{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T16:01:04Z","timestamp":1777737664352,"version":"3.51.4"},"reference-count":63,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Carbohydrate (CHO) metabolism is crucial for short-duration, high-intensity exercise performance, but the effects of variations in glycogen availability have not been investigated in field trials of trained athletes. This study was designed to test how 1500 m time trial (TT) performance is affected by the manipulation of pre-race glycogen reserves. Competitive middle-distance runners (n = 11 (4 females)) completed a 1500 m individually paced indoor TT after abundant (high, &gt;5 g\/kg\/d) or restricted (low, &lt;1.5 g\/kg\/d) dietary CHO intake for 2 days after a glycogen-depleting session. Stride pattern, heart rate (HR), capillary blood lactate, and glucose and plasma malondialdehyde (MDA) response were determined. The TT was slower in low vs. high condition by 4.5 (4.5) s (~2%; p &lt; 0.01), with a tendency toward shorter stride length. Blood lactate and glucose were lower before the TT in low vs. high condition (1.8 (0.5) vs. 2.2 (0.7) mmol\/L and 5.4 (0.7) vs. 5.9 (0.8) mmol\/L, p = 0.022 and 0.007, respectively), and peak lactate was higher in high vs. low condition (16.8 (3.1) vs. 14.5 (4.2) mmol\/L, p = 0.039). Plasma MDA was the same before the TT, and 15 min after the TT, it increased similarly by 15% in low (p = 0.032) and high (p = 0.005) conditions. The restriction of pre-test CHO intake impaired 1500 m TT performance and reduced baseline and peak blood lactate concentrations but not blood glucose or MDA response.<\/jats:p>","DOI":"10.3390\/nu16162763","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T10:11:28Z","timestamp":1724062288000},"page":"2763","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of Low vs. High Carbohydrate Intake after Glycogen-Depleting Workout on Subsequent 1500 m Run Performance in High-Level Runners"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1956-6616","authenticated-orcid":false,"given":"Tomas","family":"Venckunas","sequence":"first","affiliation":[{"name":"Institute of Sport Science and Innovations, Lithuanian Sports University, 44221 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-6050","authenticated-orcid":false,"given":"Petras","family":"Minderis","sequence":"additional","affiliation":[{"name":"Institute of Sport Science and Innovations, Lithuanian Sports University, 44221 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoras","family":"Silinskas","sequence":"additional","affiliation":[{"name":"Institute of Sport Science and Innovations, Lithuanian Sports University, 44221 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonsas","family":"Buliuolis","sequence":"additional","affiliation":[{"name":"Institute of Sport Science and Innovations, Lithuanian Sports University, 44221 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald J.","family":"Maughan","sequence":"additional","affiliation":[{"name":"School of Medicine, University of St Andrews, St Andrews KY16 9TF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigitas","family":"Kamandulis","sequence":"additional","affiliation":[{"name":"Institute of Sport Science and Innovations, Lithuanian Sports University, 44221 Kaunas, Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/j.1748-1716.1939.tb01320.x","article-title":"Arbeitsf\u00e4higkeit und Ern\u00e4hrung","volume":"81","author":"Christensen","year":"1939","journal-title":"Skand. Arch. Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1042\/bj0140290","article-title":"The Relative Value of Fat and Carbohydrate as Sources of Muscular Energy: With Appendices on the Correlation between Standard Metabolism and the Respiratory Quotient during Rest and Work","volume":"14","author":"Krogh","year":"1920","journal-title":"Biochem. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1001\/jama.1925.02670070028009","article-title":"Sugar content of the blood in runners following a marathon race: With especial reference to the prevention of hypoglycemia: Further observations","volume":"85","author":"Gordon","year":"1925","journal-title":"J. Am. Med. Assoc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/BF01746509","article-title":"Ueber die Bedeutung der verschiedenen N\u00e4hrstoffe als Erzeuger der Muskelkraft","volume":"83","author":"Zuntz","year":"1901","journal-title":"Arch. Physiol. Menschen Tiere"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/210309a0","article-title":"Muscle glycogen synthesis after exercise: An enhancing factor localized to the muscle cells in man","volume":"210","author":"Hultman","year":"1966","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1748-1716.1967.tb03719.x","article-title":"Muscle glycogen during prolonged severe exercise","volume":"71","author":"Hermansen","year":"1967","journal-title":"Acta Physiol. Scand."