{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T17:53:24Z","timestamp":1785866004468,"version":"3.56.0"},"reference-count":49,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T00:00:00Z","timestamp":1731715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of California, Davis, the University of California Cancer Research Coordinating Committee","award":["UC-CRCC # C23CR5560"],"award-info":[{"award-number":["UC-CRCC # C23CR5560"]}]},{"name":"NIFA-USDA","award":["CA-D-NTR-2397-H"],"award-info":[{"award-number":["CA-D-NTR-2397-H"]}]},{"name":"National Cancer Institute","award":["NCI P30CA093373"],"award-info":[{"award-number":["NCI P30CA093373"]}]},{"name":"EMBO Postdoctoral Fellowship","award":["ALTF 498-2023"],"award-info":[{"award-number":["ALTF 498-2023"]}]},{"DOI":"10.13039\/100016953","name":"UC Davis Comprehensive Cancer Center","doi-asserted-by":"crossref","award":["P30CA093373"],"award-info":[{"award-number":["P30CA093373"]}],"id":[{"id":"10.13039\/100016953","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: High-fat diets (HFDs) have been associated with an increased risk of pancreatic cancer. In contrast, ketogenic diets (KDs) have been shown to display anti-tumor characteristics. The objective of this work was to evaluate the efficacy of a KD on late-stage pancreatic carcinogenesis in a genetically modified mouse model of pancreatic cancer [LSL-KrasG12D\/+; Ptf1-Cre (KC) mice], as well as its liver safety, and to compare it to that of an HFD. Methods: Six-month-old female and male KC mice were randomly allocated to either a control diet (CD) (%kcal: 20% fat, 15% protein, 65% carbohydrates), an HFD (%kcal: 40% fat, 15% protein, 45% carbohydrate) or a KD (%kcal: 84% fat, 15% protein, 1% carbohydrate) and fed these diets for 6 months. Results: HFD-fed, but not KD-fed, mice showed a 15% increase in body weight, plus elevated serum insulin (2.4-fold increase) and leptin (2.9-fold increase) levels, compared to CD-fed mice. At the pancreas level, no differences in pancreatic cancer incidence rates were observed among the diet groups. Regarding the liver safety profile, the HFD-fed mice had higher serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), when compared to the CD and KD groups. In addition, upon histologic examination, an HFD, but not a KD, showed a ~2-fold increase in both macro- and microsteatosis, as well as 35% and 32% higher levels of TLR4 and NF-\u03baB activation, respectively, compared to CD-fed mice. Conclusions: In summary, although a KD intervention alone did not prevent pancreatic carcinogenesis, our data suggests that a KD modulates insulin signaling and hepatic lipid metabolism, highlighting its beneficial effects on healthspan and liver function when compared to an HFD.<\/jats:p>","DOI":"10.3390\/nu16223919","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T06:06:54Z","timestamp":1731996414000},"page":"3919","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Impact of a Ketogenic Diet on Late-Stage Pancreatic Carcinogenesis in Mice: Efficacy and Safety Studies"],"prefix":"10.3390","volume":"16","author":[{"given":"Natalia","family":"Cortez","sequence":"first","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"},{"name":"Department of Clinical and Biological Sciences, 10125 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9245-1970","authenticated-orcid":false,"given":"Tarek","family":"Bacha","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Ead","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Rodriguez Lanzi","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Lacroix","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anais","family":"Franceschetti","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7080-2744","authenticated-orcid":false,"given":"Brian","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Matsukuma","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, USA"},{"name":"Davis Comprehensive Cancer Center, University of California, Sacramento, CA 95817, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6460-7165","authenticated-orcid":false,"given":"Gerardo","family":"Mackenzie","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"},{"name":"Davis Comprehensive Cancer Center, University of California, Sacramento, CA 95817, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1158\/0008-5472.CAN-14-0155","article-title":"Projecting cancer incidence and deaths to 2030: The unexpected burden of thyroid, liver, and pancreas cancers in the United States","volume":"74","author":"Rahib","year":"2014","journal-title":"Cancer Res."