{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:58:39Z","timestamp":1792159119398,"version":"4.4.0"},"reference-count":30,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T00:00:00Z","timestamp":1724544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Greece and the European Union (European Social FundESF)","award":["MIS 5048457"],"award-info":[{"award-number":["MIS 5048457"]}]},{"name":"Greece and the European Union (European Social FundESF)","award":["101080117"],"award-info":[{"award-number":["101080117"]}]},{"name":"European Union\u2019s Horizon Europe Research and Innovation Programme","award":["MIS 5048457"],"award-info":[{"award-number":["MIS 5048457"]}]},{"name":"European Union\u2019s Horizon Europe Research and Innovation Programme","award":["101080117"],"award-info":[{"award-number":["101080117"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Obesity is a critical public health issue, necessitating effective weight loss interventions. While various dietary regimens have been explored, individual responses to interventions often vary. This study involved a 3-month dietary intervention aiming at assessing the role of macronutrient composition and the potential role of genetic predisposition in weight loss among Greek adults. This randomized clinical trial followed the CONSORT principles, recruiting 202 participants overall; 94 received a hypocaloric, high-protein diet and 108 received a high-carbohydrate, hypocaloric diet. Genetic predispositions were assessed through 10 target variants known for their BMI associations. Participants\u2019 weight and BMI values were recorded at baseline and post-intervention (n = 202 at baseline, n = 84 post-intervention) and an imputation method was applied to account for the observed missing values. Participants experienced a statistically significant weight loss across all dietary regimens (p &lt; 0.001). Genetic analyses did not display statistically significant effects on weight loss. No significant differences in weight loss were observed between macronutrient groups, aligning with the POUNDS Lost and DIETFITS studies. This study underscores the importance of dietary interventions for weight loss and the potential contributions of genetic makeup. These findings contribute to obesity management within the Greek population and support the need for further research in personalized interventions.<\/jats:p>","DOI":"10.3390\/nu16172842","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T03:14:31Z","timestamp":1724642071000},"page":"2842","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Dietary Intervention in Adults with Overweight or Obesity Leads to Weight Loss Irrespective of Macronutrient Composition"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9665-0829","authenticated-orcid":false,"given":"Maria","family":"Kafyra","sequence":"first","affiliation":[{"name":"Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University of Athens, 17671 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-7491","authenticated-orcid":false,"given":"Ioanna Panagiota","family":"Kalafati","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University of Athens, 17671 Athens, Greece"},{"name":"Department of Nutrition and Dietetics, School of Physical Education, Sport Science and Dietetics, University of Thessaly, 42132 Trikala, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1828-537X","authenticated-orcid":false,"given":"Garyfallia","family":"Stefanou","sequence":"additional","affiliation":[{"name":"Biostatistics and Programming, ECONCARE, 11854 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1364-9488","authenticated-orcid":false,"given":"Georgia","family":"Kourlaba","sequence":"additional","affiliation":[{"name":"Department of Nursing, University of Peloponnese, 22100 Tripoli, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4199-0333","authenticated-orcid":false,"given":"Panagiotis","family":"Moulos","sequence":"additional","affiliation":[{"name":"Institute for Fundamental Biomedical Research, Biomedical Sciences Research Center \u2018Alexander Fleming\u2019, 16672 Vari, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0876-8167","authenticated-orcid":false,"given":"Iraklis","family":"Varlamis","sequence":"additional","affiliation":[{"name":"Department of Informatics and Telematics, School of Digital Technology, Harokopio University of Athens, 17778 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andriana C.","family":"Kaliora","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University of Athens, 17671 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7798-6221","authenticated-orcid":false,"given":"George V.","family":"Dedoussis","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University of Athens, 17671 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,25]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2024, August 14). Overweight and Obesity. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/obesity-and-overweight."},{"key":"ref_2","unstructured":"World Obesity Federation (2024, August 16). Report Card\u2014Greece. Available online: https:\/\/data.worldobesity.org\/country\/greece-80\/report-card.pdf."},{"key":"ref_3","unstructured":"World Health Organization (2024, July 17). The Challenge of Obesity. Available online: https:\/\/www.who.int\/europe\/news-room\/fact-sheets\/item\/the-challenge-of-obesity."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1038\/s41574-019-0176-8","article-title":"Obesity: Global epidemiology and pathogenesis","volume":"15","year":"2019","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e140065","DOI":"10.1172\/JCI140065","article-title":"Dietary interventions for obesity: Clinical and mechanistic findings","volume":"131","author":"Chao","year":"2021","journal-title":"J. Clin. Investig."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1159\/000528083","article-title":"European Association for the Study of Obesity Position Statement on Medical Nutrition Therapy for the Management of Overweight and Obesity in Adults Developed in Collaboration with the European Federation of the Associations of Dietitians","volume":"16","author":"Hassapidou","year":"2023","journal-title":"Obes. Facts"},{"key":"ref_7","unstructured":"The European Association for the Study of Obesity (2024, August 15). European Survey Finds over Three-Quarters of Adults with Obesity have Attempted to Lose Weight in the Past Year, But Most have Been Unsuccessful. Available online: https:\/\/easo.org\/european-survey-finds-over-three-quarters-of-adults-with-obesity-have-attempted-to-lose-weight-in-the-past-year-but-most-have-been-unsuccessful\/."