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Increasing antibiotic resistance levels are a major global concern and alternative treatments are needed. Soybean peptides and other compounds might be an alternative in the treatment to avoid, eradicate and\/or control symptoms of H. pylori infection. This study aimed to characterize a lunasin-enriched soybean extract (LSE) using proteomics tools and to evaluate its antioxidant, anti-inflammatory and antibacterial properties against H. pylori infection. By LC\u2013MS\/MS analysis, 124 proteins were identified, with 2S albumin (lunasin and large-chain subunits) being the fourth most abundant protein (8.9%). Lunasin consists of 44 amino acid residues and an intramolecular disulfide bond. LSE at a low dose (0.0625 mg\/mL) reduced ROS production in both H. pylori-infected and non-infected AGS gastric cells. This led to a significant reduction of 6.71% in the levels of pro-inflammatory interleukin (IL)-8. LSE also showed antibacterial activity against H. pylori, which can be attributed to other soybean proteins and phenolic compounds. Our findings suggest that LSE might be a promising alternative in the management of H. pylori infection and its associated symptoms.<\/jats:p>","DOI":"10.3390\/nu16132056","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T13:26:43Z","timestamp":1719494803000},"page":"2056","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proteomic Characterization of a Lunasin-Enriched Soybean Extract Potentially Useful in the Treatment of Helicobacter pylori Infection"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3906-1607","authenticated-orcid":false,"given":"Giselle","family":"Franca-Oliveira","sequence":"first","affiliation":[{"name":"Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicol\u00e1s Cabrera 9, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Monreal Peinado","sequence":"additional","affiliation":[{"name":"Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicol\u00e1s Cabrera 9, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5330-8370","authenticated-orcid":false,"given":"Stephanny Miranda","family":"Alves de Souza","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Nanobiossistemas, Universidade Federal do Rio de Janeiro, Duque de Caxias 25240-005, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4194-7404","authenticated-orcid":false,"given":"Dario Eluan","family":"Kalume","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Interdisciplinar de Pesquisas M\u00e9dicas, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21041-250, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0109-0357","authenticated-orcid":false,"given":"Theo Luiz","family":"Ferraz de Souza","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Nanobiossistemas, Universidade Federal do Rio de Janeiro, Duque de Caxias 25240-005, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7192-6044","authenticated-orcid":false,"given":"Blanca","family":"Hern\u00e1ndez-Ledesma","sequence":"additional","affiliation":[{"name":"Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicol\u00e1s Cabrera 9, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6140-0732","authenticated-orcid":false,"given":"Adolfo J.","family":"Martinez-Rodriguez","sequence":"additional","affiliation":[{"name":"Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicol\u00e1s Cabrera 9, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1053\/j.gastro.2017.04.022","article-title":"Global prevalence of Helicobacter pylori infection: Systematic review and meta-analysis","volume":"153","author":"Hooi","year":"2017","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"D\u00edaz, P., Valenzuela Valderrama, M., Bravo, J., and Quest, A.F.G. 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