{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T13:34:31Z","timestamp":1791466471684,"version":"4.3.4"},"reference-count":46,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T00:00:00Z","timestamp":1726185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fuji Foundation for Protein Research and the Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JP22K17782"],"award-info":[{"award-number":["JP22K17782"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Sarcopenic obesity, which is associated with a poorer prognosis than that of sarcopenia alone, may be positively affected by soy isoflavones, known inhibitors of muscle atrophy. Herein, we hypothesize that these compounds may prevent sarcopenic obesity by upregulating the gut metabolites with anti-inflammatory effects. Methods: To explore the effects of soy isoflavones on sarcopenic obesity and its mechanisms, we employed both in vivo and in vitro experiments. Mice were fed a high-fat, high-sucrose diet with or without soy isoflavone supplementation. Additionally, the mouse C2C12 myotube cells were treated with palmitic acid and daidzein in vitro. Results: The isoflavone considerably reduced muscle atrophy and the expression of the muscle atrophy genes in the treated group compared to the control group (Fbxo32, p = 0.0012; Trim63, p &lt; 0.0001; Foxo1, p &lt; 0.0001; Tnfa, p = 0.1343). Elevated levels of daidzein were found in the muscles and feces of the experimental group compared to the control group (feces, p = 0.0122; muscle, p = 0.0020). The real-time PCR results demonstrated that the daidzein decreased the expression of the palmitate-induced inflammation and muscle atrophy genes in the C2C12 myotube cells (Tnfa, p = 0.0201; Il6, p = 0.0008; Fbxo32, p &lt; 0.0001; Hdac4, p = 0.0002; Trim63, p = 0.0114; Foxo1, p &lt; 0.0001). Additionally, it reduced the palmitate-induced protein expression related to the muscle atrophy in the C2C12 myotube cells (Foxo1, p = 0.0078; MuRF1, p = 0.0119). Conclusions: The daidzein suppressed inflammatory cytokine- and muscle atrophy-related gene expression in the C2C12 myotubes, thereby inhibiting muscle atrophy.<\/jats:p>","DOI":"10.3390\/nu16183084","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T05:31:44Z","timestamp":1726205504000},"page":"3084","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Daidzein Inhibits Muscle Atrophy by Suppressing Inflammatory Cytokine- and Muscle Atrophy-Related Gene Expression"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9603-1802","authenticated-orcid":false,"given":"Chihiro","family":"Munekawa","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7269-1697","authenticated-orcid":false,"given":"Takuro","family":"Okamura","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saori","family":"Majima","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Budau","family":"River","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2984-5094","authenticated-orcid":false,"given":"Sayaka","family":"Kawai","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3209-1790","authenticated-orcid":false,"given":"Hanako","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuko","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1707-970X","authenticated-orcid":false,"given":"Hiroshi","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Senmaru","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1031-4380","authenticated-orcid":false,"given":"Emi","family":"Ushigome","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8651-4445","authenticated-orcid":false,"given":"Masahide","family":"Hamaguchi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0903-1797","authenticated-orcid":false,"given":"Michiaki","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/ageing\/afq034","article-title":"Sarcopenia: European Consensus on Definition and Diagnosis: Report of the European Working Group on Sarcopenia in Older People","volume":"39","author":"Baeyens","year":"2010","journal-title":"Age Ageing"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"502","DOI":"10.3177\/jnsv.66.502","article-title":"Health Promotion Effects of Soy Isoflavones","volume":"66","author":"Nakai","year":"2020","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s13098-023-01022-z","article-title":"Causal Relationship between Insulin Resistance and Sarcopenia","volume":"15","author":"Liu","year":"2023","journal-title":"Diabetol. Metab. Syndr."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ali, A.M., and Kunugi, H. (2020). Foods Apitherapy for Age-Related Skeletal Muscle Dysfunction (Sarcopenia): A Review on the Effects of Royal Jelly, Propolis, and Bee Pollen. Foods, 9.","DOI":"10.3390\/foods9101362"},{"key":"ref_5","first-page":"k1651","article-title":"Associations of Grip Strength with Cardiovascular, Respiratory, and Cancer Outcomes and All Cause Mortality: Prospective Cohort Study of Half a Million UK Biobank Participants","volume":"361","author":"Welsh","year":"2018","journal-title":"BMJ"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S0140-6736(19)32008-2","article-title":"Modifiable Risk Factors, Cardiovascular Disease, and Mortality in 155 722 Individuals from 21 High-Income, Middle-Income, and Low-Income Countries (PURE): A Prospective Cohort Study","volume":"395","author":"Yusuf","year":"2020","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1111\/j.1749-6632.2000.tb06498.x","article-title":"Body Composition in Healthy Aging","volume":"904","author":"Baumgartner","year":"2000","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1038\/ejcn.2012.43","article-title":"Adverse Effects of Coexistence of Sarcopenia and Metabolic Syndrome in Japanese Women","volume":"66","author":"Sanada","year":"2012","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jamda.2013.11.025","article-title":"Sarcopenia in Asia: Consensus Report of the Asian Working Group for Sarcopenia","volume":"15","author":"Chen","year":"2014","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e13534","DOI":"10.1111\/obr.13534","article-title":"Deciphering the \u201cObesity Paradox\u201d in the Elderly: A Systematic Review and Meta-Analysis of Sarcopenic Obesity","volume":"24","author":"Liu","year":"2023","journal-title":"Obes. