{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:13:53Z","timestamp":1760058833938,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"high-level talents starting the project of JIT","award":["jit-b-202036","31972598","BY2022605","BK20240222"],"award-info":[{"award-number":["jit-b-202036","31972598","BY2022605","BK20240222"]}]},{"name":"National Natural Science Foundation of China","award":["jit-b-202036","31972598","BY2022605","BK20240222"],"award-info":[{"award-number":["jit-b-202036","31972598","BY2022605","BK20240222"]}]},{"name":"Industry-University-Research Project of Jiangsu Province","award":["jit-b-202036","31972598","BY2022605","BK20240222"],"award-info":[{"award-number":["jit-b-202036","31972598","BY2022605","BK20240222"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["jit-b-202036","31972598","BY2022605","BK20240222"],"award-info":[{"award-number":["jit-b-202036","31972598","BY2022605","BK20240222"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>In animal production, nanoparticulate zinc oxide exhibits synergistic antibacterial efficacy coupled with growth-promoting effects, positioning itself as a novel antibiotic alternative with enhanced biosafety profiles. However, its dose-dependent toxicity poses challenges. Objective: The experimental design sought to quantify the protective effects of dietary rutin against zinc-overload-induced damage. Methods: A zinc-overload murine model was established by giving high-dose ZnO nanoparticles (HZn, 5000 mg\/kg\/day) for 21 days. Mice were then fed rutin at doses of 300, 600, or 1200 mg\/kg. Body weight, relative organ indexes, zinc concentrations, serum enzyme activities, and tissue-level indicators of apoptosis, autophagy, mitochondrial function, and antioxidant capacity were measured. Results: The results showed that rutin could not reverse HZn-induced body weight decline but improved relative organ indexes in liver and kidney. It alleviated HZn-induced cell damage and enhanced antioxidant capacity in jejunum and serum through Nrf2 activation, without inhibiting HZn-induced zinc elevation. Conclusions: Rutin, especially at 600 mg\/kg, can partially restore hepatic function and organ index and mitigate HZn-induced hepatic and jejunal injuries.<\/jats:p>","DOI":"10.3390\/nu17091495","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T05:17:12Z","timestamp":1745903832000},"page":"1495","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Rutin Ameliorates Nanoparticle Zinc Oxide-Induced Toxicity in Mice by Potentiating Antioxidant Defense Mechanisms"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9635-5144","authenticated-orcid":false,"given":"Xiaofang","family":"He","sequence":"first","affiliation":[{"name":"College of Animal Science and Food Engineering, Jinling Institute of Science and Technology, Nanjing 211169, China"},{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longfei","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4562-4998","authenticated-orcid":false,"given":"Binbin","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minhang","family":"Tu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gentan","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"ref_1","first-page":"219","article-title":"Review on zinc oxide nanoparticles: Antibacterial activity and toxicity mechanism","volume":"7","author":"Sirelkhatim","year":"2015","journal-title":"Nanomicro Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.aninu.2016.06.003","article-title":"Nano zinc, an alternative to conventional zinc as animal feed supplement: A review","volume":"2","author":"Swain","year":"2016","journal-title":"Anim. 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