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lu, X., Jin, Y., Li, D., Zhang, J., Han, J., and Li, Y. (2022). Multidisciplinary Progress in Obesity Research. Genes, 13.","DOI":"10.3390\/genes13101772"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Duarte, M.K.R.N., Leite-Lais, L., Agnez-Lima, L.F., Maciel, B.L.L., and Morais, A.H.A. (2024). Obesity and Nutrigenetics Testing: New Insights. Nutrients, 16.","DOI":"10.3390\/nu16050607"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41367-020-0014-4","article-title":"Nutrigenetics-personalized nutrition in obesity and cardiovascular diseases","volume":"10","author":"Barrea","year":"2020","journal-title":"Int. J. Obes. Suppl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1089\/pop.2021.0260","article-title":"The Impact of Expanding Telehealth-Delivered Dietary Interventions on Long-Term Cardiometabolic Health","volume":"25","author":"Li","year":"2022","journal-title":"Popul. Health Manag."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kafyra, M., Kalafati, I.P., Katsareli, E.A., Lambrinou, S., Varlamis, I., Kaliora, A.C., and Dedoussis, G.V. (2021). The iMPROVE Study; Design, Dietary Patterns, and Development of a Lifestyle Index in Overweight and Obese Greek Adults. Nutrients, 13.","DOI":"10.3390\/nu13103495"},{"key":"ref_13","unstructured":"ThermoFisher Scientific (2024, August 24). iPrep\u2122 PureLink\u2122 gDNA Blood Kit. Available online: https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/iprep_bloodgDNA_man.pdf."},{"key":"ref_14","unstructured":"Axiom\u2122 Precision Medicine Diversity Array Plus Kit, 96-Format (2024, July 17). Thermoficher Scientific. Available online: https:\/\/www.thermofisher.com\/order\/catalog\/product\/951961#\/951961."},{"key":"ref_15","unstructured":"(2024, July 17). Axiom\u2122 Precision Medicine Research Array. Available online: https:\/\/www.thermofisher.com\/order\/catalog\/product\/902981."},{"key":"ref_16","unstructured":"(2024, July 17). Infinium Global Screening Array-24 Kit. Available online: https:\/\/emea.illumina.com\/products\/by-type\/microarray-kits\/infinium-global-screening.html."},{"key":"ref_17","unstructured":"(2024, July 17). IMPUTE2. Available online: https:\/\/mathgen.stats.ox.ac.uk\/impute\/impute_v2.html."},{"key":"ref_18","unstructured":"(2024, July 17). GIANT Consortium. Available online: https:\/\/portals.broadinstitute.org\/collaboration\/giant\/index.php\/GIANT_consortium."},{"key":"ref_19","unstructured":"IBM Support (2024, August 24). SPSS Statistics 23.0 Now Available for Download. Available online: https:\/\/www.ibm.com\/support\/pages\/spss-statistics-230-now-available-download."},{"key":"ref_20","unstructured":"The R Project for Statistical Computing (2024, July 17). Last Modified in 2020. Available online: https:\/\/www.rproject.org\/."},{"key":"ref_21","unstructured":"StataCorp (2021). Statistical Software, Stata: Release 17, StataCorp LLC."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Schulz, K.F., Altman, D.G., Moher, D., and CONSORT Group (2010). CONSORT 2010 Statement: Updated guidelines for reporting parallel group randomised trials. BMC Med., 8.","DOI":"10.1186\/1741-7015-8-18"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1111\/dom.14155","article-title":"Genetic variation in lean body mass, changes of appetite and weight loss in response to diet interventions: The POUNDS Lost trial","volume":"22","author":"Li","year":"2020","journal-title":"Diabetes Obes. Metab."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1056\/NEJMoa0804748","article-title":"Comparison of weight-loss diets with different compositions of fat, protein, and carbohydrates","volume":"360","author":"Sacks","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1001\/jama.2018.0245","article-title":"Effect of Low-Fat vs Low-Carbohydrate Diet on 12-Month Weight Loss in Overweight Adults and the Association with Genotype Pattern or Insulin Secretion: The DIETFITS Randomized Clinical Trial","volume":"319","author":"Gardner","year":"2018","journal-title":"JAMA"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1002\/oby.21451","article-title":"A randomized trial of high-dairy-protein, variable-carbohydrate diets and exercise on body composition in adults with obesity","volume":"24","author":"Parr","year":"2016","journal-title":"Obesity"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"S\u00f8rensen, T.I., Boutin, P., Taylor, M.A., Larsen, L.H., Verdich, C., Petersen, L., Holst, C., Echwald, S.M., Dina, C., and Toubro, S. (2006). Genetic polymorphisms and weight loss in obesity: A randomised trial of hypo-energetic high- versus low-fat diets. PLoS Clin. Trials, 1.","DOI":"10.1371\/journal.pctr.0010023"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1159\/000345951","article-title":"Clinical correlates of weight loss and attrition during a 10-week dietary intervention study: Results from the NUGENOB project","volume":"5","author":"Holst","year":"2012","journal-title":"Obes. Facts"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"m696","DOI":"10.1136\/bmj.m696","article-title":"Comparison of dietary macronutrient patterns of 14 popular named dietary programmes for weight and cardiovascular risk factor reduction in adults: Systematic review and network meta-analysis of randomised trials","volume":"369","author":"Ge","year":"2020","journal-title":"BMJ"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s00592-021-01787-7","article-title":"Nutrigenetic variants and response to diet\/lifestyle intervention in obese subjects: A pilot study","volume":"59","author":"Franzago","year":"2022","journal-title":"Acta Diabetol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/17\/2842\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:42:31Z","timestamp":1760110951000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/17\/2842"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,25]]},"references-count":30,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["nu16172842"],"URL":"https:\/\/doi.org\/10.3390\/nu16172842","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,25]]}}}