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province","award":["2024JC-YBMS-764"],"award-info":[{"award-number":["2024JC-YBMS-764"]}]},{"name":"Open Fund for Key Laboratory of precision medicine for children\u2019s diseases in Shaanxi province","award":["KFJJ2023-01"],"award-info":[{"award-number":["KFJJ2023-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Puerarin is an isoflavone compound isolated from the roots of a leguminous plant, the wild kudzu. Various functional activities of this compound in multiple diseases have been reported. However, the effect and mechanism of puerarin in improving blood pressure remain non-elucidated. Purpose: The current study was designed to assess the preventive effects of puerarin on the onset and progression of hypertension and to verify the hypothesis that puerarin alleviates blood pressure by inhibiting the ROS\/TLR4\/NLRP3 inflammasome signaling pathway in the hypothalamic paraventricular nucleus (PVN) of salt-induced prehypertensive rats. Methods: Male Dahl salt-sensitive rats were fed low NaCl salt (3% in drinking water) for the control (NS) group or 8% (HS) to induce prehypertension. Each batch was divided into two group and treated by bilateral PVN microinjection with either artificial cerebrospinal fluid or puerarin through a micro-osmotic pump for 6 weeks. The mean arterial pressure (MAP) was recorded, and samples were collected and analyzed. Results: We concluded that puerarin significantly prevented the elevation of blood pressure and effectively alleviated the increase in heart rate caused by high salt. Norepinephrine (NE) in the plasma of salt-induced prehypertensive rats also decreased upon puerarin chronic infusion. Additionally, analysis of the PVN sample revealed that puerarin pretreatment decreased the positive cells and gene level of TLR4 (Toll-like receptor 4), NLRP3, Caspase-1 p10, NOX2, MyD88, NOX4, and proinflammatory cytokines in the PVN. Puerarin pretreatment also decreased NF-\u03baBp65 activity, inhibited oxidative stress, and alleviated inflammatory responses in the PVN. Conclusion: We conclude that puerarin alleviated blood pressure via inhibition of the ROS\/TLR4\/NLRP3 inflammasome signaling pathway in the PVN, suggesting the therapeutic potential of puerarin in the prevention of hypertension.<\/jats:p>","DOI":"10.3390\/nu16162580","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T08:07:59Z","timestamp":1722931679000},"page":"2580","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Puerarin Alleviates Blood Pressure via Inhibition of ROS\/TLR4\/NLRP3 Inflammasome Signaling Pathway in the Hypothalamic Paraventricular Nucleus of Salt-Induced Prehypertensive Rats"],"prefix":"10.3390","volume":"16","author":[{"given":"Hong-Li","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Institute of Cardiovascular Sciences, Translational Medicine Institute, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, Xi\u2019an Jiaotong University, Ministry of Education, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Institute of Cardiovascular Sciences, Translational Medicine Institute, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, Xi\u2019an Jiaotong University, Ministry of Education, Xi\u2019an 710061, China"},{"name":"Department of Pharmacology, Basic Medical College, Jiamusi University, Jiamusi 154007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Institute of Cardiovascular Sciences, Translational Medicine Institute, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, Xi\u2019an Jiaotong University, Ministry of Education, Xi\u2019an 710061, China"},{"name":"Department of Diagnosis, Shaanxi University of Chinese Medicine, Xi\u2019an 712046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen-Liang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi\u2019an Jiaotong University Health 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Yan","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Institute of Cardiovascular Sciences, Translational Medicine Institute, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, Xi\u2019an Jiaotong University, Ministry of Education, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi\u2019an Jiaotong University Health Science Center, Xi\u2019an 710061, China"},{"name":"Institute of Cardiovascular Sciences, Translational Medicine Institute, 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