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"495","DOI":"10.4014\/jmb.2311.11034","article-title":"Gromwell (Lithospermum Erythrorhizon) Attenuates High-Fat-Induced Skeletal Muscle Wasting by Increasing Protein Synthesis and Mitochondrial Biogenesis","volume":"34","author":"Kim","year":"2024","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/ijo.2009.130","article-title":"Prevalence of Sarcopenia and Sarcopenic Obesity in Korean Adults: The Korean Sarcopenic Obesity Study","volume":"33","author":"Kim","year":"2009","journal-title":"Int. J. Obes."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4422","DOI":"10.1016\/j.clnu.2021.01.005","article-title":"Prevalence of Sarcopenic Obesity and Sarcopenic Overweight in the General Population: The Lifelines Cohort Study","volume":"40","author":"Wagenaar","year":"2021","journal-title":"Clin. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4633","DOI":"10.1016\/j.clnu.2021.06.009","article-title":"Global Prevalence of Sarcopenic Obesity in Older Adults: A Systematic Review and Meta-Analysis","volume":"40","author":"Gao","year":"2021","journal-title":"Clin. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"E243604","DOI":"10.1001\/jamanetworkopen.2024.3604","article-title":"Sarcopenia and Sarcopenic Obesity and Mortality among Older People","volume":"7","author":"Benz","year":"2024","journal-title":"JAMA Netw. Open"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s00394-017-1593-x","article-title":"The Influence of Isoflavone for Denervation-Induced Muscle Atrophy","volume":"58","author":"Tabata","year":"2019","journal-title":"Eur. J. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1093\/advances\/nmy065","article-title":"The Impact of Dairy Protein Intake on Muscle Mass, Muscle Strength, and Physical Performance in Middle-Aged to Older Adults with or without Existing Sarcopenia: A Systematic Review and Meta-Analysis","volume":"10","author":"Hanach","year":"2019","journal-title":"Adv. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1093\/ajcn\/nqy192","article-title":"Effects of Protein Supplementation on Lean Body Mass, Muscle Strength, and Physical Performance in Nonfrail Community-Dwelling Older Adults: A Systematic Review and Meta-Analysis","volume":"108","author":"Nuijten","year":"2018","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ganapathy, A., and Nieves, J.W. (2020). Nutrition and Sarcopenia-What Do We Know?. Nutrients, 12.","DOI":"10.3390\/nu12061755"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wu, H., Quan, J., Wang, X., Gu, Y., Zhang, S., Meng, G., Zhang, Q., Liu, L., Wang, X., and Sun, S. (2023). Soy Food Consumption Is Inversely Associated with Handgrip Strength: Results from the TCLSIH Cohort Study. Nutrients, 15.","DOI":"10.3390\/nu15020391"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Okamura, T., Hashimoto, Y., Majima, S., Senmaru, T., Ushigome, E., Nakanishi, N., Asano, M., Yamazaki, M., Takakuwa, H., and Hamaguchi, M. (2021). Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.669672"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3028","DOI":"10.1002\/jcsm.13076","article-title":"Brazilian Green Propolis Improves Gut Microbiota Dysbiosis and Protects against Sarcopenic Obesity","volume":"13","author":"Okamura","year":"2022","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Okamura, T., Hashimoto, Y., Mori, J., Yamaguchi, M., Majima, S., Senmaru, T., Ushigome, E., Nakanishi, N., Asano, M., and Yamazaki, M. (2021). ILC2s Improve Glucose Metabolism Through the Control of Saturated Fatty Acid Absorption Within Visceral Fat. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.669629"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sakuma, K., Hamada, K., Yamaguchi, A., and Aoi, W. (2023). Current Nutritional and Pharmacological Approaches for Attenuating Sarcopenia. Cells, 12.","DOI":"10.3390\/cells12192422"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Chadha, R., Bhalla, Y., Jain, A., Chadha, K., and Karan, M. (2020). The Role of Isoflavones in Type 2 Diabetes Prevention and Treatment\u2014A Narrative Review. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22010218"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jsbmb.2015.09.002","article-title":"The Effect of Glucuronidation on Isoflavone Induced Estrogen Receptor (ER)\u03b1 and ER\u03b2 Mediated Coregulator Interactions","volume":"154","author":"Beekmann","year":"2015","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3177\/jnsv.59.317","article-title":"Isoflavones Derived from Soy Beans Prevent MuRF1-Mediated Muscle Atrophy in C2C12 Myotubes through SIRT1 Activation","volume":"59","author":"Hirasaka","year":"2013","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4363","DOI":"10.1002\/ptr.7132","article-title":"Daidzein Alleviates Cisplatin-Induced Muscle Atrophy by Regulating Glut4\/AMPK\/FoxO Pathway","volume":"35","author":"Zhang","year":"2021","journal-title":"Phytother. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jnutbio.2017.07.017","article-title":"Daidzein Down-Regulates Ubiquitin-Specific Protease 19 Expression through Estrogen Receptor \u03b2 and Increases Skeletal Muscle Mass in Young Female Mice","volume":"49","author":"Ogawa","year":"2017","journal-title":"J. Nutr. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s11130-022-00984-1","article-title":"Hypolipidemic Effects of Soy Protein and Isoflavones in the Prevention of Non-Alcoholic Fatty Liver Disease- A Review","volume":"77","author":"Xiao","year":"2022","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1016\/j.lfs.2005.03.021","article-title":"High-Fat Diet Induces Increased Tissue Expression of TNF-\u03b1","volume":"77","author":"Borst","year":"2005","journal-title":"Life Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Rasool, S., Geetha, T., Broderick, T.L., and Babu, J.R. (2018). High Fat With High Sucrose Diet Leads to Obesity and Induces Myodegeneration. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.01054"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1007\/s11306-022-01944-1","article-title":"Isoflavone Consumption Reduces Inflammation through Modulation of Phenylalanine and Lipid Metabolism","volume":"18","author":"Shrode","year":"2022","journal-title":"Metabolomics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3177\/jnsv.62.178","article-title":"Dietary Supplementation with Isofl Avones Prevents Muscle Wasting in Tumor-Bearing Mice","volume":"62","author":"Hirasaka","year":"2016","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Singh, S., Grewal, S., Sharma, N., Behl, T., Gupta, S., Anwer, M.K., Vargas-De-La-Cruz, C., Mohan, S., Bungau, S.G., and Bumbu, A. (2023). Unveiling the Pharmacological and Nanotechnological Facets of Daidzein: Present State-of-the-Art and Future Perspectives. Molecules, 28.","DOI":"10.3390\/molecules28041765"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1093\/ajcn\/76.2.447","article-title":"Evidence for Lack of Absorption of Soy Isoflavone Glycosides in Humans, Supporting the Crucial Role of Intestinal Metabolism for Bioavailability","volume":"76","author":"Setchell","year":"2002","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"V\u00e1zquez, L., Bel\u00e9n Fl\u00f3rez, A., Guadamuro, L., and Mayo, B. (2017). Effect of Soy Isoflavones on Growth of Representative Bacterial Species from the Human Gut. Nutrients, 9.","DOI":"10.3390\/nu9070727"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.abb.2014.06.007","article-title":"Absorption, Distribution, Metabolism, and Excretion of Isoflavonoids after Soy Intake","volume":"559","author":"Franke","year":"2014","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8710","DOI":"10.18632\/aging.103197","article-title":"Soy Isoflavones Improve the Oxidative Stress Induced Hypothalamic Inflammation and Apoptosis in High Fat Diet-Induced Obese Male Mice through PGC1-Alpha Pathway","volume":"12","author":"Pang","year":"2020","journal-title":"Aging"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Kitamura, K., Erlangga, J.S., Tsukamoto, S., Sakamoto, Y., Mabashi-Asazuma, H., and Iida, K. (2020). Daidzein Promotes the Expression of Oxidative Phosphorylation- and Fatty Acid Oxidation-Related Genes via an Estrogen-Related Receptor \u03b1 Pathway to Decrease Lipid Accumulation in Muscle Cells. J. Nutr. Biochem., 77.","DOI":"10.1016\/j.jnutbio.2019.108315"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1096\/fj.06-7537com","article-title":"TNF-Related Weak Inducer of Apoptosis (TWEAK) Is a Potent Skeletal Muscle-Wasting Cytokine","volume":"21","author":"Dogra","year":"2007","journal-title":"FASEB J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e13784","DOI":"10.1111\/obr.13784","article-title":"Bibliometric Analysis of Research on Sarcopenic Obesity: A Review of Scientific Literature","volume":"25","year":"2024","journal-title":"Obes. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1017\/S0007114522001787","article-title":"Effects of Whey and Soy Protein Supplementation on Inflammatory Cytokines in Older Adults: A Systematic Review and Meta-Analysis","volume":"129","author":"Prokopidis","year":"2023","journal-title":"Br. J. Nutr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"165","DOI":"10.5891\/jafps.29.165","article-title":"Effect of Variety of Soybean Seeds and Processing of Soy Milk on the Isoflavone Content of Tofu","volume":"29","author":"Endo","year":"2003","journal-title":"Food Preserv. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"322","DOI":"10.3136\/nskkk.42.322","article-title":"Changes of isoflavones at various stages of fermentation in defatted soybeans","volume":"42","author":"Ikeda","year":"1995","journal-title":"Nippon Shokuhin Kagaku Kogaku Kaishi"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"620","DOI":"10.6013\/jbrewsocjapan1988.94.620","article-title":"Functional Properties of Fermented Products-Isoflavones in Miso","volume":"94","author":"Kiyosawa","year":"1999","journal-title":"J. Brew. Soc. Jpn."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/18\/3084\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:55:27Z","timestamp":1760111727000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/18\/3084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,13]]},"references-count":46,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["nu16183084"],"URL":"https:\/\/doi.org\/10.3390\/nu16183084","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,13]]}}}