},{"key":"ref_7","first-page":"85","article-title":"Human muscle glycogen content and capacity for prolonged exercise after different diets","volume":"3","author":"Ahlborg","year":"1967","journal-title":"Forsvarmedicin"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1111\/j.1748-1716.1967.tb03720.x","article-title":"Diet, Muscle glycogen and physical performance","volume":"71","author":"Hermansen","year":"1967","journal-title":"Acta Physiol. Scand."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1113\/jphysiol.1974.sp010639","article-title":"Selective glycogen depletion pattern in human muscle fibres after exercise of varying intensity and at varying pedalling rates","volume":"241","author":"Gollnick","year":"1974","journal-title":"J. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Pernow, B., and Saltin, B. (1971). Muscle glycogen utilisation during work of different intensities. Muscle Metabolism during Exercise, Plenum Press.","DOI":"10.1007\/978-1-4613-4609-8"},{"key":"ref_11","first-page":"75","article-title":"Effect of metabolic changes on force generation in skeletal muscle during maximal exercise","volume":"82","author":"Hermansen","year":"1981","journal-title":"Ciba Found Symp."},{"key":"ref_12","first-page":"1","article-title":"Lactate, muscle glycogen and exercise performance in man","volume":"495","author":"Jacobs","year":"1981","journal-title":"Acta Physiol. Scand. Suppl."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1042\/cs0610331","article-title":"Effect of PH on muscle glycolysis during exercise","volume":"61","author":"Sutton","year":"1981","journal-title":"Clin. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/BF00418458","article-title":"The effects of diet on muscle pH and metabolism during high intensity exercise","volume":"57","author":"Greenhaff","year":"1988","journal-title":"Eur. J. Appl Physiol. Occup. Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1152\/jappl.1973.34.5.615","article-title":"Glycogen depletion pattern in human skeletal muscle fibers after heavy exercise","volume":"34","author":"Gollnick","year":"1973","journal-title":"J. Appl. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4405","DOI":"10.1113\/jphysiol.2013.251629","article-title":"Muscle glycogen stores and fatigue","volume":"591","author":"Westerblad","year":"2013","journal-title":"J. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1113\/jphysiol.2010.195982","article-title":"Role of glycogen availability in sarcoplasmic reticulum Ca2+ kinetics in human skeletal muscle","volume":"589","author":"Nielsen","year":"2011","journal-title":"J. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1249\/MSS.0000000000000132","article-title":"Muscle glycogen content modifies SR Ca2+ release rate in elite endurance athletes","volume":"46","author":"Gejl","year":"2014","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF00423397","article-title":"The effects of a glycogen-loading regimen on the capacity to perform anaerobic exercise","volume":"46","author":"Maughan","year":"1981","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1123\/ijsn.5.2.110","article-title":"A Carbohydrate loading regimen improves high intensity, short duration exercise performance","volume":"5","author":"Pizza","year":"1995","journal-title":"Int. J. Sport Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s004210050146","article-title":"Effect of muscle glycogen availability on maximal exercise performance","volume":"75","author":"Hargreaves","year":"1997","journal-title":"Eur. J. Appl. Physiol. Occup. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1113\/jphysiol.1992.sp019161","article-title":"Elevated muscle glycogen and anaerobic energy production during exhaustive exercise in man","volume":"451","author":"Bangsbo","year":"1992","journal-title":"J. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1136\/bjsm.28.3.171","article-title":"The influence of dietary carbohydrate and pre-exercise glucose consumption on supramaximal intermittent exercise performance","volume":"28","author":"Jenkins","year":"1994","journal-title":"Br. J. Sports Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1249\/00005768-199509000-00007","article-title":"No effect of glycogen level on glycogen metabolism during high intensity exercise","volume":"27","author":"Vandenberghe","year":"1995","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1080\/17461391.2022.2130097","article-title":"The effect of acute manipulation of carbohydrate availability on high intensity running performance, running economy, critical speed, and substrate metabolism in trained Male runners","volume":"23","author":"Ramonas","year":"2023","journal-title":"Eur. J. Sport Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1113\/JP285762","article-title":"Differential utilisation of subcellular skeletal muscle glycogen pools: A comparative analysis between 1 and 15 min of maximal exercise","volume":"602","author":"Schytz","year":"2024","journal-title":"J. