{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:32:17Z","timestamp":1787841137515,"version":"build-2784847793"},"reference-count":34,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Lodz","award":["503\/1-107-03\/503-11-001"],"award-info":[{"award-number":["503\/1-107-03\/503-11-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>We present results on the potential protective antioxidant properties of indole-3-butyric acid. Indole-3-butyric acid is an indole derivative defined as an auxin and widely known as a plant growth regulator. It naturally occurs in Arabidopsis thaliana, which is applied as a model plant in genetic studies. Oxidative damage to membrane lipids (lipid peroxidation; LPO) in porcine thyroid homogenates was induced by Fenton reaction substrates (Fe2+ + H2O2). Iron (Fe2+) was used in very high concentrations of 1200, 600, 300, 150, 75, 37.5, 18.75, 9.375, 4.687, and 2.343 \u00b5M. Indole-3-butyric acid (10.0, 5.0, 2.5, 1.25, and 0.625 mM) was applied to check whether it prevents the above process. The LPO level, expressed as malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration, was measured spectrophotometrically. Expectedly, Fenton reaction substrates, in a Fe2+ concentration-dependent manner, increased LPO level, with the lowest effective concentration of iron being 9.375 \u00b5M. In the case of almost all concentrations of indole-3-butyric acid, this auxin has exhibited very promising antioxidant protection, with the most effective concentrations being 10.0 and 5.0 mM; however, as low concentrations of indole-3-butyric acid at 1.25 mM was still effective. Indole-3-butyric acid used alone did not change the basal level of LPO, which is a favourable effect. To summarise, indole-3-butyric acid has protective antioxidant properties against experimentally induced oxidative damage to membrane lipids in the thyroid, and this is for the first time documented in the literature. This compound can be considered a natural protective agent present in plants, which can serve as a dietary nutrient.<\/jats:p>","DOI":"10.3390\/nu16173010","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T03:22:46Z","timestamp":1725592966000},"page":"3010","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Indole-3-Butyric Acid, a Natural Auxin, Protects against Fenton Reaction-Induced Oxidative Damage in Porcine Thyroid"],"prefix":"10.3390","volume":"16","author":[{"given":"Anna K.","family":"Skoczy\u0144ska","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 281\/289 Rzgowska St., 93-338 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8901-7081","authenticated-orcid":false,"given":"Aleksandra K.","family":"G\u0142adysz","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 281\/289 Rzgowska St., 93-338 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7547-6228","authenticated-orcid":false,"given":"Jan","family":"St\u0119pniak","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 281\/289 Rzgowska St., 93-338 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3045-5669","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Karbownik-Lewi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 281\/289 Rzgowska St., 93-338 Lodz, Poland"},{"name":"Polish Mother\u2019s Memorial Hospital\u2014Research Institute, 281\/289 Rzgowska St., 93-338 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7343","DOI":"10.1039\/D1CS00265A","article-title":"Autoxidation vs. antioxidants\u2014The fight for forever","volume":"50","author":"Helberg","year":"2021","journal-title":"Chem. 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