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The regulation of NAFLD through plant-derived active substances has become a new area of interest. Quinoa (Chenopodium quinoa Willd.) has been discovered to contain a large quantity of bioactive compounds. In this study, we established a free fatty acid (FFA)-induced steatosis model and explored the effects of quinoa polyphenol extract (QPE) on the major hallmarks of NAFLD. The results indicated that QPE significantly reduced intracellular triglyceride (TG) and total cholesterol (TC) levels. Additionally, QPE remarkably elevated the levels of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) and lowered levels of malondialdehyde (MDA). Further examination revealed that QPE attenuated intracellular inflammation, which was verified by the reduced levels of pro-inflammatory cytokines. Mechanistically, QPE inhibited fatty acid biosynthesis mainly by targeting de novo lipogenesis (DNL) via the AMPK\/SREBP-1c signaling pathway. Moreover, network pharmacology was used to analyze key targets for NAFLD mitigation by ferulic acid (FA), a major component of QPE. Taken together, this study suggests that QPE could ameliorate NAFLD by modulating hepatic lipid metabolism and alleviating oxidative stress and inflammation.<\/jats:p>","DOI":"10.3390\/nu16142276","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T12:44:36Z","timestamp":1721047476000},"page":"2276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Quinoa Polyphenol Extract Alleviates Non-Alcoholic Fatty Liver Disease via Inhibiting Lipid Accumulation, Inflammation and Oxidative Stress"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9264-5286","authenticated-orcid":false,"given":"Wenjun","family":"Yao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Wuxi 214026, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcong","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Wuxi 214026, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Qian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Wuxi 214026, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9402-1093","authenticated-orcid":false,"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Wuxi 214026, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Wuxi 214026, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"key":"ref_1","first-page":"1521","article-title":"Nonalcoholic fatty liver disease: A systematic review","volume":"314","author":"Rinella","year":"2015","journal-title":"JAMA-J. 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