{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:16:57Z","timestamp":1777490217539,"version":"3.51.4"},"reference-count":32,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: The definition of alcohol hangovers refers to a combination of mental and physical side effects that occur after drinking. One of the ways that hangovers can be ameliorated is by promoting the rapid and effective elimination of acetaldehyde to alleviate the discomfort it causes. This study aimed to investigate the effects of GSH (yeast extract containing 50 mg of glutathione) on the hangover-relieving effect. Methods: A randomized double-blind placebo-controlled crossover clinical trial was conducted with 40 participants who reported experiencing hangover symptoms. Participants consumed alcohol at a rate of 0.78 g per kg body weight with 40% whiskey, adjusted according to their weight. Alcohol and acetaldehyde concentrations in serum were analyzed at 0, 0.25, 1, 2, 4, 6, and 15 h after alcohol consumption. Results: In the GSH group, the serum alcohol concentration decreased, although this change was not statistically significant. The serum acetaldehyde concentration was significantly lower in the GSH group in comparison to the placebo group (at 0.25, 1, 4, and 6 h (p &lt; 0.01) and at 0.5, 2, and 15 h (p &lt; 0.001) after alcohol consumption). However, there was no significant difference between the two groups on questionnaires such as the Acute Hangover Scale and the Alcohol Hangover Severity Scale. Conclusions: Overall, we consider the discovery that GSH lowered acetaldehyde concentration, a crucial factor in alcohol metabolism, to be more considerable. Therefore, GSH administration effectively reduces acetaldehyde levels in serum. This result suggests that this effect may contribute to the relief of hangover symptoms.<\/jats:p>","DOI":"10.3390\/nu16193262","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T03:44:13Z","timestamp":1727408653000},"page":"3262","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of GSH on Alcohol Metabolism and Hangover Improvement in Humans: A Randomized Double-Blind Placebo-Controlled Crossover Clinical Trial"],"prefix":"10.3390","volume":"16","author":[{"given":"Gunju","family":"Song","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam-si 13488, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyein","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam-si 13488, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyoung","family":"Park","sequence":"additional","affiliation":[{"name":"Food R&D, Samyang Corp., Seongnam-si 13488, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5736-8813","authenticated-orcid":false,"given":"Soonok","family":"Sa","sequence":"additional","affiliation":[{"name":"Food R&D, Samyang Corp., Seongnam-si 13488, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wookyung","family":"Chung","sequence":"additional","affiliation":[{"name":"Food R&D, Samyang Corp., Seongnam-si 13488, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boo Yong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam-si 13488, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Khamis, A.A., Salleh, S.Z., Ab Karim, M.S., Mohd Rom, N.A., Janasekaran, S., Idris, A., and Abd Rashid, R.B. (2022). Alcohol Consumption Patterns: A Systematic Review of Demographic and Sociocultural Influencing Factors. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19138103"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.3906\/sag-2002-237","article-title":"Alcohol Consumption and Alcohol Policy","volume":"50","author":"Ilhan","year":"2020","journal-title":"Turk. J. Med. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Terpstra, C., Verster, J.C., Scholey, A., and Benson, S. (2022). Associations between Mental Resilience, Mood, Coping, Personality, and Hangover Severity. J. Clin. Med., 11.","DOI":"10.3390\/jcm11082240"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Verster, J.C., Scholey, A., van de Loo, A.J.A.E., Benson, S., and Stock, A.-K. (2020). Updating the Definition of the Alcohol Hangover. J. Clin. Med., 9.","DOI":"10.3390\/jcm9030823"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s13679-024-00560-5","article-title":"Alcohol Drinking Impacts on Adiposity and Steatotic Liver Disease: Concurrent Effects on Metabolic Pathways and Cardiovascular Risks","volume":"13","author":"Landecho","year":"2024","journal-title":"Curr. Obes. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1111\/add.15202","article-title":"Socio-Demographic Differences in Alcohol-Related Work Impairment","volume":"116","author":"Moan","year":"2021","journal-title":"Addiction"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wang, F., Li, Y., Zhang, Y.-J., Zhou, Y., Li, S., and Li, H.-B. (2016). Natural Products for the Prevention and Treatment of Hangover and Alcohol Use Disorder. Molecules, 21.","DOI":"10.3390\/molecules21010064"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e2600","DOI":"10.1002\/hup.2600","article-title":"Interventions for Treatment and\/or Prevention of Alcohol Hangover: Systematic Review","volume":"32","author":"Jayawardena","year":"2017","journal-title":"Human Psychopharmacol. Clin. Exp."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hyun, J., Han, J., Lee, C., Yoon, M., and Jung, Y. (2021). Pathophysiological Aspects of Alcohol Metabolism in the Liver. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115717"},{"key":"ref_10","first-page":"265","article-title":"The metabolism of alcohol","volume":"74","author":"Paquot","year":"2019","journal-title":"Rev. Med. Liege"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1080\/03602532.2019.1679169","article-title":"Ethanol and Its Metabolites: Update on Toxicity, Benefits, and Focus on Immunomodulatory Effects","volume":"51","author":"Lagente","year":"2019","journal-title":"Drug Metab. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Jiang, Y., Zhang, T., Kusumanchi, P., Han, S., Yang, Z., and Liangpunsakul, S. (2020). Alcohol Metabolizing Enzymes, Microsomal Ethanol Oxidizing System, Cytochrome P450 2E1, Catalase, and Aldehyde Dehydrogenase in Alcohol-Associated Liver Disease. Biomedicines, 8.","DOI":"10.3390\/biomedicines8030050"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/978-3-319-98788-0_3","article-title":"Glutathione and Transsulfuration in Alcohol-Associated Tissue Injury and Carcinogenesis","volume":"1032","author":"Chen","year":"2018","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1097\/01.ALC.0000089958.65095.84","article-title":"Binding of Acetaldehyde to a Glutathione Metabolite: Mass Spectrometric Characterization of an Acetaldehyde-Cysteinylglycine Conjugate","volume":"27","author":"Anni","year":"2003","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Tao, Z., Yuan, H., Liu, M., Liu, Q., Zhang, S., Liu, H., Jiang, Y., Huang, D., and Wang, T. (2023). Yeast Extract: Characteristics, Production, Applications and Future Perspectives. J. Microbiol. Biotechnol., 33.","DOI":"10.4014\/jmb.2207.07057"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.mam.2008.08.009","article-title":"Structure, Function, and Post-Translational Regulation of the Catalytic and Modifier Subunits of Glutamate Cysteine Ligase","volume":"30","author":"Franklin","year":"2009","journal-title":"Mol. Asp. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5028181","DOI":"10.1155\/2019\/5028181","article-title":"Glutathione \u201cRedox Homeostasis\u201d and Its Relation to Cardiovascular Disease","volume":"2019","author":"Bajic","year":"2019","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"282","DOI":"10.3746\/pnf.2022.27.3.282","article-title":"Evaluation of Phenolic Content Diversity along with Antioxidant\/Pro-Oxidant, Glutathione Transferase Inhibition, and Cytotoxic Potential of Selected Commonly Used Plants","volume":"27","author":"Guneidy","year":"2022","journal-title":"Prev. Nutr. Food Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s00253-012-4147-4","article-title":"Novel Physiological Roles for Glutathione in Sequestering Acetaldehyde to Confer Acetaldehyde Tolerance in Saccharomyces Cerevisiae","volume":"97","author":"Matsufuji","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.freeradbiomed.2012.11.010","article-title":"Aldehyde Dehydrogenases in Cellular Responses to Oxidative\/Electrophilicstress","volume":"56","author":"Singh","year":"2013","journal-title":"Free Radic. Biol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1007\/BF01966681","article-title":"Conjugation of Acetaldehyde with Cysteinylglycine, the First Metabolite in Glutathione Breakdown by Gamma-Glutamyltranspeptidase","volume":"17","author":"Kera","year":"1985","journal-title":"Agents Actions"},{"key":"ref_22","unstructured":"The Ministry of Food and Drug Safety in Korea (2023). Guidelines for Human Clinical Trials of Hangover Cure Claims, The Ministry of Food and Drug Safety in Korea."},{"key":"ref_23","unstructured":"The Ministry of Food and Drug Safety in Korea (2024). Food Additives Code, The Ministry of Food and Drug Safety in Korea."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"102066","DOI":"10.1016\/j.arr.2023.102066","article-title":"Glutathione and Glutathione-Dependent Enzymes: From Biochemistry to Gerontology and Successful Aging","volume":"92","author":"Lapenna","year":"2023","journal-title":"Ageing Res. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/s41467-022-28242-7","article-title":"Endogenous Formaldehyde Scavenges Cellular Glutathione Resulting in Redox Disruption and Cytotoxicity","volume":"13","author":"Umansky","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_26","first-page":"610","article-title":"Therapeutic Potential of Glutathione","volume":"112","author":"Exner","year":"2000","journal-title":"Wien. Klin. Wochenschr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.1749-6632.2002.tb04690.x","article-title":"Glutathione in Defense and Signaling","volume":"973","author":"Dickinson","year":"2002","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"178","DOI":"10.4161\/oxim.3.3.12288","article-title":"Acetaldehyde Adducts in Alcoholic Liver Disease","volume":"3","author":"Setshedi","year":"2010","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_29","first-page":"285","article-title":"Dangerous Byproducts of Alcohol Breakdown\u2014Focus on Adducts","volume":"27","author":"Tuma","year":"2003","journal-title":"Alcohol. Res. Health"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1021\/acs.chemrestox.9b00373","article-title":"Sequestration and Elimination of Toxic Aldehydes","volume":"33","author":"Nagasawa","year":"2020","journal-title":"Chem. Res. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1016\/0006-2952(85)90249-7","article-title":"Effects of Gamma-Glutamyltranspeptidase Inhibitor and Reduced Glutathione on Renal Acetaldehyde Levels in Rats","volume":"34","author":"Kera","year":"1985","journal-title":"Biochem. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Jeong, I.-K., Han, A., Jun, J.E., Hwang, Y.-C., Ahn, K.J., Chung, H.Y., Kang, B.S., and Choung, S.-Y. (2024). A Compound Containing Aldehyde Dehydrogenase Relieves the Effects of Alcohol Consumption and Hangover Symptoms in Healthy Men: An Open-Labeled Comparative Study. Pharmaceuticals, 17.","DOI":"10.3390\/ph17081087"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/19\/3262\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:04:24Z","timestamp":1760112264000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/19\/3262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,26]]},"references-count":32,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["nu16193262"],"URL":"https:\/\/doi.org\/10.3390\/nu16193262","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,26]]}}}