{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T21:19:25Z","timestamp":1780694365602,"version":"3.54.1"},"reference-count":48,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T00:00:00Z","timestamp":1722643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Centre in Poland","award":["2015\/17\/B\/NZ9\/01740"],"award-info":[{"award-number":["2015\/17\/B\/NZ9\/01740"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Male infertility represents a significant public health concern. There is a negative impact of inflammatory bowel diseases (IBDs) on the male reproductive system. The aim of this study was to investigate whether oat beta-glucan (OBG) with different molar mass can modulate parameters of antioxidant defense and inflammatory response in the testes of adult Sprague\u2013Dawley rats with TNBS-induced colitis and whether the OBG intervention can modulate the inflammatory response in association with the RAS system. Results: higher testicular superoxide dismutase (SOD), glutathione reductase (GR) activities and glutathione (GSH) concentration, and lower testosterone (T) level and glutathione peroxidase (GPx) activity, were observed in rats with colitis than in healthy control ones. TNBS-induced colitis resulted in decreased the angiotensin 1\u20137 (ANG 1\u20137) level in the testes of rats fed with low-molar mass OBG compared to control animals. Conclusions: although colitis induced moderate pro-oxidant changes in the gonads, it seems plausible that dietary intervention with different fractions of oat beta-glucans mass may support the maintenance of reproductive homeostasis via the stimulation of the local antioxidant defense system.<\/jats:p>","DOI":"10.3390\/nu16152546","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T11:28:54Z","timestamp":1722857334000},"page":"2546","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Oat Beta-Glucan Dietary Intervention on Antioxidant Defense Parameters, Inflammatory Response and Angiotensin Signaling in the Testes of Rats with TNBS-Induced Colitis"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5999-5140","authenticated-orcid":false,"given":"Micha\u0142","family":"Oczkowski","sequence":"first","affiliation":[{"name":"Department of Dietetics, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8069-6356","authenticated-orcid":false,"given":"Katarzyna","family":"Dziendzikowska","sequence":"additional","affiliation":[{"name":"Department of Dietetics, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Pasternak-Winiarska","sequence":"additional","affiliation":[{"name":"Department of Dietetics, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuba","family":"Jarmo\u0142owicz","sequence":"additional","affiliation":[{"name":"Department of Dietetics, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9529-3744","authenticated-orcid":false,"given":"Joanna","family":"Gromadzka-Ostrowska","sequence":"additional","affiliation":[{"name":"Department of Dietetics, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Huang, B., Wang, Z., Kong, Y., Jin, M., and Ma, L. (2023). Global, regional and national burden of male infertility in 204 countries and territories between 1990 and 2019: An analysis of global burden of disease study. BMC Public Health, 23.","DOI":"10.1186\/s12889-023-16793-3"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102298","DOI":"10.1016\/j.bpobgyn.2022.102298","article-title":"Environmental toxicants and male fertility","volume":"86","author":"Rodprasert","year":"2023","journal-title":"Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hussain, T., Metwally, E., Murtaza, G., Kalhoro, D.H., Chughtai, M.I., Tan, B., Omur, A.D., Tunio, S.A., Akbar, M.S., and Kalhoro, M.S. (2024). Redox mechanisms of environmental toxicants on male reproductive function. Front. Cell Dev. Biol., 12.","DOI":"10.3389\/fcell.2024.1333845"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1097\/MEG.0000000000001074","article-title":"Sexual and reproductive issues and inflammatory bowel disease: A neglected topic in men","volume":"30","author":"Allocca","year":"2018","journal-title":"Eur. J. Gastroenterol. Hepatol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chen, P., Zhou, G., Lin, J., Li, L., Zeng, Z., Chen, M., and Zhang, S. (2020). Serum Biomarkers for Inflammatory Bowel Disease. Front. Med., 