dependent on the donor and diet in the luminal samples, but was maintained in the mucosal compartment without elimination, suggesting a favorable niche for the pathogen, and may act as a reservoir. The Western diet also impacted the bacterial short-chain fatty acid and bile acid profiles, with a possible link between high butyrate concentrations and prolonged EHEC colonization. The work demonstrates the application of a complex in vitro model to provide insights into diet, microbiota, and pathogen interactions in the human gut.<\/jats:p>","DOI":"10.3390\/nu16132046","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T08:57:50Z","timestamp":1719478670000},"page":"2046","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Western Diet on Enterohemorrhagic Escherichia coli Colonization in the Human In Vitro Mucosal Artificial Colon as Mediated by Gut Microbiota"],"prefix":"10.3390","volume":"16","author":[{"given":"Deborah","family":"O\u2019Sullivan","sequence":"first","affiliation":[{"name":"UMR 454 INRAe, Microbiology, Digestive Environment and Health (MEDIS), Universit\u00e9 Clermont Auvergne, 28 Place Henri Dunant, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trisha","family":"Arora","sequence":"additional","affiliation":[{"name":"Centre for Omics Sciences (COS), Unique Scientific and Technical Infrastructures (ICTS), Eurecat\u2014Technology Centre of Catalonia & Rovira i Virgili University Joint Unit, 43204 Reus, Spain"},{"name":"Department of Electrical, Electronic and Control Engineering (DEEEA), Universitat Rovira i Virgili, 43007 Tarragona, Spain"},{"name":"Computational Metabolomics for Systems Biology Lab, Eurecat-Technology Centre of Catalonia, 08005 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9406-1458","authenticated-orcid":false,"given":"Claude","family":"Durif","sequence":"additional","affiliation":[{"name":"UMR 454 INRAe, Microbiology, Digestive Environment and Health (MEDIS), Universit\u00e9 Clermont Auvergne, 28 Place Henri Dunant, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oph\u00e9lie","family":"Uriot","sequence":"additional","affiliation":[{"name":"UMR 454 INRAe, Microbiology, Digestive Environment and Health (MEDIS), Universit\u00e9 Clermont Auvergne, 28 Place Henri Dunant, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgane","family":"Brun","sequence":"additional","affiliation":[{"name":"UMR 454 INRAe, Microbiology, Digestive Environment and Health (MEDIS), Universit\u00e9 Clermont Auvergne, 28 Place Henri Dunant, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9416-7116","authenticated-orcid":false,"given":"Marc","family":"Riu","sequence":"additional","affiliation":[{"name":"Centre for Omics Sciences (COS), Unique Scientific and Technical Infrastructures (ICTS), Eurecat\u2014Technology Centre of Catalonia & Rovira i Virgili University Joint Unit, 43204 Reus, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabet","family":"Foguet-Romero","sequence":"additional","affiliation":[{"name":"Centre for Omics Sciences (COS), Unique Scientific and Technical Infrastructures (ICTS), Eurecat\u2014Technology Centre of Catalonia & Rovira i Virgili University Joint Unit, 43204 Reus, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-6889","authenticated-orcid":false,"given":"Iris","family":"Samarra","sequence":"additional","affiliation":[{"name":"Centre for Omics Sciences (COS), Unique Scientific and Technical Infrastructures (ICTS), Eurecat\u2014Technology Centre of Catalonia & Rovira i Virgili University Joint Unit, 43204 Reus, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0133-6863","authenticated-orcid":false,"given":"Xavier","family":"Domingo-Almenara","sequence":"additional","affiliation":[{"name":"Centre for Omics Sciences (COS), Unique Scientific and Technical Infrastructures (ICTS), Eurecat\u2014Technology Centre of Catalonia & Rovira i Virgili University Joint Unit, 43204 Reus, Spain"},{"name":"Department of Electrical, Electronic and Control Engineering (DEEEA), Universitat Rovira i Virgili, 43007 Tarragona, Spain"},{"name":"Computational Metabolomics for Systems Biology Lab, Eurecat-Technology Centre of Catalonia, 08005 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cormac G. M.","family":"Gahan","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland"},{"name":"School of Microbiology, University College Cork, T12 K8AF Cork, Ireland"},{"name":"School of Pharmacy, University College Cork, T12 K8AF Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6104-6043","authenticated-orcid":false,"given":"Lucie","family":"Etienne-Mesmin","sequence":"additional","affiliation":[{"name":"UMR 454 INRAe, Microbiology, Digestive Environment and Health (MEDIS), Universit\u00e9 Clermont Auvergne, 28 Place Henri Dunant, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Blanquet-Diot","sequence":"additional","affiliation":[{"name":"UMR 454 INRAe, Microbiology, Digestive Environment and Health (MEDIS), Universit\u00e9 Clermont Auvergne, 28 Place Henri Dunant, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4930","DOI":"10.1128\/JCM.41.11.4930-4940.2003","article-title":"Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and\/or serious disease","volume":"41","author":"Karmali","year":"2003","journal-title":"J. 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