{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T16:33:08Z","timestamp":1774542788811,"version":"3.50.1"},"reference-count":79,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Efeom Nutrition S.A.","award":["01\/04\u201321"],"award-info":[{"award-number":["01\/04\u201321"]}]},{"name":"Efeom Nutrition S.A.","award":["88887.703169\/2022\u201300"],"award-info":[{"award-number":["88887.703169\/2022\u201300"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["01\/04\u201321"],"award-info":[{"award-number":["01\/04\u201321"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.703169\/2022\u201300"],"award-info":[{"award-number":["88887.703169\/2022\u201300"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Stress, unhealthy lifestyle, and sleep disturbance worsen cognitive function in mood disorders, prompting a rise in the development of integrative health approaches. The recent investigations in the gut\u2013brain axis field highlight the strong interplay among microbiota, inflammation, and mental health. Thus, this study aimed to investigate a new nutraceutical formulation comprising prebiotics, minerals, and silymarin\u2019s impact on microbiota, inflammation, mood, and sleep quality. The study evaluated the LL1 + silymarin capsule supplementation over 180 days in overweight adults. We analyzed the fecal gut microbiota using partial 16S rRNA sequences, measured cytokine expression via CBA, collected anthropometric data, quality of life, and sleep questionnaire responses, and obtained plasma samples for metabolic and hormonal analysis at baseline (T0) and 180 days (T180) post-supplementation. Our findings revealed significant reshaping in gut microbiota composition at the phylum, genus, and species levels, especially in the butyrate-producer bacteria post-supplementation. These changes in gut microbiota were linked to enhancements in sleep quality, mood perception, cytokine expression, and anthropometric measures which microbiota-derived short-chain fatty acids might enhance. The supplementation tested in this study seems to be able to improve microbiota composition, reflecting anthropometrics and inflammation, as well as sleep quality and mood improvement.<\/jats:p>","DOI":"10.3390\/nu16183049","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T03:16:51Z","timestamp":1725938211000},"page":"3049","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Nutraceutical Capsules LL1 and Silymarin Supplementation Act on Mood and Sleep Quality Perception by Microbiota\u2013Gut\u2013Brain Axis: A Pilot Clinical Study"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3109-3745","authenticated-orcid":false,"given":"Aline Boveto","family":"Santamarina","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Produtos e Derivados Naturais, Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica-26 (LIM-26), Departamento de Cirurgia, Faculdade de Medicina de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, SP, Brazil"},{"name":"Pesquisa e Desenvolvimento Efeom Nutri\u00e7\u00e3o S\/A, S\u00e3o Paulo 03317-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Nehmi Filho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos e Derivados Naturais, Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica-26 (LIM-26), Departamento de Cirurgia, Faculdade de Medicina de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, SP, Brazil"},{"name":"Pesquisa e Desenvolvimento Efeom Nutri\u00e7\u00e3o S\/A, S\u00e3o Paulo 03317-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9457-4587","authenticated-orcid":false,"given":"J\u00e9ssica Alves de","family":"Freitas","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos e Derivados Naturais, Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica-26 (LIM-26), Departamento de Cirurgia, Faculdade de Medicina de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, SP, Brazil"},{"name":"Pesquisa e Desenvolvimento Efeom Nutri\u00e7\u00e3o S\/A, S\u00e3o Paulo 03317-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6034-920X","authenticated-orcid":false,"given":"Lucas Augusto Moys\u00e9s","family":"Franco","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia M\u00e9dica (LIM-46), Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, Instituto de Medicina Tropical de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joyce Vanessa","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica em Protozoologia, Bacteriologia e Resist\u00eancia Antimicrobiana (LIM-49), Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, Instituto de Medicina Tropical de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta Cristina","family":"Martins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia M\u00e9dica (LIM-46), Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, Instituto de Medicina Tropical de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2366-921X","authenticated-orcid":false,"given":"Jos\u00e9 Ant\u00f4nio Orellana","family":"Turri","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Economia da Sa\u00fade, Departamento de Ginecologia e Obstetr\u00edcia, Faculdade de Medicina, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna Fernanda Rio Branco da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos e Derivados Naturais, Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica-26 (LIM-26), Departamento de Cirurgia, Faculdade de Medicina de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, SP, Brazil"},{"name":"Laborat\u00f3rio Interdisciplinar em Fisiologia e Exerc\u00edcio, Universidade Federal de S\u00e3o Paulo (UNIFESP), Santos 11015-020, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arianne Fagotti","family":"Gusm\u00e3o","sequence":"additional","affiliation":[{"name":"International Research Center, A.C. 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