},{"key":"ref_2","first-page":"12","article-title":"Cancer statistics, 2024","volume":"74","author":"Siegel","year":"2024","journal-title":"CA A Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1038\/nrgastro.2017.12","article-title":"Acinar cell plasticity and development of pancreatic ductal adenocarcinoma","volume":"14","author":"Storz","year":"2017","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_4","first-page":"358","article-title":"Pancreatic adenocarcinoma pathology: Changing \u201clandscape\u201d","volume":"6","author":"Brosens","year":"2015","journal-title":"J. Gastrointest. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.soc.2015.11.003","article-title":"Molecular and Genetic Basis of Pancreatic Carcinogenesis: Which Concepts May be Clinically Relevant?","volume":"25","author":"Bernard","year":"2016","journal-title":"Surg. Oncol. Clin. N. Am."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"680536","DOI":"10.5402\/2013\/680536","article-title":"The Association between Obesity and Cancer Risk: A Meta-Analysis of Observational Studies from 1985 to 2011","volume":"2013","author":"Dobbins","year":"2013","journal-title":"ISRN Prev. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1053\/j.gastro.2013.08.018","article-title":"A high-fat diet activates oncogenic Kras and COX2 to induce development of pancreatic ductal adenocarcinoma in mice","volume":"145","author":"Philip","year":"2013","journal-title":"Gastroenterology"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chang, H.H., Moro, A., Takakura, K., Su, H.Y., Mo, A., Nakanishi, M., Waldron, R.T., French, S.W., Dawson, D.W., and Hines, O.J. (2017). Incidence of pancreatic cancer is dramatically increased by a high fat, high calorie diet in KrasG12D mice. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0184455"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhu, L., Ji, J., Ma, J., Wang, D., Liu, M., Du, J.X., Chen, R., Hou, W., Abbruzzese, J.L., and Logsdon, C.D. (2022). Differential Effects of Dietary Macronutrients on the Development of Oncogenic KRAS-Mediated Pancreatic Ductal Adenocarcinoma. Cancers, 14.","DOI":"10.3390\/cancers14112723"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ead, A.S., Wirkus, J., Matsukuma, K., and Mackenzie, G.G. (2024). A high-fat diet induces changes in mesenteric adipose tissue accelerating early-stage pancreatic carcinogenesis in mice. J. Nutr. Biochem., 131.","DOI":"10.1016\/j.jnutbio.2024.109690"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/S1474-4422(08)70092-9","article-title":"The ketogenic diet for the treatment of childhood epilepsy: A randomised controlled trial","volume":"7","author":"Neal","year":"2008","journal-title":"Lancet Neurol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/978-3-319-42118-6_11","article-title":"Ketogenic Diet and Cancer-a Perspective","volume":"207","author":"Smyl","year":"2016","journal-title":"Recent Results Cancer Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cortez, N.E., and Mackenzie, G.G. (2021). Ketogenic Diets in Pancreatic Cancer and Associated Cachexia: Cellular Mechanisms and Clinical Perspectives. Nutrients, 13.","DOI":"10.3390\/nu13093202"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Abdelwahab, M.G., Fenton, K.E., Preul, M.C., Rho, J.M., Lynch, A., Stafford, P., and Scheck, A.C. (2012). The ketogenic diet is an effective adjuvant to radiation therapy for the treatment of malignant glioma. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036197"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1158\/2767-9764.CRC-22-0256","article-title":"A ketogenic diet in combination with gemcitabine increases survival in pancreatic cancer KPC mice","volume":"2","author":"Cortez","year":"2022","journal-title":"Cancer Res. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.ccr.2005.04.023","article-title":"Trp53R172H and KrasG12D cooperate to promote chromosomal instability and widely metastatic pancreatic ductal adenocarcinoma in mice","volume":"7","author":"Hingorani","year":"2005","journal-title":"Cancer Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/S1535-6108(03)00309-X","article-title":"Preinvasive and invasive ductal pancreatic cancer and its early detection in the mouse","volume":"4","author":"Hingorani","year":"2003","journal-title":"Cancer Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1158\/0008-5472.CAN-05-2168","article-title":"Pathology of genetically engineered mouse models of pancreatic exocrine cancer: Consensus report and recommendations","volume":"66","author":"Hruban","year":"2006","journal-title":"Cancer Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Liang, W., Menke, A.L., Driessen, A., Koek, G.H., Lindeman, J.H., Stoop, R., Havekes, L.M., Kleemann, R., and van den Hoek, A.M. (2014). Establishment of a general NAFLD scoring system for rodent models and comparison to human liver pathology. