{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:56:20Z","timestamp":1792036580413,"version":"4.4.0"},"reference-count":51,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302096"],"award-info":[{"award-number":["32302096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["63241340"],"award-info":[{"award-number":["63241340"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["63241341"],"award-info":[{"award-number":["63241341"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Fundamental Research Funds for the Central Universities, Nankai University","award":["32302096"],"award-info":[{"award-number":["32302096"]}]},{"name":"the Fundamental Research Funds for the Central Universities, Nankai University","award":["63241340"],"award-info":[{"award-number":["63241340"]}]},{"name":"the Fundamental Research Funds for the Central Universities, Nankai University","award":["63241341"],"award-info":[{"award-number":["63241341"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Alcoholic liver damage is caused by long-term or heavy drinking, and it may further progress into alcoholic liver diseases (ALD). Probiotic supplements have been suggested for the prevention or improvement of liver damage. This study was designed to consider the ameliorative effects of Lactobacillus rhamnosus NKU FL1-8 isolated from infant feces against alcoholic liver damage. The mice were gavaged with a 50% ethanol solution and treated with 109 CFU of L. rhamnosus NKU FL1-8 suspension. The factors for liver function, oxidative stress, inflammation, gut microbiota composition, and intestinal barrier integrity were measured. The results showed that L. rhamnosus NKU FL1-8 could decrease the levels of aspartate aminotransferase (AST) to 61% and alanine aminotransferase (ALT) to 50% compared with ethanol given by gavage. It could inhibit the expression level of malondialdehyde (MDA), increase superoxide dismutase (SOD), glutathione (GSH) to relieve oxidative stress, and down-regulate the cytokines to decrease hepatic inflammation. After treatment, the level of triglycerides was reduced, and the expression levels of adenosine 5\u2032-monophosphate (AMP)-activated protein kinase (AMPK) and the peroxisome proliferators-activated receptor-\u03b1 (PPAR-\u03b1) pathway were up-regulated. Additionally, the 16S rRNA sequencing analysis showed that L. rhamnosus NKU FL1-8 increased the relative abundance of Lactobacillus, Ruminococcaceae, etc. At the same time, L. rhamnosus NKU FL1-8 could significantly reduce lipopolysaccharides (LPS) and enhance intestinal tight junction proteins. These results demonstrated that L. rhamnosus NKU FL1-8 could reduce the level of oxidative stress, fat accumulation, and liver inflammation caused by alcohol in the host. The underlying mechanism could be that L. rhamnosus NKU FL1-8 inhibits LPS by regulating the gut microbiota and repairing the intestinal barrier. Thereby, these findings support L. rhamnosus NKU FL1-8 as a potential functional food for the relief of ALD.<\/jats:p>","DOI":"10.3390\/nu16132139","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T09:50:32Z","timestamp":1720086632000},"page":"2139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Lactobacillus rhamnosus NKU FL1-8 Isolated from Infant Feces Ameliorates the Alcoholic Liver Damage by Regulating the Gut Microbiota and Intestinal Barrier in C57BL\/6J Mice"],"prefix":"10.3390","volume":"16","author":[{"given":"Haiwei","family":"Liu","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1707-1916","authenticated-orcid":false,"given":"Dancai","family":"Fan","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2131-0676","authenticated-orcid":false,"given":"Yuanyifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sihao","family":"Wu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingmin","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1016\/S0140-6736(12)61728-0","article-title":"Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: A systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"Lozano","year":"2012","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1080\/13548506.2018.1467025","article-title":"Binge drinking among young adults in Israel: Application of the theory of planned behavior","volume":"23","author":"Lawental","year":"2018","journal-title":"Psychol. 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