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e374","DOI":"10.1016\/S2352-3026(17)30105-9","article-title":"Effects of erythropoietin on cycling performance of well trained cyclists: A double-blind, randomised, placebo-controlled trial","volume":"4","author":"Heuberger","year":"2017","journal-title":"Lancet Haematol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1123\/ijspp.2021-0451","article-title":"Defining Training and Performance Caliber: A Participant Classification Framework","volume":"17","author":"McKay","year":"2022","journal-title":"Int. J. Sports Physiol. Perform."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1123\/ijsnem.2019-0004","article-title":"Contemporary Nutrition Strategies to Optimize Performance in Distance Runners and Race Walkers","volume":"29","author":"Burke","year":"2019","journal-title":"Int. J. Sport Nutr. Exerc. Metab."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1111\/sms.13655","article-title":"Carbohydrates do not accelerate force recovery after glycogen-depleting followed by high-intensity exercise in humans","volume":"30","author":"Cheng","year":"2020","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"ref_31","first-page":"139","article-title":"Post-exercise muscle glycogen repletion in the extreme: Effect of food absence and active recovery","volume":"3","author":"Fournier","year":"2004","journal-title":"J. Sports Sci. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1080\/17461391.2016.1188992","article-title":"Perception of effort in Exercise Science: Definition, measurement and perspectives","volume":"16","author":"Pageaux","year":"2016","journal-title":"Eur. J. Sport Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1249\/MSS.0b013e31818cb278","article-title":"Progressive statistics for studies in sports medicine and exercise science","volume":"41","author":"Hopkins","year":"2009","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1007\/s40279-021-01475-0","article-title":"Muscle Glycogen Metabolism and High-Intensity Exercise Performance: A Narrative Review","volume":"51","author":"Spriet","year":"2021","journal-title":"Sports Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/BF00418466","article-title":"Diet-induced metabolic acidosis and the performance of high intensity exercise in man","volume":"57","author":"Greenhaff","year":"1988","journal-title":"Eur. J. Appl. Physiol. Occup. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s12970-018-0242-y","article-title":"ISSN exercise & sports nutrition review update: Research & recommendations","volume":"15","author":"Kerksick","year":"2018","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"S17","DOI":"10.1080\/02640414.2011.585473","article-title":"Carbohydrates for training and competition","volume":"29","author":"Burke","year":"2011","journal-title":"J. Sports Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Shiose, K., Takahashi, H., and Yamada, Y. (2022). Muscle Glycogen Assessment and Relationship with Body Hydration Status: A Narrative Review. Nutrients, 15.","DOI":"10.3390\/nu15010155"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1111\/sms.14354","article-title":"Lowered muscle glycogen reduces body mass with no effect on short-term exercise performance in men","volume":"33","author":"Schytz","year":"2023","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.1080\/02640414.2011.619204","article-title":"Dietary protein for athletes: From requirements to optimum adaptation","volume":"29","author":"Phillips","year":"2011","journal-title":"J. Sports Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1017\/S095442241700004X","article-title":"Dietary fibre in Europe: Current state of knowledge on definitions, sources, recommendations, intakes and relationships to health","volume":"30","author":"Stephen","year":"2017","journal-title":"Nutr. Res. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/BF00690901","article-title":"The effects of dietary manipulation on blood acid-base status and the performance of high intensity exercise","volume":"56","author":"Greenhaff","year":"1987","journal-title":"Eur. J. Appl Physiol Occup. Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1080\/026404197367272","article-title":"Diet composition and the performance of high-intensity exercise","volume":"15","author":"Maughan","year":"1997","journal-title":"J. Sports Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1152\/jappl.1971.31.2.203","article-title":"Diet, muscle glycogen, and endurance performance","volume":"31","author":"Karlsson","year":"1971","journal-title":"J. Appl. Physiol."},{"key":"ref_45","first-page":"S97","article-title":"Biochemistry of muscle fatigue","volume":"45","author":"Hultman","year":"1986","journal-title":"Biomed. Biochim. Acta"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1249\/01.MSS.0000058433.85789.66","article-title":"CNS Fatigue and Prolonged Exercise: Effect of Glucose Supplementation","volume":"35","author":"Nybo","year":"2003","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1249\/MSS.0000000000003002","article-title":"The Role of Muscle Glycogen Content and Localization in High-Intensity Exercise Performance: A Placebo-Controlled Trial","volume":"54","author":"Nielsen","year":"2022","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/sms.12599","article-title":"Muscle glycogen and cell function\u2014Location, location, location","volume":"25","author":"Nielsen","year":"2015","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3679","DOI":"10.1113\/jphysiol.2009.174862","article-title":"Distinct effects of subcellular glycogen localization on tetanic relaxation time and endurance in mechanically skinned rat skeletal muscle fibres","volume":"587","author":"Nielsen","year":"2009","journal-title":"J. