{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:30:33Z","timestamp":1760146233513,"version":"build-2065373602"},"reference-count":71,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Evonik Operations GmbH, Animal Nutrition Services Department, Hanau-Wolfgang, Germany","award":["039216001","039218001","069028"],"award-info":[{"award-number":["039216001","039218001","069028"]}]},{"name":"Elsa Neumann stipend of the State of Berlin","award":["039216001","039218001","069028"],"award-info":[{"award-number":["039216001","039218001","069028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Methionine (Met) is a popular nutritional supplement in humans and animals. It is routinely supplemented to pigs as L-Met, DL-Met, or DL-2-hydroxy-4-(methylthio) butanoic acid (DL-HMTBA). Methods: We investigated the effect of these Met supplements on jejunal amino acid (AA) transport in male castrated Pi\u00e9train \u00d7 Danbred pigs, also including a non-supplemented group. The mucosal-to-serosal flux of ten [14C]-labeled AAs (L-glutamine, glycine, L-leucine, L-lysine, L-Met, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine) was investigated at two concentrations (50 \u00b5M and 5 mM). Inhibition of apical uptake by mucosal L-Met was also measured for these AAs. The intestinal expression of apical AA transporters, angiotensin-converting enzyme II and inflammation-related genes were compared with those of a previous study. Results: Except for tryptophan and lysine at 5 mM, all AA fluxes were Na+-dependent (p \u2264 0.05), and the uptake of most AAs, except glycine and lysine, was inhibited by L-Met (p &lt; 0.001). A correlation network existed between Na+-dependent fluxes of most AAs (except tryptophan and partly glycine). We observed the upregulation of B0AT1 (SLC6A19) (p &lt; 0.001), the downregulation of ATB0,+ (SLC6A14) (p &lt; 0.001) and a lower expression of CASP1, IL1\u03b2, IL8, TGF\u03b2 and TNF\u03b1 in the present vs. the previous study (p &lt; 0.001). Conclusions: The correlating AAs likely share the same Na+-dependent transporter(s). A varying effect of the Met supplement type on AA transport in the two studies might be related to a different level of supplementation or a different inflammatory status of the small intestine.<\/jats:p>","DOI":"10.3390\/nu16193418","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T12:22:48Z","timestamp":1728476568000},"page":"3418","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine"],"prefix":"10.3390","volume":"16","author":[{"given":"Isabel I.","family":"Schermuly","sequence":"first","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, K\u00f6nigsweg 56, 14163 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-0787","authenticated-orcid":false,"given":"Stella","family":"Romanet","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, K\u00f6nigsweg 56, 14163 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1410-0902","authenticated-orcid":false,"given":"Amlan K.","family":"Patra","sequence":"additional","affiliation":[{"name":"American Institute for Goat Research, Langston University, Langston, OK 73050, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Mastrototaro","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, K\u00f6nigsweg 56, 14163 Berlin, Germany"},{"name":"Institute for Clinical Diabetology, German Diabetes Center, Leibniz Institute for Diabetes Research at Heinrich-Heine-University, Auf\u2019m Hennekamp 65, 40225 D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2483-1506","authenticated-orcid":false,"given":"Andreas","family":"Lemme","sequence":"additional","affiliation":[{"name":"Animal Nutrition Services, Evonik Operations GmbH, Rodenbacher Chausee 4, 63457 Hanau-Wolfgang, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0292-1482","authenticated-orcid":false,"given":"Robert","family":"Pieper","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Freie Universit\u00e4t Berlin, K\u00f6nigin-Luise-Stra\u00dfe 49, 14195 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Zentek","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Freie Universit\u00e4t Berlin, K\u00f6nigin-Luise-Stra\u00dfe 49, 14195 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5080-1007","authenticated-orcid":false,"given":"J\u00f6rg R.","family":"Aschenbach","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, K\u00f6nigsweg 56, 14163 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s00726-008-0152-4","article-title":"Amino acids and gut function","volume":"37","author":"Wang","year":"2009","journal-title":"Amino Acids"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1002\/iub.1571","article-title":"Gastrointestinal methionine shuttle: Priority handling of precious goods","volume":"68","author":"Mastrototaro","year":"2016","journal-title":"IUBMB Life"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1096\/fasebj.10.4.8647346","article-title":"S-Adenosylmethionine and methylation","volume":"10","author":"Chiang","year":"1996","journal-title":"FASEB J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1111\/j.1753-4887.2011.00466.x","article-title":"Sulfur amino acid metabolism and requirements","volume":"70","author":"Ball","year":"2012","journal-title":"Nutr. 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