7.","DOI":"10.3389\/fmed.2020.00123"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zou, H., Xu, N., Xu, H., Xing, X., Chen, Y., and Wu, S. (2024). Inflammatory cytokines may mediate the causal relationship between gut microbiota and male infertility: A bidirectional, mediating, multivariate Mendelian randomization study. Front. Endocrinol. (Lausanne), 15.","DOI":"10.3389\/fendo.2024.1368334"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1097\/MIB.0000000000001081","article-title":"Evaluation of the Quality of Semen and Sexual Function in Men with Inflammatory Bowel Disease","volume":"23","author":"Valer","year":"2017","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"155","DOI":"10.4103\/aja202353","article-title":"Inflammatory bowel disease (ulcerative colitis type) severity shows inverse correlation with semen parameters and testosterone levels","volume":"26","author":"Darmadi","year":"2023","journal-title":"Asian J. Androl."},{"key":"ref_9","first-page":"11100","article-title":"Semen and sex-steroid parameters among inflammatory bowel disease ulcerative colitis type according to rectal bleeding grade","volume":"95","author":"Darmadi","year":"2023","journal-title":"Arch. Ital. Urol. Androl."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.reprotox.2014.08.004","article-title":"Dextran sulfate sodium-induced ulcerative colitis leads to testicular toxicity in mice: Role of inflammation, oxidative stress and DNA damage","volume":"49","author":"Parmar","year":"2014","journal-title":"Reprod. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"111254","DOI":"10.1016\/j.mce.2021.111254","article-title":"Renin-angiotensin system and inflammation update","volume":"529","author":"Ramos","year":"2021","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1016\/j.juro.2014.05.093","article-title":"Beneficial effects of Renin-Angiotensin system blockers on testicular steroidogenesis","volume":"192","author":"Frantz","year":"2014","journal-title":"J. Urol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"154398","DOI":"10.1016\/j.phymed.2022.154398","article-title":"Botanicals in health and disease of the testis and male fertility: A scoping review","volume":"106","author":"Shepherd","year":"2022","journal-title":"Phytomedicine"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4545","DOI":"10.1080\/87559129.2022.2029477","article-title":"Bioactive Components and Health Functions of Oat","volume":"39","author":"Tang","year":"2023","journal-title":"Food Rev. Int."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s12253-017-0278-3","article-title":"High- and low-Molecular Weight oat Beta Glucan Reveals Antitumor Activity in Human Epithelial Lung Cancer","volume":"24","author":"Choromanska","year":"2018","journal-title":"Pathol. Oncol. Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gudej, S., Filip, R., Harasym, J., Wilczak, J., Dziendzikowska, K., Oczkowski, M., Ja\u0142osi\u0144ska, M., Juszczak, M., Lange, E., and Gromadzka-Ostrowska, J. (2021). Clinical outcomes after oat beta-glucans dietary treatment in gastritis patients. Nutrients, 13.","DOI":"10.3390\/nu13082791"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kopiasz, \u0141., Dziendzikowska, K., and Gromadzka-ostrowska, J. (2022). Colon Expression of Chemokines and Their Receptors Depending on the Stage of Colitis and Oat Beta-Glucan Dietary Intervention\u2014Crohn\u2019s Disease Model Study. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031406"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e13088","DOI":"10.1111\/and.13088","article-title":"The effects of dietary restriction and administration of \u03b2-glucan from Euglena gracilis on the sperm characteristics and reproductive organs of rats","volume":"50","author":"Alcay","year":"2018","journal-title":"Andrologia"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"53668","DOI":"10.1007\/s11356-021-14398-0","article-title":"Protective role of yeast beta-glucan on lead acetate-induced hepatic and reproductive toxicity in rats","volume":"28","author":"Seven","year":"2021","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Brenna, \u00d8., Furnes, M.W., Drozdov, I., van Beelen Granlund, A., Flatberg, A., Sandvik, A.K., Zwiggelaar, R.T., M\u00e5rvik, R., Nordrum, I.S., and Kidd, M. (2013). Relevance of TNBS-colitis in rats: A methodological study