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115922"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.neo.2021.12.004","article-title":"Phospho-Aspirin (MDC-22) inhibits pancreatic cancer growth in patient-derived tumor xenografts and KPC mice by targeting EGFR: Enhanced efficacy in combination with irinotecan","volume":"24","author":"Wei","year":"2022","journal-title":"Neoplasia"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"479","DOI":"10.3892\/ol.2023.14067","article-title":"Hepatic safety profile of pancreatic cancer-bearing mice fed a ketogenic diet in combination with gemcitabine","volume":"26","author":"Cortez","year":"2023","journal-title":"Oncol. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1016\/j.cmet.2023.10.003","article-title":"Hyperinsulinemia acts via acinar insulin receptors to initiate pancreatic cancer by increasing digestive enzyme production and inflammation","volume":"35","author":"Zhang","year":"2023","journal-title":"Cell Metab."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1194\/jlr.M400261-JLR200","article-title":"Regulation of SREBP-1 expression and transcriptional action on HKII and FAS genes during fasting and refeeding in rat tissues","volume":"46","author":"Gosmain","year":"2005","journal-title":"J. Lipid Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.cmet.2011.03.009","article-title":"AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin-resistant mice","volume":"13","author":"Li","year":"2011","journal-title":"Cell Metab."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1210\/er.2018-00064","article-title":"Molecular Actions of PPARalpha in Lipid Metabolism and Inflammation","volume":"39","author":"Bougarne","year":"2018","journal-title":"Endocr. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cmet.2011.06.002","article-title":"Akt stimulates hepatic SREBP1c and lipogenesis through parallel mTORC1-dependent and independent pathways","volume":"14","author":"Yecies","year":"2011","journal-title":"Cell Metab."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/jgh.12019","article-title":"Toll-like receptors in alcoholic liver disease, non-alcoholic steatohepatitis and carcinogenesis","volume":"28","author":"Roh","year":"2013","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1158\/1940-6207.CAPR-13-0065","article-title":"High-fat, high-calorie diet promotes early pancreatic neoplasia in the conditional KrasG12D mouse model","volume":"6","author":"Dawson","year":"2013","journal-title":"Cancer Prev. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"G699","DOI":"10.1152\/ajpgi.00123.2018","article-title":"Dietary fat stimulates pancreatic cancer growth and promotes fibrosis of the tumor microenvironment through the cholecystokinin receptor","volume":"315","author":"Nadella","year":"2018","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1007\/s11605-010-1349-x","article-title":"Insulin, leptin, and tumoral adipocytes promote murine pancreatic cancer growth","volume":"14","author":"White","year":"2010","journal-title":"J. Gastrointest. Surg. Off. J. Soc. Surg. Aliment. Tract"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Mendonsa, A.M., Chalfant, M.C., Gorden, L.D., and VanSaun, M.N. (2015). Modulation of the leptin receptor mediates tumor growth and migration of pancreatic cancer cells. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0126686"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7347","DOI":"10.1073\/pnas.1922344117","article-title":"Effect of a ketogenic diet on hepatic steatosis and hepatic mitochondrial metabolism in nonalcoholic fatty liver disease","volume":"117","author":"Luukkonen","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1002\/cncr.20672","article-title":"Cytokines in pancreatic carcinoma: Correlation with phenotypic characteristics and prognosis","volume":"101","author":"Ebrahimi","year":"2004","journal-title":"Cancer"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1007\/s001250100021","article-title":"A choice of death--the signal-transduction of immune-mediated beta-cell apoptosis","volume":"44","author":"Eizirik","year":"2001","journal-title":"Diabetologia"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5977","DOI":"10.2147\/JIR.S381807","article-title":"The Pre-Ablation Circulating Tumor-Associated Inflammatory Index Predicts the Prognosis of Patients with Liver Metastasis from Pancreatic Cancer","volume":"15","author":"Li","year":"2022","journal-title":"J. Inflamm. Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Bae, H.R., Shin, S.K., Han, Y., Yoo, J.H., Kim, S., Young, H.A., and Kwon, E.Y. (2023). D-Allulose Ameliorates Dysregulated Macrophage Function and Mitochondrial NADH Homeostasis, Mitigating Obesity-Induced Insulin Resistance. Nutrients, 15.","DOI":"10.3390\/nu15194218"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.18632\/oncotarget.1426","article-title":"Chemokines in tumor progression and metastasis","volume":"4","author":"Sarvaiya","year":"2013","journal-title":"Oncotarget"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1136\/jim-2017-000524","article-title":"Influence of dietary macronutrients on liver fat accumulation and metabolism","volume":"65","author":"Parry","year":"2017","journal-title":"J. Investig. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.cmet.2018.01.005","article-title":"An Integrated Understanding of the Rapid Metabolic Benefits of a Carbohydrate-Restricted Diet on Hepatic Steatosis in Humans","volume":"27","author":"Mardinoglu","year":"2018","journal-title":"Cell Metab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"26649","DOI":"10.1074\/jbc.C500213200","article-title":"(D)-beta-Hydroxybutyrate inhibits adipocyte lipolysis via the nicotinic acid receptor PUMA-G","volume":"280","author":"Taggart","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1002\/hep.26592","article-title":"NLRP3 inflammasome activation results in hepatocyte pyroptosis, liver inflammation, and fibrosis in mice","volume":"59","author":"Wree","year":"2014","journal-title":"Hepatology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3642035","DOI":"10.1155\/2020\/3642035","article-title":"Long-Term Ketogenic Diet Induces Metabolic Acidosis, Anemia, and Oxidative Stress in Healthy Wistar Rats","volume":"2020","author":"Arsyad","year":"2020","journal-title":"J. Nutr. Metab."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1002\/hep4.1304","article-title":"Development of Hepatic Steatosis After Chemotherapy for Non-Hodgkin Lymphoma","volume":"3","author":"Alao","year":"2019","journal-title":"Hepatol. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Longo, R., Peri, C., Cricri, D., Coppi, L., Caruso, D., Mitro, N., De Fabiani, E., and Crestani, M. (2019). Ketogenic Diet: A New Light Shining on Old but Gold Biochemistry. Nutrients, 11.","DOI":"10.3390\/nu11102497"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Pinto, A., Bonucci, A., Maggi, E., Corsi, M., and Businaro, R. (2018). Anti-Oxidant and Anti-Inflammatory Activity of Ketogenic Diet: New Perspectives for Neuroprotection in Alzheimer\u2019s Disease. Antioxidants, 7.","DOI":"10.3390\/antiox7050063"},{"key":"ref_46","first-page":"3506213","article-title":"The Anti-Inflammatory Effect of Preventive Intervention with Ketogenic Diet Mediated by the Histone Acetylation of mGluR5 Promotor Region in Rat Parkinson\u2019s Disease Model: A Dual-Tracer PET Study","volume":"2022","author":"Zhu","year":"2022","journal-title":"Park. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ciaffi, J., Mitselman, D., Mancarella, L., Brusi, V., Lisi, L., Ruscitti, P., Cipriani, P., Meliconi, R., Giacomelli, R., and Borghi, C. (2021). The Effect of Ketogenic Diet on Inflammatory Arthritis and Cardiovascular Health in Rheumatic Conditions: A Mini Review. Front. Med., 8.","DOI":"10.3389\/fmed.2021.792846"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Dynka, D., Rodzen, L., Rodzen, M., Lojko, D., Kraszewski, S., Ibrahim, A., Hussey, M., Deptula, A., Grzywacz, Z., and Ternianov, A. (2024). Beneficial Effects of the Ketogenic Diet on Nonalcoholic Fatty Liver Disease (NAFLD\/MAFLD). J. Clin. Med., 13.","DOI":"10.3390\/jcm13164857"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e13024","DOI":"10.1111\/obr.13024","article-title":"Beneficial effects of the ketogenic diet on nonalcoholic fatty liver disease: A comprehensive review of the literature","volume":"21","author":"Watanabe","year":"2020","journal-title":"Obes. Rev."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/22\/3919\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T15:24:08Z","timestamp":1774884248000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/16\/22\/3919"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,16]]},"references-count":49,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["nu16223919"],"URL":"https:\/\/doi.org\/10.3390\/nu16223919","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,16]]}}}