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2871","DOI":"10.1113\/jphysiol.2010.204487","article-title":"Human skeletal muscle glycogen utilization in exhaustive exercise: Role of subcellular localization and fibre type","volume":"589","author":"Nielsen","year":"2011","journal-title":"J. Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1113\/JP273109","article-title":"Local depletion of glycogen with supramaximal exercise in human skeletal muscle fibres","volume":"595","author":"Gejl","year":"2017","journal-title":"J. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1113\/JP283225","article-title":"Fibre type- and localisation-specific muscle glycogen utilisation during repeated high-intensity intermittent exercise","volume":"600","author":"Andersen","year":"2022","journal-title":"J. Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1152\/jappl.1988.65.6.2601","article-title":"Carbohydrate balance in competitive runners during successive days of intense training","volume":"65","author":"Kirwan","year":"1988","journal-title":"J. Appl. Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"R1100","DOI":"10.1152\/ajpregu.00858.2005","article-title":"Manipulation of dietary carbohydrates after prolonged effort modifies muscle sarcoplasmic reticulum responses in exercising males","volume":"291","author":"Duhamel","year":"2006","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Venckunas, T., Brazaitis, M., Snieckus, A., Mickevicius, M., Eimantas, N., Subocius, A., Mickeviciene, D., Westerblad, H., and Kamandulis, S. (2022). Adding High-Intensity Interval Training to Classical Resistance Training Does Not Impede the Recovery from Inactivity-Induced Leg Muscle Weakness. Antioxidants, 12.","DOI":"10.3390\/antiox12010016"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1080\/02640410903473844","article-title":"Altered oxidative stress in overtrained athletes","volume":"28","author":"Tanskanen","year":"2010","journal-title":"J. Sports Sci."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Lugo, R., Avila-Nava, A., Pech-Aguilar, A.G., Medina-Vera, I., Guevara-Cruz, M., and Solis, A.L.G. (2022). Relationship between lipid accumulation product and oxidative biomarkers by gender in adults from Yucatan, Mexico. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-18705-8"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"R1896","DOI":"10.1152\/ajpregu.1994.266.6.R1896","article-title":"Plasma volume and ion regulation during exercise after low- and high-carbohydrate diets","volume":"266","author":"Lindinger","year":"1994","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1007\/s00421-012-2504-8","article-title":"Exercise with low glycogen increases PGC-1\u03b1 gene expression in human skeletal muscle","volume":"113","author":"Psilander","year":"2013","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1007\/s00421-021-04594-8","article-title":"Carbohydrate restriction following strenuous glycogen-depleting exercise does not potentiate the acute molecular response associated with mitochondrial biogenesis in human skeletal muscle","volume":"121","author":"Ramos","year":"2021","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1080\/02640414.2011.562232","article-title":"Effects of optimal pacing strategies for 400-, 800-, and 1500-m races on the VO2 response","volume":"29","author":"Hanon","year":"2011","journal-title":"J. Sports Sci."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"King, A.J., Etxebarria, N., Ross, M.L., Garvican-Lewis, L., Heikura, I.A., McKay, A.K.A., Tee, N., Forbes, S.F., Beard, N.A., and Saunders, P.U. (2022). Short-Term Very High Carbohydrate Diet and Gut-Training Have Minor Effects on Gastrointestinal Status and Performance in Highly Trained Endurance Athletes. Nutrients, 14.","DOI":"10.3390\/nu14091929"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1007\/s00421-024-05437-y","article-title":"Substrate utilization and durability during prolonged intermittent exercise in elite road cyclists","volume":"124","author":"Zachariassen","year":"2024","journal-title":"Eur. J. Appl. Physiol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/16\/2763\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:39:05Z","timestamp":1760110745000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/16\/2763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,19]]},"references-count":63,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["nu16162763"],"URL":"https:\/\/doi.org\/10.3390\/nu16162763","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,19]]}}}