with endoscopic, histologic and Transcriptomic characterization and correlation to IBD. PLoS ONE, 8.","DOI":"10.1371\/annotation\/af20399c-993e-436c-ad06-e0d8a657a7b4"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"\u017by\u0142a, E., Dziendzikowska, K., Gajewska, M., Wilczak, J., Harasym, J., and Gromadzka-Ostrowska, J. (2019). Beneficial effects of oat beta-glucan dietary supplementation in colitis depend on its molecular weight. Molecules, 24.","DOI":"10.3390\/molecules24193591"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.jcs.2014.10.010","article-title":"Effect of size reduction by freeze-milling on processing properties of beta-glucan oat bran","volume":"61","author":"Harasym","year":"2015","journal-title":"J. Cereal Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1093\/ilar.43.4.207","article-title":"Sample size determination","volume":"43","author":"Dell","year":"2002","journal-title":"ILAR J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.taap.2016.10.013","article-title":"Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male rats","volume":"313","author":"Dziendzikowska","year":"2016","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Xu, M., Ling, F., Li, J., Chen, Y., Li, S., Cheng, Y., and Zhu, L. (2023). Oat beta-glucan reduces colitis by promoting autophagy flux in intestinal epithelial cells via EPHB6-TFEB axis. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1189229"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1475-2891-6-6","article-title":"Concentrated oat \u03b2-glucan, a fermentable fiber, lowers serum cholesterol in hypercholesterolemic adults in a randomized controlled trial","volume":"6","author":"Queenan","year":"2007","journal-title":"Nutr. J."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Crespo, I., San-Miguel, B., Mauriz, J.L., de Urbina, J.J.O., Almar, M., Tu\u00f1\u00f3n, M.J., and Gonz\u00e1lez-Gallego, J. (2017). Protective effect of protocatechuic acid on TNBS-induced colitis in mice is associated with modulation of the SphK\/S1P signaling pathway. Nutrients, 9.","DOI":"10.3390\/nu9030288"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ocampo, Y.C., Castro, J.P., P\u00e1jaro, I.B., Caro, D., Talero, E., Motilva, V., and Franco, L.A. (2024). Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0299687"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1203\/PDR.0b013e3181ffee6c","article-title":"Puberty is delayed in male mice with dextran sodium sulfate colitis out of proportion to changes in food intake, body weight, and serum levels of leptin","volume":"69","author":"Deboer","year":"2011","journal-title":"Pediatr. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Traish, A., Bolanos, J., Nair, S., Saad, F., and Morgentaler, A. (2018). Do Androgens Modulate the Pathophysiological Pathways of Inflammation? Appraising the Contemporary Evidence. J. Clin. Med., 7.","DOI":"10.3390\/jcm7120549"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"E1182","DOI":"10.1152\/ajpendo.00338.2019","article-title":"The gut microbiota is a major regulator of androgen metabolism in intestinal contents","volume":"317","author":"Landin","year":"2019","journal-title":"Am. J. Physiol.-Endocrinol. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Li, X., Yao, Z., Qian, J., Li, H., and Li, H. (2024). Lactate Protects Intestinal Epithelial Barrier Function from Dextran Sulfate Sodium-Induced Damage by GPR81 Signaling. Nutrients, 16.","DOI":"10.3390\/nu16050582"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1385\/ENDO:25:3:265","article-title":"Bacterial endotoxin lipopolysaccharide and reactive oxygen species inhibit Leydig cell steroidogenesis via perturbation of mitochondria","volume":"25","author":"Allen","year":"2004","journal-title":"Endocrine"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Shen, P., Ji, S., Li, X., Yang, Q., Xu, B., Wong, C.K.C., Wang, L., and Li, L. (2022). LPS-Induced Systemic Inflammation Caused mPOA-FSH\/LH Disturbance and Impaired Testicular Function. Front. Endocrinol. (Lausanne), 13.","DOI":"10.3389\/fendo.2022.886085"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1177\/0960327117703689","article-title":"6-Gingerol improves testicular function in mice model of chronic ulcerative colitis","volume":"37","author":"Farombi","year":"2018","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1016\/j.jinorgbio.2007.03.003","article-title":"Effects of vanadate on male rat reproductive tract histology, oxidative stress markers and androgenic enzyme activities","volume":"101","author":"Chandra","year":"2007","journal-title":"J. Inorg. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.freeradbiomed.2016.02.028","article-title":"The role of glutathione reductase and related enzymes on cellular redox homoeostasis network","volume":"95","author":"Couto","year":"2016","journal-title":"Free Radic. Biol. Med."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Kopiasz, \u0141., Dziendzikowska, K., Gajewska, M., Wilczak, J., Harasym, J., \u017by\u0142a, E., Kamola, D., Oczkowski, M., Kr\u00f3likowski, T., and Gromadzka-Ostrowska, J. (2020). Time-dependent indirect antioxidative effects of oat beta-glucans on peripheral blood parameters in the animal model of colon inflammation. Antioxidants, 9.","DOI":"10.3390\/antiox9050375"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Pei, J., Pan, X., Wei, G., and Hua, Y. (2023). Research progress of glutathione peroxidase family (GPX) in redoxidation. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1147414"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1039\/C4FO00638K","article-title":"The effect of low or high molecular weight oat beta-glucans on the inflammatory and oxidative stress status in the colon of rats with LPS-induced enteritis","volume":"6","author":"Wilczak","year":"2015","journal-title":"Food Funct."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kopiasz, \u0141., Dziendzikowska, K., Gajewska, M., Oczkowski, M., Majchrzak-Kuligowska, K., Kr\u00f3likowski, T., and Gromadzka-Ostrowska, J. (2021). Effects of dietary oat beta-glucans on colon apoptosis and autophagy through TLRs and dectin-1 signaling pathways\u2014Crohn\u2019s disease model study. Nutrients, 13.","DOI":"10.3390\/nu13020321"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.4049\/jimmunol.2100107","article-title":"\u03b2-Glucan Induces Distinct and Protective Innate Immune Memory in Differentiated Macrophages","volume":"207","author":"Stothers","year":"2021","journal-title":"J. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Gianzo, M., and Subir\u00e1n, N. (2020). Regulation of male fertility by the renin-angiotensin system. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21217943"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Shibata, T., Bhat, S.A., Cao, D.Y., Saito, S., Bernstein, E.A., Nishi, E., Medenilla, J.D., Wang, E.T., Chan, J.L., and Pisarska, M.D. (2024). Testicular ACE regulates sperm metabolism and fertilization through the transcription factor PPAR\u03b3. J. Biol. Chem., 300.","DOI":"10.1016\/j.jbc.2023.105486"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12967-022-03842-5","article-title":"Angiotensin-(1\u20137) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-\u03baB and MAPK pathways","volume":"21","author":"Chen","year":"2023","journal-title":"J. Transl. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5374897","DOI":"10.1155\/2017\/5374897","article-title":"Sulforaphane Prevents Angiotensin II-Induced Testicular Cell Death via Activation of NRF2","volume":"2017","author":"Wang","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"G774","DOI":"10.1152\/ajpgi.00053.2019","article-title":"Renin-angiotensin system promotes colonic inflammation by inducing TH17 activation via JAK2\/STAT pathway","volume":"316","author":"He","year":"2019","journal-title":"Am. J. Physiol.-Gastrointest. Liver Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.dci.2017.05.005","article-title":"Anti-inflammatory action of angiotensin 1\u20137 in experimental colitis may be mediated through modulation of serum cytokines\/chemokines and immune cell functions","volume":"74","author":"Khajah","year":"2017","journal-title":"Dev. Comp. Immunol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/15\/2546\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:29:28Z","timestamp":1760110168000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/15\/2546"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,3]]},"references-count":48,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["nu16152546"],"URL":"https:\/\/doi.org\/10.3390\/nu16152546","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,3]]}}}